Peony seed meal polypeptide with antioxidant activity as well as preparation method and application thereof

A technology of peony seed meal and oxidation activity, which is applied in the field of peony seed meal polypeptide and its preparation, can solve the problems of unfavorable industrial production practical application, organic solvent pollution, etc., and achieve the effect of mild treatment conditions, low molecular weight and uniform molecular weight

Active Publication Date: 2018-11-16
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology provides an improved way for making edible oils that are resisting oxidative damage caused during storage or use. It involves creation of a special type called peanut oil seeds (Pseudomonas spp.) through genetic engineering techniques. These PSEs have lower levels of certain compounds than regular fishmeals but they still contain some important nutrients like carotenoids from plants such as tomatoes. They also include other substances found naturally within them - including flavonoids and tannins-that help protect against harmful reactions when eaten raw without being consumed beforehand. Overall these improvements make it easier to store and transport cookery ingredients while maintain their quality over time.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to improve the quality of vegetable materials without compromising their original properties during processing. Specifically, there is concern about developing biodegraded compounds with improved bioactivity while reducing harmful side effects caused by oxidative stressors like redoxygen defiuxion systems. Previous methods were either too complex or did not provide sufficient protection against these damaging agents.

Method used

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  • Peony seed meal polypeptide with antioxidant activity as well as preparation method and application thereof
  • Peony seed meal polypeptide with antioxidant activity as well as preparation method and application thereof
  • Peony seed meal polypeptide with antioxidant activity as well as preparation method and application thereof

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preparation example Construction

[0043] As introduced in the background technology, there are certain deficiencies in the peony seed meal polypeptide product and its preparation method in the prior art. In order to solve the above technical problems, the present invention proposes a preparation of peony seed meal polypeptide with high antioxidant activity method, the method includes the following steps:

[0044] (1) Using deoiled peony seed meal as raw material, adopting the method of acid precipitation and alkali dissolution to extract the protein of peony seed meal;

[0045] (2) According to the material-liquid ratio (0.5-3) g: (100-200) mL, mix the peony seed meal protein with water, and adjust the pH of the solution to 7-8;

[0046] (3) Add neutral protease to the solution described in step (2), and mix well, control the reaction temperature to 50°C-70°C, and continuously add 1mol / L NaOH solution during the reaction process, and enzymatically hydrolyze for 2-4h After that, inactivate the enzyme;

[0047] ...

Embodiment 1

[0076] Example 1 A preparation method of peony seed meal polypeptide with antioxidant activity

[0077] The present invention provides such figure 1 A method for preparing a peony seed meal polypeptide with antioxidant activity includes the following steps:

[0078] 1) Adjust the pH value of the deoiled peony seed meal with a solid-liquid ratio of 1:10 to 8, centrifuge to take the supernatant, then adjust the pH value of the supernatant to 3.6, after standing for a period of time, centrifuge to take the precipitate, and then add water to reconstitute The pH value of the solution is adjusted to 7, and then freeze-dried to obtain the peony seed meal protein.

[0079] 2) Mix peony seed meal protein material with distilled water according to the ratio of material to liquid: 0.5g:100mL, adjust the pH value of the solution to 7 with 1mol / L NaOH or HCl, and then place it in a constant temperature water bath.

[0080] 3) Add neutral protease to the solution described in step 2), the...

Embodiment 2

[0087] A preparation method of peony seed meal polypeptide with antioxidant activity, comprising the following steps:

[0088] 1) Adjust the pH value of the deoiled peony seed meal with a solid-liquid ratio of 1:10 to 8, centrifuge to take the supernatant, then adjust the pH value of the supernatant to 3.6, after standing for a period of time, centrifuge to take the precipitate, and then add water to reconstitute The pH value of the solution is adjusted to 7, and then freeze-dried to obtain the peony seed meal protein.

[0089] 2) Mix peony seed meal protein material with distilled water according to the ratio of material to liquid: 1g:120mL, adjust the pH value of the solution to 7.5 with 1mol / L NaOH or HCl, and then place it in a constant temperature water bath.

[0090] 3) Add neutral protease to the solution described in step 2), the amount of enzyme added is 0.1g (activity 0.8AU / g), and fully mixed, put into a water bath for reaction, the temperature is 55 ° C, and the re...

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Abstract

The invention relates to a peony seed meal polypeptide with antioxidant activity as well as a preparation method and application thereof. The method comprises the following steps that neutral proteaseand alkaline protease are subjected to synergistic multi-step enzymolysis, after enzymolysis is conducted for 48 h, centrifugal filtration is conducted, supernate is taken, then the supernate, namelyenzymolysis liquid, is subjected to an antioxidant test, and the enzymolysis liquid is subjected to freeze drying to obtain the peony seed meal polypeptide with the antioxidant activity. The method is simple to operate, treatment conditions are mild, after synergistic multi-step enzymolysis of the neutral protease and the alkaline protease, the purity of a peony seed polypeptide product is high,the molecular weight is even, through TricineSDSPAGE electrophoresis detection, the molecular weight of the peony seed polypeptide product is 5.8-6.2 KDa, and the purity is 75% or above; the antioxidant activity of the peony seed meal polypeptide in the molecular weight range is very outstanding, especially, the capability of the polypeptide of clearing ABTS<+> free radicals is very significant, and when the concentration of the polypeptide is 1 mg/mL, the clearance rate is up to 82%.

Description

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Claims

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Application Information

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Owner QILU UNIV OF TECH
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