Application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food
An anti-aging and anti-oxidation technology, applied in the preparation of food or health food, in the field of angelica peptides, can solve the problems of lack of antioxidant and anti-aging, less angelica polypeptides, etc., achieve significant antioxidant activity, reduce lipofuscin level, reducing the effect of deposits
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Embodiment 1
[0042] The angelica polypeptide provided in this example is prepared by the following method: after crushing the rhizome of Angelica sinensis, add Tris-HCl buffer solution according to the ratio of solid to liquid 1:5 (g / mL), buffer with pH 7.4, 20mmol / L Tris-HCl Extract protein from the solution, stir at room temperature for 12 hours, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clarified extract; add ammonium sulfate solid to the above extract to a saturation of 80%, mix well, and let stand overnight at room temperature to precipitate Centrifuge to collect the precipitate, use 3.5kD dialysis bag for dialysis to remove salt ions and small molecules, and the dialysis retentate is vacuum freeze-dried; the precipitate is dissolved in water according to the solid-liquid ratio of 1:20 (g / mL) and then add trypsin and Papain, inactivate the enzyme activity after each reaction for 4 hours at 45°C, pH 8.0 (trypsin enzymatic hydrolysis, the sam...
Embodiment 2
[0045] The angelica polypeptide provided in this example is prepared by the following method: after crushing the rhizome of Angelica sinensis, add Tris-HCl buffer solution according to the ratio of solid to liquid at 1:50 (g / mL), buffer with Tris-HCl with pH 8.0, 10mmol / L Extract the protein from the solution, stir at room temperature for 8 hours, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clarified extract; add ammonium sulfate solids to the above extract to a saturation of 50%, mix well, and let stand overnight at room temperature to precipitate ; centrifuge to collect the precipitate, use 5.0kD dialysis bag dialysis to remove salt ions and small molecules, and the dialysis retentate is vacuum freeze-dried; after the precipitate is dissolved in water according to the solid-liquid ratio of 1:20 (g / mL), add trypsin and Papaya enzyme was reacted at 37°C, pH 8.0 and 80°C, pH 6.0 respectively for 2 hours, then inactivated, and freeze-dr...
Embodiment 3
[0048] The angelica polypeptide provided in this example is prepared by the following method: after crushing the rhizome of Angelica sinensis, add Tris-HCl buffer solution according to the ratio of solid to liquid at 1:10 (g / mL), buffer with pH 9.0, 1mmol / L Tris-HCl Extract protein from the solution, stir at room temperature for 24 hours, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clarified extract; add ammonium sulfate solids to the above extract to a saturation of 90%, mix well, and set aside overnight at room temperature for precipitation Centrifuge to collect the precipitate, use 2.5kD dialysis bag for dialysis to remove salt ions and small molecules, and the dialysis retentate is vacuum freeze-dried; the precipitate is dissolved in water according to the solid-liquid ratio of 1:20 (g / mL) and then add trypsin and Papaya enzyme was reacted at 50° C., pH 8.0 and 70° C., pH 6.0 respectively for 8 hours, then inactivated, and freeze-...
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