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Application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food

An anti-aging and anti-oxidation technology, applied in the preparation of food or health food, in the field of angelica peptides, can solve the problems of lack of antioxidant and anti-aging, less angelica polypeptides, etc., achieve significant antioxidant activity, reduce lipofuscin level, reducing the effect of deposits

Inactive Publication Date: 2015-04-22
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is still little research and development on the activity and function of angelica polypeptide, and there is a lack of related research on its anti-oxidation and anti-aging aspects

Method used

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  • Application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food
  • Application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food
  • Application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The angelica polypeptide provided in this example is prepared by the following method: after crushing the rhizome of Angelica sinensis, add Tris-HCl buffer solution according to the ratio of solid to liquid 1:5 (g / mL), buffer with pH 7.4, 20mmol / L Tris-HCl Extract protein from the solution, stir at room temperature for 12 hours, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clarified extract; add ammonium sulfate solid to the above extract to a saturation of 80%, mix well, and let stand overnight at room temperature to precipitate Centrifuge to collect the precipitate, use 3.5kD dialysis bag for dialysis to remove salt ions and small molecules, and the dialysis retentate is vacuum freeze-dried; the precipitate is dissolved in water according to the solid-liquid ratio of 1:20 (g / mL) and then add trypsin and Papain, inactivate the enzyme activity after each reaction for 4 hours at 45°C, pH 8.0 (trypsin enzymatic hydrolysis, the sam...

Embodiment 2

[0045] The angelica polypeptide provided in this example is prepared by the following method: after crushing the rhizome of Angelica sinensis, add Tris-HCl buffer solution according to the ratio of solid to liquid at 1:50 (g / mL), buffer with Tris-HCl with pH 8.0, 10mmol / L Extract the protein from the solution, stir at room temperature for 8 hours, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clarified extract; add ammonium sulfate solids to the above extract to a saturation of 50%, mix well, and let stand overnight at room temperature to precipitate ; centrifuge to collect the precipitate, use 5.0kD dialysis bag dialysis to remove salt ions and small molecules, and the dialysis retentate is vacuum freeze-dried; after the precipitate is dissolved in water according to the solid-liquid ratio of 1:20 (g / mL), add trypsin and Papaya enzyme was reacted at 37°C, pH 8.0 and 80°C, pH 6.0 respectively for 2 hours, then inactivated, and freeze-dr...

Embodiment 3

[0048] The angelica polypeptide provided in this example is prepared by the following method: after crushing the rhizome of Angelica sinensis, add Tris-HCl buffer solution according to the ratio of solid to liquid at 1:10 (g / mL), buffer with pH 9.0, 1mmol / L Tris-HCl Extract protein from the solution, stir at room temperature for 24 hours, centrifuge to remove residual medicinal residues and insoluble impurities, and obtain a clarified extract; add ammonium sulfate solids to the above extract to a saturation of 90%, mix well, and set aside overnight at room temperature for precipitation Centrifuge to collect the precipitate, use 2.5kD dialysis bag for dialysis to remove salt ions and small molecules, and the dialysis retentate is vacuum freeze-dried; the precipitate is dissolved in water according to the solid-liquid ratio of 1:20 (g / mL) and then add trypsin and Papaya enzyme was reacted at 50° C., pH 8.0 and 70° C., pH 6.0 respectively for 8 hours, then inactivated, and freeze-...

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Abstract

The invention discloses an application of Chinese angelica polypeptide with effects of resisting oxidization and delaying ageing in preparation of food, particularly of health food, and further discloses a preparation method of the Chinese angelica polypeptide. The preparation method is simple and easy to implement, safe and non-toxic, stable and efficient, and is suitable for industrial production. In vitro, the Chinese angelica polypeptide has a characteristic of a certain hydroxyl radical clearance rate, which means that the Chinese angelica polypeptide has capacity of clearing active oxygen; at an integral animal level, an oxidative stress model and an ageing-associated model are used to perform a test, the test result discloses that the survival time of caenorhabditis elegans under an oxidative stress condition can be remarkably prolonged, the lipofuscin level in the caenorhabditis elegans is reduced, average lifetime of the caenorhabditis elegans is prolonged; under the oxidative stress condition and the ageing condition, the Chinese angelica polypeptide can be used for realizing remarkable anti-oxidization and ageing delaying effects by lowering the reactive oxide species (ROS) in vivo and lowering the content of lipid peroxide.

Description

technical field [0001] The invention belongs to the technical field of food, and further belongs to the technical field of health food (also referred to as health food or functional food), and specifically relates to the application of an angelica polypeptide with anti-oxidation and anti-aging effects in the preparation of food or health food. technical background [0002] With the aging trend of the global population increasing year by year, the development of aging-related diseases is becoming increasingly serious, becoming a major disease that seriously endangers human life, health and quality of life, and has attracted extensive and in-depth attention in the international biomedical field. Although the mechanism of occurrence and development of aging and related diseases is complex, a large number of studies have shown that oxidative stress damage is one of its important characteristics. According to the free radical theory of aging, when the organism is in an oxidative ...

Claims

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Application Information

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IPC IPC(8): A23L1/305A23J3/34A23J3/14A23L33/18
CPCA23J3/346A23J3/14A23V2002/00A23V2200/30
Inventor 马忠华梁明郭晓蕾毛新亮马方励
Owner INFINITUS (CHINA) CO LTD
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