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Preparation method of natural antioxidant tricholoma lobayense heim polysaccharide

A technology of natural antioxidant, jinfuji polysaccharide, which is applied in the field of food processing and achieves the effect of broad application prospects

Inactive Publication Date: 2012-07-25
ANHUI CHINATURE BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

F.Liu (Gen.Pharmac.1996) showed that Flammulina glycoprotein has immune regulation and anti-tumor activity through in vivo and in vitro tumor inhibition experiments; H.X.Wang (Comp.Biochem.Physiol.Part B.2000) described the The 30kDa protein has the activity of inhibiting in vitro translation; however, there is no literature report on the research on the antioxidant activity of jinfu mushroom polysaccharide.

Method used

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  • Preparation method of natural antioxidant tricholoma lobayense heim polysaccharide
  • Preparation method of natural antioxidant tricholoma lobayense heim polysaccharide
  • Preparation method of natural antioxidant tricholoma lobayense heim polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Determination of the crude polysaccharide extraction process with extraction rate as reference

[0027] According to the results of the preliminary test, three factors that have a significant impact on the extraction rate of polysaccharides are selected: solid-liquid ratio, extraction time, and extraction temperature, and on the basis of single-factor experiments, the best response is analyzed by three-factor three-level response surface analysis method Value, experimental design, analysis scheme and yield results are shown in Table 1 and Table 2.

[0028] Table 1 Response surface analysis factors and levels table

[0029]

[0030]

[0031] Table 2 Response surface analysis scheme and yield results

[0032]

[0033] *: (mass of dry polysaccharide / mass of raw material)×100%.

[0034] After regression fitting of each factor, the regression equation is solved as follows:

[0035] Y=12.92+0.2625A+0.3625B+0.625+0.075AB+0.55AC-0.05BC-1.2975A 2 -1.34...

Embodiment 2

[0037] Example 2: Preparation of Jinfu mushroom polysaccharide

[0038] Bake 500g of the fruiting body of Jinfu mushroom at 46°C for 24 hours, then at 52°C for 24 hours, the weight is 75g, continue to bake at 37°C until the constant weight is 63g, crush it, and pass through a 0.55mm sieve.

[0039]Weigh 20g of Jinfu mushroom powder, extract in hot water (temperature 83°C, material-to-liquid ratio 1:35, time 2.5h), concentrate the extract with a rotary evaporator, centrifuge at 3500r / min for 20min, take the supernatant, and concentrate to After 1 / 10 of the original volume, add 4 times the volume of absolute ethanol to precipitate, leave it overnight, centrifuge at 3500r / min for 20min, collect the precipitate, freeze-dry, and obtain 2.451g of light brown golden blessing mushroom crude polysaccharide, the extraction rate is 12.25% .

[0040] Weigh 0.5 g of crude polysaccharide, dissolve it in 10 mL of distilled water, add Sevag reagent (chloroform: n-butanol = 4: 1, v / v) at a vo...

Embodiment 3

[0043] Embodiment 3: Determination of antioxidant activity in vitro:

[0044] 1. Determination of inhibition of pyrogallol autoxidation

[0045] Take 4mL of 0.05mol / LTris-HCl buffer solution (pH8.2), place it in a water bath at 25°C to preheat for 20min, and add different concentrations (50, 100, 200, 400, 500μg / mL) of the polysaccharide fraction 1mL, preheat the above mixed solution to 25°C, add 0.3ml of 25°C preheated 45mmol / L pyrogallol solution before measurement, mix quickly, and set the absorbance at 420nm. For treatment, replace the sample with the same volume of distilled water. Positive control is the same concentration of Vc aqueous solution. The default is the absorbance value of the first minute, and the absorbance value is read every 1 minute within 4 minutes, and the absorbance values ​​of samples with different concentrations at different times are recorded. The inhibition rate formula of Jinfu mushroom polysaccharide: Y=(Fo-Fx) / Fo. Where Y is the clearance ...

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Abstract

The invention discloses a preparation method of a natural antioxidant tricholoma lobayense heim polysaccharide, comprising the following steps of: drying tricholoma lobayense heim until the tricholoma lobayense heim has a constant weight and then smashing and sieving; then digesting obtained powder with hot water, rotatably evaporating and concentrating an extracting solution, and taking liquid supernatant after centrifuging; and finally, concentrating the liquid supernatant, taking sediments after ethanol precipitation and centrifuging, and freezing and drying, so as to obtain the tricholoma lobayense heim polysaccharide. The tricholoma lobayense heim polysaccharide is obtained by extracting is a natural product with high antioxidant activity and can be used for obviously cleaning.O2<-> generated by a pyrogallic acid self-oxidation system, particularly a component of TL-3. When the concentration is 500 microgram / mL, the clearance rate can reach 75.6%, which is only 15.4% lower than Vc. Therefore, the tricholoma lobayense heim polysaccharide can be used as a natural non-toxic antioxidant in fields such as foods, medicines, cosmetics and the like.

Description

technical field [0001] The invention relates to a preparation method of natural antioxidant jinfu mushroom polysaccharide, which belongs to the field of food processing. Background technique [0002] Fungi have been receiving much attention because of their nutritional and medicinal value, and a variety of compounds with pharmacological effects have been isolated from fungi (such as lentinan). Polysaccharides have important biological activities in terms of immune regulation, enhancing cell anti-tumor, and antioxidant activity. A large number of studies have proved that most fungal polysaccharides have antioxidant activity, such as: polysaccharides from fungus (Mau, 2001), polysaccharides from Cordyceps sinensis (S.P.Li, 2006), polysaccharides from Ganoderma lucidum (Jing Xu, 2009), etc. The research and application of fungal polysaccharides have penetrated into many fields such as medicine, food, cosmetics and feed, and polysaccharide research has become one of the most ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00G01N30/02A23L3/3562
Inventor 陈彦王翠王瑞君胡美丽段培鲁翟天龙
Owner ANHUI CHINATURE BIOLOGICAL CO LTD
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