The invention relates to a preparation method of health-care yoghourt rich in vitamin C, and belongs to the technical field of application of vitamin C. The preparation method comprises the following steps of (1) mixing milk powder with water so as to obtain milk liquid; (2) adding cane sugar and vitamin C composite emulsion to the milk liquid, performing dissolving, and performing homogenizing; (3) sterilizing the solution obtained in the step (2), and performing cooling; and (4) adding zymocyte, performing canning, performing sealing, performing constant-temperature fermentation, performing refrigeration, and performing after-ripening so as to obtain the health-care yoghourt rich in vitamin C, wherein the vitamin C composite emulsion is WOW type emulsion prepared by embedding vitamin C through a two-step emulsification method. The preparation method disclosed by the invention is simple and convenient to operate and high in preparation efficiency, the action time of active substances is prolonged, the storage stability and the using stability of the vitamin C are improved, the bioavailability of the vitamin C is increased, and the prepared yoghourt is good in mouth feel, and has the efficacy of enhancing the immunity of organisms and beautifying faces.