56results about How to "Improve encapsulation efficiency" patented technology

The present invention provides novel calcium phosphate nanoparticles suitable for efficient encapsulation of biologically active molecules. The invention further provides pharmaceutical compositions comprising these nanoparticles, as well as methods of making such nanoparticles and using them as carriers for therapeutic delivery of biologically active macromolecules.

The invention discloses a polypeptide or protein nanoparticle based on hydrogen-bonded complexation and a preparation method and application thereof. The nanoparticles comprise a polyphenol compound carrier such as tannic acid as a hydrogen bond donor, a polypeptide or a protein drug such as a glucagon-like peptide as a hydrogen bond acceptor 1 analog (exenatide), salmon calcitonin or insulin, etc., and chitosan, polyvinylpyrrolidone or hyaluronic acid, etc. coated on that surface of the particle as stabilizer and / or functional molecule. The nanoparticles of the invention have extremely high encapsulation efficiency and drug loading capacity, and the prepared therapeutic polypeptide or protein drug nanoparticles are suitable for oral delivery or subcutaneous injection application, and havegreat application prospect.

The invention provides a method for preparing hydrophobic vitamin microcapsules, and relates to vitamin microcapsules. The method comprises the following steps: (1) adding an oil-phase core material in an agitating pan, and melting the oil-phase core material to prepare vitamin oil containing antioxidant, wherein the oil-phase core material comprises lipid-soluble vitamin, an antioxidant and other greases; (2) dissolving a wall material in water to prepare a water solution with solid content of 20-60wt% to obtain a water phase; (3) adding the vitamin oil containing antioxidant prepared in the step (1) into the water phase obtained in the step (2), and emulsifying and homogenizing to form uniform emulsion; (4) adding protein active enzyme into the emulsion prepared in the step (3), granulating, performing a crosslinking reaction, and drying to obtain the hydrophobic vitamin microcapsules. According to the method, other toxic or strong oxidizing chemical substances are not added, the prepared microcapsules are safer and more environment-friendly, has longer shelf life, and is high in embedding rate and low in surface oil, so that the microencapsulation efficiency can be further improved.

A submicron emulsion of paclitaxel, the preparation method and the use thereof are disclosed. Said paclitaxel submicron emulsion comprises paclitaxel / steroid complex, oil for injection, water for injection, emulsifier, assistant emulsifier and isotonic agent, wherein the mole ratio of paclitaxel to steroid in the complex is 1:0.2˜4; preferably 1:0.25˜2. Said submicron emulsion is useful for the treatment for malignant tumor. The average particle diameter of the submicron emulsion is less than 400 nm and the pH Value is 3.5-6.

The invention discloses a preparation method of a liposome medicine with the outer layer modified by a hydrophilic polymer. The method comprises the following steps of: preparing a liposome, and then inserting a lipid substance modified by a hydrophilic polymer outside two layers of the phospholipid. The method not only can achieve high encapsulation rate, but also can realize the slow release and target action of a long circulating liposome.

The invention discloses photosensitizer targeting nanoparticles for optical therapy as well as a preparation method and applications of the photosensitizer targeting nanoparticles. The general formula of the targeting nanoparticles is HA-PAMAM-COOH-ICG, wherein HA is hyaluronic acid, PAMAM is a dendritic polymer, COOH is carboxyl, and ICG is indocyanine green. The nanoparticles provided by the invention are good in stability, realize the advantage that the local concentration of the medicine is high through the targeting effect after being administrated, are basically free of toxic and side effects, can be used for carrying out fluorescence imaging research on the accumulation condition of the medicine in a tumor, and also can be used as a photosensitizer for exerting the curative effects of PTT / PDT.

The invention discloses curcumin nano-particles and a preparation method thereof. The curcumin nano-particles compounded by BSG protein extract and lecithin are prepared with a base and / or alcohol extracted BSG protein extract as carrier of curcumin. The prepared curcumin nano-particles are spherical and uniform, and have good dispersibility, narrow PDI value and high encapsulating efficiency. Thecurcumin is wrapped in a hydraophobic nuclear of the BSG protein extract, and solubility, heat stability and ultraviolet radiation stability of the wrapped curcumin are remarkably improved; and besides, the wrapped curcumin has higher DPPH free radical scavenging activity than free curcumin.

The invention relates to the field of medicinal preparations, in particular to a target and fluorescence dual-functional slightly-soluble antitumor medicament nano structural lipid carrier. In the slightly-soluble antitumor medicament nano structural lipid carrier, a lipid carrier of which the inside carries medicaments is formed by solid lipid, liquid lipid, surfactants and slightly-soluble antitumor medicaments; and the surface of the lipid carrier is chemically modified and is in covalent connection with a ligand with a tumor target. The slightly-soluble antitumor medicament nano structural lipid carrier can prolong the in-vivo circulation time of the slightly-soluble antitumor medicaments and improve the bioavailability of the slightly-soluble antitumor medicaments and can be used fortopical diagnosis of tumors by fluorescence imaging.

The invention provides a colon-targeted gel microsphere with a controllable core-shell ratio, and preparation and application of the colon-targeted gel microsphere. The gel microsphere comprises a core layer, an inner shell layer and an outer shell layer from inside to outside in sequence, wherein the core layer comprises cereal prolamin and an active matter; the inner shell layer comprises gel polysaccharide; the outer shell layer comprises chitosan, a cross-linking agent and a suspending aid. The inner shell layer and the core layer form core-shell structure fog drops through a coaxial electrostatic spraying technology, in an outer shell layer solution, the inner shell layer gel polysaccharide of the core-shell structure fog drops is cross-linked with the cross-linking agent and is subjected to electrostatic layer-by-layer adsorption with chitosan, and therefore, the colon-targeted gel microsphere is prepared. The particle size of the gel microsphere is 100-600 [mu] m, the core-shell ratio is 0.5-0.9, the encapsulation efficiency of a water-soluble active matter is as high as 60%, the encapsulation efficiency of an alcohol-soluble active matter is as high as 90%, the release amounts in a stomach simulation liquid and a small intestine simulation liquid within 1 h and 3 h can be as low as 10% and 25%, and the gel microsphere can be efficiently delivered and targeted to the colon.

The invention discloses a preparation method and application of a reduction-sensitive drug delivery system with a high drug loading rate, belonging to the technical field of biomedicine and nanomedicine. According to a technical scheme in the invention, 2,2-dihydroxyethyl disulfide is bonded with camptothecin via a carbonate bond to construct a camptothecin dimer with reduction sensitivity; and then the camptothecin dimer is encapsulated into an amphiphilic block copolymer polyethylene glycol-polycaprolactone by using a dialysis method so as to prepare the reduction-sensitive drug delivery system with a high drug loading rate, wherein the camptothecin dimer has a structure formula as described in the specification. The invention also specifically discloses application of the reduction-sensitive drug delivery system with a high drug loading rate to preparation of sustained-release and controlled-release drugs with targeted anti-cancer effect. Compared with a camptothecin delivery system, the drug loading rate of the drug delivery system provided by the invention is increased from 1% to 12.6%, and the drug delivery system has reduction sensitivity, good biocompatibility and capacityof inhibiting proliferation of tumor cells.

The invention discloses a traditional Chinese medicine composition with bacteriostatic, anti-inflammatory, hemostatic and analgesic effects and a preparation method thereof. According to the method, apithecellobium clypearia extract and a lamiophlomis rotata extract are used as raw materials; poly(lactic-co-glycolic acid) PLGA, vitamin E polyethylene glycol succinate TPGS and poloxamer are used as a drug-loaded matrix. The preparation method comprises the following steps: mixing raw medicinal materials with the drug-loaded matrix to form an oil phase and an oil-in-water phase, performing probe ultrasonic emulsification to obtain a nano-suspension, and adding carbomer, collagen tripeptide, oat beta-glucan and propylene glycol as a gel matrix to obtain traditional Chinese medicine composition nanogel. The prepared traditional Chinese medicine composition nanogel has the particle size of 120 nm-260 nm, has good drug loading capacity, obviously improves the solubility and bioavailabilityof hydrophobic drugs, inhibits bacteria, diminishes inflammation, stops bleeding and relieves pain, and is definite in curative effect.

The invention discloses a preparation method of an expanded vermiculite paraffin PAM shape-stabilized phase change energy storage material. The preparation method comprises the following steps: preparing paraffin / PAM emulsion; and adding the expanded vermiculite into the paraffin / PAM emulsion to obtain the expanded vermiculite paraffin PAM shape-stabilized phase change energy storage material. Theexpanded vermiculite has strong hydrophilicity and low density, can be used as an additive to be added into the body of the paraffin / PAM shape-stabilized phase change material, reduces the flow of paraffin in the material, reduces the leakage amount of paraffin, and improves the utilization efficiency of paraffin. By adding the expanded vermiculite, the leakage of system paraffin is effectively reduced, and the paraffin encapsulation efficiency of the paraffin / PAM shape-stabilized phase change material is improved; in addition, the leakage of the paraffin is effectively reduced, and the encapsulation efficiency of the paraffin and the cycling stability of the material are improved.

The invention discloses a preparation method of a hydrated inorganic salt expanded vermiculite paraffin PAM composite phase change energy storage material. The preparation method comprises the following steps: (1) preparing hydrated inorganic salt / expanded vermiculite by a physical impregnation method; (2) preparing a paraffin / PAM emulsion; (3) adding the hydrated inorganic salt / expanded vermiculite into the paraffin / PAM emulsion to prepare the hydrated inorganic salt / expanded vermiculite with addition of the hydrated inorganic salt / expanded vermiculite paraffin / PAM shape-stabilized phase change energy storage material. The invention effectively reduces the leakage of paraffin as well, improves the encapsulation efficiency of paraffin, and further improves the phase change latent heat of the material due to the existence of the hydrated inorganic salt; more importantly, the hydrated inorganic salt is compounded into the body of the paraffin / PAM shape-stabilized phase change material, so that the supercooling degree of the hydrated inorganic salt is effectively reduced. Test results show that the composite phase change material has high latent heat of phase change, good thermal stability and cycling stability, and good application prospect.

The present invention relates to a hybrid-type multi-lamellar nanostructure of an epidermal growth factor and a liposome and a method for manufacturing same. The new kind of hybrid-type multi-lamellar nanostructure not only has a high epidermal growth factor sealing efficiency, but also can be manufactured through a simple process, such that the same can be easily delivered into a living body or a cell while maintaining a high physiological activity of the epidermal growth factor.

The invention discloses a preparation method of a nano-particle rapamycin-loaded drug coating balloon, and belongs to the technical field of drug manufacturing. The surface of the balloon is coated with hollow mesoporous silica nano-particles (MSNs) loaded with Rapamycin (RPM) through polylactic acid-polyglycolic acid (PLGA). The polylactic acid-polyglycolic acid mixes the mesoporous silica nano-particles loaded with rapamycin into the surface of the balloon, the surface of the balloon is coated with the polylactic acid-polyglycolic acid, nano-particles are bridged to prepare the nano-particle rapamycin-loaded drug coating balloon which is excellent in histocompatibility, biodegradable, high in encapsulation efficiency, stable in drug loading capacity control, small in particle size and constant in-vitro drug release, the effective action concentration of the drug can be reached and maintained in local tissue under the condition that blood flow is affected as little as possible, the negative influence of a drug loading matrix on vascular repair can be overcome, and the anti-endometrial hyperplasia and anti-restenosis effects of rapamycin are improved. The inorganic nano-particles can be compounded with an up-conversion rare earth luminescent fluorescent agent, 4f electrons of a rare earth inner shell layer can emit light under laser irradiation, and therefore the nano-particle rapamycin-loaded drug coating balloon is used for simulating in-vitro conveying and expansion of the balloon in plasma and monitoring the concentration of the nano-particles left in the plasma and used for detecting the drug loading stability of the balloon in the early stage.

A composition of goserelin sustained release microspheres is provided. The microspheres comprise goserelin, at least one poly(lactide-co-glycolide) and poloxamer or PEG. The sustained release microspheres have comparatively high bioavailability, which promotes the drug taking its full effect and have entrapment efficiency over 90%.

The invention relates to the field of pharmacy, and discloses a liposome composition capable of adjusting drug release and a preparation method thereof. The liposome composition includes liposome, media including a water phase encapsulated in the liposome and a compound; the media include at least one polyamino acid or salt thereof, and at least one therapeutic agent; and the compound can generateion gradient inside and outside of the liposome and make the therapeutic agent encapsulate the water phase in the liposome. Through the introduction of the polyamino acids or salts thereof into the water phase in the liposome, the release situations of drugs in the liposome can be adjusted, and the adjustment effect has a certain dose dependent. The preparation method mainly relates to an activedrug loading method which can form the ion gradient inside and outside of the liposome; and the polyamino acids or salts thereof can be directly taken as an internal water phase to introduce. The preparation method is simple in technology, convenient in operation and less in control parameter, so that the reduction of production costs can be realized, and the release of the drugs in the liposome can be effectively adjusted.

The invention relates to the technical field of traditional Chinese medicines, in particular to a medicine composition, and a preparation method and application thereof. The medicine composition comprises the following ingredients in parts by weight: 80-90 parts of fructus forsythia, 60-70 parts of fermented soya beans, 40-50 parts of herba menthae, 40-50 parts of herba schizonepetae, 30-40 partsof stir-fried fructus gardenia, 30-40 parts of radix rhei, 60-70 parts of herba artemisiae annuae, 40-50 parts of radix paeoniae rubra, 30-40 parts of semen arecae, 60-70 parts of cortex magnoliae officinalis, 60-70 parts of scutellariae radix, 60-70 parts of rhizoma pinelliae, 40-50 parts of radix bupleuri, 30-40 parts of glycyrrhizae radix, 1-5 parts of preservatives, 1-5 parts of sweetening agents and 1-5 parts of essence. The medicine composition has the advantages of a high effective ingredient content, high stability and good child patient eating compliance.

The invention discloses an siRNA delivery carrier and a preparation method and application thereof. The siRNA delivery carrier comprises a nano vesicle and siRNA loaded in the nano vesicle, and the nano vesicle is prepared from an erythrocyte membrane. The invention further discloses an siRNA delivery carrier based on nucleic acid aptamer targeting, which comprises a nano vesicle, siRNA loaded in the nano vesicle and the cholesterol modified nucleic acid aptamer loaded on the surface of the nano vesicle. The nano vesicle is prepared from an erythrocyte membrane. In the siRNA delivery carrier based on nucleic acid aptamer targeting, which is prepared by the invention, the siRNA is loaded in the nano vesicle, the encapsulation efficiency is higher, and the siRNA delivery carrier is difficult to degrade.

The invention relates to a preparation method of health-care yoghourt rich in vitamin C, and belongs to the technical field of application of vitamin C. The preparation method comprises the following steps of (1) mixing milk powder with water so as to obtain milk liquid; (2) adding cane sugar and vitamin C composite emulsion to the milk liquid, performing dissolving, and performing homogenizing; (3) sterilizing the solution obtained in the step (2), and performing cooling; and (4) adding zymocyte, performing canning, performing sealing, performing constant-temperature fermentation, performing refrigeration, and performing after-ripening so as to obtain the health-care yoghourt rich in vitamin C, wherein the vitamin C composite emulsion is WOW type emulsion prepared by embedding vitamin C through a two-step emulsification method. The preparation method disclosed by the invention is simple and convenient to operate and high in preparation efficiency, the action time of active substances is prolonged, the storage stability and the using stability of the vitamin C are improved, the bioavailability of the vitamin C is increased, and the prepared yoghourt is good in mouth feel, and has the efficacy of enhancing the immunity of organisms and beautifying faces.

The invention discloses a preparation method and application of nanogel with a hunger combined gas therapy anti-tumor function. The nanogel is prepared by the following steps: firstly, performing electrostatic interaction on polyethyleneimine azide grafted with L-arginine and glucose oxidase to form a nano-enzyme compound; and then covering the surface of the nano-enzyme compound with an alkynyl-containing polycarbonate block copolymer, and performing click chemical crosslinking on azide and alkynyl. Compared with the prior art, according to the method disclosed by the invention, specific enzymatic reaction is utilized to consume endogenous glucose and release nitric oxide gas, cancer cells are killed through hunger and gas therapy, besides, the nitric oxide gas can redistribute oxygen in the cancer cells, hypoxia of tumors is relieved, and wide application prospects are realized in the aspects of resisting tumors and inhibiting tumor metastasis.

The invention discloses a thrombin-responsive reticular polymer and an application thereof in closed-loop thrombin inhibitor delivery, and belongs to the technical field of pharmaceutical preparations. The thrombin-responsive reticular polymer is obtained by a polymerization reaction of a polymer monomer, a thrombin degradable peptide and a thrombin targeting ligand. The polymer is used for entrapping a thrombin inhibitor, can adaptively and quickly release the loaded thrombin inhibitor under the action of high-concentration thrombin, can slowly release the thrombin inhibitor under low concentration, and is finally used for closed-loop thrombin inhibitor delivery, so that the antithrombotic activity of the thrombin inhibitor is improved, and adverse reactions are reduced.

The invention provides a rhein-loaded lactoferrin nanoparticle and a preparation method and application thereof, and belongs to the technical field of biomedical materials. Specifically, the inventionprovides a drug-loaded lactoferrin nanoparticle which is a nanoparticle with a three-layer structure, the three-layer structure comprises a pectin layer, a hyaluronic acid layer and a medicine-carrying lactoferrin layer from outside to inside. The medicine is preferably rhein. The drug-loaded dual-targeting drug delivery nanoparticle provided by the invention has good biocompatibility and good stability, can be orally taken, and has good targeting ability. The nanoparticle loaded with rheinic acid has good colon lesion inflammation and colon injury targeting ability. The nanoparticle maintains stability in stomach and small intestine during oral administration, slowly releases drugs, continuously releases drugs after targeting colon lesion sites, and has good treatment effect on colitis,especially ulcerative colitis. The nanoparticle is excellent in treatment effect and has a good application prospect.

The invention relates to a preparation method of epsilon-polylysine-Arabic gum nanoparticles, which comprises the following steps: (1) raw material preparation: taking epsilon-polylysine and Arabic gum powder, dissolving, filtering and diluting to obtain a diluent for later use; (2) ionic crosslinking: taking the diluent of epsilon-polylysine and Arabic gum in the step (1), and placing the diluent on a magnetic stirrer for mixing and stirring at room temperature; (3) thermal induction: taking the epsilon-polylysine-Arabic gum solution subjected to ionic crosslinking in the step (2), and heating in a water bath; (4) performing ultrasonic treatment: taking the epsilon-polylysine-Arabic gum solution subjected to thermal induction in the step (3), and performing ultrasonication to prepare a nanoparticle solution; and (5) drying for a finished product: taking the epsilon-polylysine-Arabic gum nanoparticle solution subjected to ultrasonic treatment in the step (4), and performing spray drying or freeze drying to obtain the finished product of epsilon-polylysine-Arabic gum nanoparticles. According to the invention, the encapsulation efficiency of the nanoparticles is improved, and the utilization rate of epsilon-polylysine is improved.

The invention discloses an acid-responsive crosslinked polymer prodrug and its preparation method and application. The acid-responsive crosslinked polymer prodrug is mainly composed of vinyl alkyl ether acrylate monomers and derivatives thereof The drug is linked with a hydroxyl-containing chemotherapeutic drug through an acid-responsive acetal bond to form a drug-small molecule complex, and then connected with a thiolated PEG through a Michael addition reaction to obtain the acid-responsive cross-linked polymer prodrug. Compared with the prior art, the present invention prepares acid-responsive cross-linked polymer prodrug micelles with high drug loading and high stability by modifying chemotherapeutic drugs, which has broad application prospects in antitumor.

The invention discloses a technological preparation method of anti-virus oral liquid. The technological preparation method comprises the following steps: pretreating Chinese herbal raw materials, performing distilling extraction to collect a volatile oil emulsion, concentrating the extracting solution, performing alcohol precipitation and concentration, and preparing the liquid, wherein before liquid is prepared, an ultrasonic-gel grinding method is adopted to include the volatile oil emulsion by using colloidal silicon dioxide under the assistance of water-soluble cyclodextrin. Compared withthe inclusion method using the cyclodextrin singly in the prior art, the technological preparation method provided by the invention has the advantages that the encapsulating efficiency is higher and the heating temperature in the inclusion process is relatively low; the volatile oil component is retained to the greatest extent; the equipment investment is small; the technological preparation method is suitable for industrial mass production in an enterprise. The prepared anti-virus oral liquid can obviously improve the stability of the volatile oil, so that the therapeutic effect of a productis ensured.

Provided are a nanoliposome-microbubble conjugate in which a drug for hair loss treatment such as finasteride, minoxidil, dutasteride, etc. is encapsulated in a nanoliposome and a composition for alleviating or treating hair loss containing the same. Since an orally administered agent such as finasteride, currently useful as a drug for hair loss treatment, causes side effects, drug delivery through scalp application is most desirable, but the drug is not delivered to hair follicle cells through scalp application alone. Since a drug for hair loss treatment useful as an external preparation for skin causes various side effects, the concentration thereof that is used needs to be further lowered. The above nanoliposome-microbubble conjugate is capable of increasing the delivery efficiency of a drug for hair loss treatment at a low concentration, and is thus very effective at treating androgenic alopecia.