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Affinity toxin targeting molecule of HPV16E7 affinity loaded granzyme B and application thereof

A HPV16E7, targeting molecule technology, applied in non-active ingredients medical preparations, medical preparations containing active ingredients, enzymes, etc., can solve the problem of immunogenicity limiting clinical application and so on

Pending Publication Date: 2020-03-06
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The toxins carried by antibodies mainly come from exogenous toxins such as bacteria and plants, such as diphtheria exotoxin and Pseudomonas aeruginosa toxin, etc., but the side effects caused by immunogenicity limit its clinical application

Method used

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  • Affinity toxin targeting molecule of HPV16E7 affinity loaded granzyme B and application thereof
  • Affinity toxin targeting molecule of HPV16E7 affinity loaded granzyme B and application thereof
  • Affinity toxin targeting molecule of HPV16E7 affinity loaded granzyme B and application thereof

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Embodiment Construction

[0040] The present invention discloses the affinity toxin targeting molecule loaded with granzyme B by HPV16E7 Affimer and its use, which is based on the specific Affibody with high affinity to HPV16E7 (namely: HPV16E7 Affibody) after affinity screening Based on this, by utilizing the dual advantages of the targeting affinity properties of the affimer and the cytotoxicity of granzyme GrB, an affinity toxin targeting HPV16E7 to exert cytotoxicity is designed and prepared. For the content of specific affimers with high affinity for HPV16E7, refer to Chinese patent ZL ZL201510028505.3, authorized announcement number: CN105859846B "Polypeptides with Binding Affinity for HPV16E7 and Their Uses".

[0041] The HPV16E7 affibody-loaded granzyme B affinity toxin targeting molecule of the present invention is an HPV16E7 affibody; it is connected with granzyme B obtained after the 201st amino acid is mutated and the basic amino acid is replaced by a non-polar amino acid at the same time; o...

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Abstract

The invention discloses an affinity toxin targeting molecule of HPV16E7 affinity body loaded granzyme B and an application thereof, the granzyme B loaded by the affinity toxin targeting molecule belongs to endogenous protein, the immunogenicity problem caused by heterologous protein is avoided, and the affinity toxin targeting molecule has a wider application prospect; in addition, the invention also provides the application of the affinity toxin targeting molecule as a drug and the like.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to an HPV16E7 affinity body-loaded granzyme B-loaded affinity toxin targeting molecule and its application. Background technique [0002] At present, infection of high-risk types such as human papilloma virus (HPV) 16 is closely related to the occurrence and development of cervical cancer, accounting for more than 65% of cervical cancer cases worldwide. Although the successful marketing of HPV vaccines has greatly reduced the incidence and mortality of cervical cancer in some developed countries, developing countries including China still have no effective measures against HPV infection and its resulting precancerous lesions and metastasis. Immunosuppression, immune non-response, immune immunity, etc. exist in the tumor microenvironment, and it is not easy for the body to rely on active immunity to eliminate diseased cells. Therefore, targeted intervention and control of cervi...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62C12N15/70A61K47/64A61K38/48A61P31/20A61P35/00A61P35/04G01N33/574G01N33/569
CPCC07K14/31C12N9/6467A61K47/64A61K38/482A61P31/20A61P35/00A61P35/04G01N33/57484G01N33/56983C12Y304/21079
Inventor 张丽芳李文姝汪文寰谢自新陈薪安朱珊丽蒋朋飞
Owner WENZHOU MEDICAL UNIV
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