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Method for preparing ephrin-B2 peripheral nerve injury repair promotion stent

A peripheral nerve injury, peripheral nerve technology, applied in the field of medical devices, can solve the problems of hard materials, difficult to prepare into a very standard shape, etc., to avoid immunogenicity, good nerve defect repair effect, and high difficulty.

Inactive Publication Date: 2011-05-25
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the material prepared by PLGA alone is too hard to be prepared into a very standardized shape, so PLGA is best mixed with other materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Take 10mm (width) × 50mm (length) of skin from the patient’s wound, remove the epidermis and subcutaneous tissue, and cut it into a film of 10mm (width) × 1mm (thickness) × 50mm (length) , after routine decellularization and freeze-drying, it was used as material I for later use;

[0028] 2) Take a 10 mm long nerve along the broken end from the damaged part of the peripheral nerve of the patient, and obtain Schwann cells by conventional in vitro culture;

[0029] 3) Material I was processed into a fiber shape of 0.5mm (width)×0.5mm (thickness)×50mm (length), soaked in ephrin-B2 protein aqueous solution with a concentration of 0.1μg / L for 10min, freeze-dried, and then Soak in the chloroform solution of PLGA with a concentration of 10g / L, drain the chloroform and use it as material II for later use;

[0030] 4) Mix material Ⅱ with Schwann cell suspension (cell concentration is 1×10 5 pc / ml) after 3 days of co-cultivation, it will be used as material III for later use...

Embodiment 2

[0034] 1) Take 10mm (width) × 60mm (length) of skin from the patient’s wound, remove the epidermis and subcutaneous tissue, and cut it into a film of 10mm (width) × 0.5mm (thickness) × 60mm (length) , after routine decellularization and freeze-drying, it was used as material I for later use;

[0035] 2) A 5 mm long nerve was taken from the damaged part of the peripheral nerve of the patient along the broken end, and Schwann cells were obtained by conventional in vitro culture;

[0036] 3) Process material Ⅰ into a fiber shape of 0.1mm (width)×0.1mm (thickness)×60mm (length), soak in ephrin-B2 protein aqueous solution with a concentration of 0.01μg / L for 10min, freeze-dry, and then Soak in the chloroform solution of PLGA with a concentration of 50g / L, drain the chloroform and use it as material II for subsequent use;

[0037] 4) Mix material Ⅱ with Schwann cell suspension (cell concentration is 1×10 7 pc / ml) after 3 days of co-cultivation, it will be used as material III for ...

Embodiment 3

[0040]1) Take the skin with an area of ​​10mm (width)×30mm (length) from the patient’s wound, remove the epidermis and subcutaneous tissue, and cut it into a film shape of 10mm (width)×0.5mm (thickness)×30mm (length) , after routine decellularization and freeze-drying, it was used as material I for later use;

[0041] 2) Take a 1 mm long nerve along the broken end from the damaged part of the peripheral nerve of the patient, and obtain Schwann cells by conventional in vitro culture;

[0042] 3) Process material Ⅰ into a fiber shape of 0.2mm (width)×0.2mm (thickness)×30mm (length), soak in ephrin-B2 protein aqueous solution with a concentration of 0.001μg / L for 10min, freeze-dry, and then Soak in the chloroform solution of PLGA with a concentration of 30g / L, drain the chloroform and use it as material II for subsequent use;

[0043] 4) Mix material Ⅱ with Schwann cell suspension (cell concentration is 1×10 6 Individual / ml) after co-cultivation for 3 days, it will be used as m...

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Abstract

The invention discloses a method for preparing a peripheral nerve injury repair promotion stent containing ephrin-B2 protein, which comprises the steps of using the autologous skin of a patient as a material for a neural stent and utilizing nerves at the injured position of autologous peripheral nerves of the patient to obtain Schwann cells used as a material for preparing the neural stent, wherein the material selection mode can control the function damage caused by material selection operation on the patient at a minimum level. The stent has the effect of effective guide on axonal regeneration, and can repair nerve defect with longer distance compared with the prior art. Compared with the repair of ruptured nerves, through the method, the stent is connected between broken ends to promote the repair of missing parts of peripheral nerves based on missing nerves between the broken ends after the peripheral nerves rupture, and the stent is higher in difficulty and clinical application value.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a preparation method of a scaffold containing ephrin-B2 protein for promoting peripheral nerve injury repair. Background technique [0002] Eph is a kind of receptor, which belongs to a subfamily of the receptor tyrosine kinase (RTKs) family, and is a membrane-bound protein that exists on the surface of the cell membrane in a transmembrane form. Ephrin ( Eph family receptor interacting proteins ) is a ligand that binds to Eph, and the combination of the two mediates intercellular signal transduction. Ephrin is divided into two subfamilies, ephrin-A and ephrin-B, among which ephrin-B includes three proteins, namely ephrin-B1, ephrin-B2 and ephrin-B3. [0003] The latest research (Parrinello S et al., Cell. 2010 Oct 1;143(1):145-55.) shows that ephrin-B2 can guide the directional growth of Schwann cells at the site of peripheral nerve injury, and then guide the axon to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61L27/18
Inventor 刘彬张耀光王志坚蒲德永戴丽
Owner SOUTHWEST UNIV
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