Cryopreservation method for adipose tissues

Abstract

The invention belongs to the field of biotechnology, and discloses a cryopreservation method for adipose tissues. The cryopreservation method includes the steps of preparing adipose tissue cryopreservation protection liquid, treating lipoaspirate, cryopreserving the adipose tissues, performing refrigeration according to a pre-controlled freezing curve after a cryopreservation tube containing the adipose tissues is balanced at the temperature of 4 DEG C, and storing the adipose tissues in a liquid nitrogen tank at the temperature of minus 196 DEG C for a long time. According to the characteristics and application features of the adipose tissues, the activity of the cryopreserved adipose tissues is further improved by reducing the damage of the thermal stress to the adipose tissues, controlling the freezing rate and optimizing cryoprotectants in the cryopreservation process of the adipose tissues; the cryopreservation method has the advantages of stable preservation effect, capability ofeffectively improving the activity of the adipose tissues after re-thawing, and ideal transplantation effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cryopreserving adipose tissue. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Since Dr. Coleman published the first report on autologous fat transplantation in 2001, autologous fat transplantation has been widely used in the field of plastic surgery. At present, autologous fat transplantation can be mainly used for rejuvenation of the face and hands, scar repair, breast reconstruction, enlargement and large-scale reconstruction of other parts. In addition, fat can also be a perfect source of materials for applications in tissue engineering and regenerative medicine. For example, people with diabetes, rheumatoid arthritis, the elderly or other people with chronic wounds and ulcers can benefit from this technology. So far, after autologous fat transplantation, the main obstacle affect...

AI Technical Summary

Problems solved by technology

[0008] (1) In addition to the factors of cryopreservation solution during tissue cryopreservation, there are other factors that may affect tissue rethawing activity, which will result in lower yield and viability of fat and adipose-derived mesenchymal stem cells

[0009] (2) The currently commonly used cryopreservation solution contains fetal bovine serum, culture medium and other components. Fetal bovine serum has the possibility of immunogenicity and virus contamination risk. Uncertain components in commonly used commercial medium may also bring certain immunity Originality, these will lead to adverse reactions in the human body in the later clinical application

[0010] (3) In the existing technology, there is no design in the process of adipose tissue cryopreservation to reduce the damage of thermal stress to adipose tissue and control the freezing rate. Consequences such as activity of adipose tissue, unsatisfactory transplant results, etc.

[0011] (4) Due to the uncontrollability of the operation process and the instability of the cryopreservation effect, the existing adipose tissue cryopreservation technology cannot form a standardized cryopreservation plan, nor can it carry out large-scale industrial production in the later stage

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