Alliin antibody prepared through immunization of guinea pigs

An alliin, guinea pig technology, applied in the direction of anti-plant immunoglobulin, serum immunoglobulin, etc., can solve the problem of lack of immune antigen and antibody research.

Active Publication Date: 2018-12-07
ZUNYI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention intends to provide an alliin antibody prepared by immunization of guinea pigs, so as

Method used

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  • Alliin antibody prepared through immunization of guinea pigs
  • Alliin antibody prepared through immunization of guinea pigs

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Experimental program
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Embodiment Construction

[0020] The following is a further detailed description through specific implementations:

[0021] Preparation of antigen

[0022] Preparation of Alliin Antigen: Add 10ml of egg white to 2ml of 100mg / ml alliin solution with a mass concentration of 100mg / ml, stir and mix well, showing the naked eye visible milky white alliin and egg white conjugate precipitation to form alliin antigen.

[0023] Preparation of adjuvant antigen: weigh 5g of lanolin, 20ml of paraffin oil, autoclave for use as a Freund's insufficiency adjuvant, take 10ml of Freund's insufficiency adjuvant, add 60mg of BCG vaccine dropwise under aseptic conditions, while adding Milling is Freund's complete adjuvant; then the same amount of alliin antigen is added dropwise, and the same is added while milling to form a stable suspension to obtain the adjuvant antigen.

[0024] Animal immunity

[0025] Guinea pigs are required to weigh 300-500g. The immunization injections are performed according to Table 1. Pay attention to th...

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Abstract

The invention discloses an alliin antibody prepared through the immunization of guinea pigs. The alliin antibody contains an alliin antigen and a Freund's complete adjuvant, the alliin antigen and theFreund's complete adjuvant with equal amount are mixed to obtain an adjuvant antigen, the alliin antigen is injected into the body of a guinea pig, and the alliin antibody is obtained after the immune response. According to the alliin antibody, the immunogenicity of alliin is solved, the antibody is acquired through the immunization of the guinea pigs, and the alliin antibody can be applied to research of the concentration detection, in-vivo distribution and pharmacological action mechanisms of alliin and has important application values in the foundation and clinical researches of alliin.

Description

Technical field [0001] The invention belongs to the technical field of biological preparations, and particularly relates to an alliinine antibody prepared by immunization of guinea pigs. Background technique [0002] Alliin is a water-soluble amino acid compound of garlic with stable chemical properties. In recent years, in vitro experimental studies have found that alliin has anti-microbial, anti-tumor, anti-oxidant, immune-regulating and cardiovascular effects. No clinical research has been seen so far. Report. [0003] Low-molecular-weight compounds are generally not immunogenic. When combined with high-molecular compounds, they can activate the body's immune cells to produce corresponding antibodies. Alliin is a low-molecular-weight amino acid compound and has no immunogenicity. However, alliin has amino, carboxyl and thioether groups and can bind to protein molecules. Whether the conjugate is immunogenic and stimulates the body to produce antibodies. No related reports. To ...

Claims

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Application Information

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IPC IPC(8): C07K16/16C07K16/06
CPCC07K16/06C07K16/16
Inventor 李明强韩志俊宋姗姗裴天容
Owner ZUNYI MEDICAL UNIVERSITY
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