Alliin antibody prepared through immunization of guinea pigs
An alliin, guinea pig technology, applied in the direction of anti-plant immunoglobulin, serum immunoglobulin, etc., can solve the problem of lack of immune antigen and antibody research.
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[0020] The following is a further detailed description through specific implementations:
[0021] Preparation of antigen
[0022] Preparation of Alliin Antigen: Add 10ml of egg white to 2ml of 100mg / ml alliin solution with a mass concentration of 100mg / ml, stir and mix well, showing the naked eye visible milky white alliin and egg white conjugate precipitation to form alliin antigen.
[0023] Preparation of adjuvant antigen: weigh 5g of lanolin, 20ml of paraffin oil, autoclave for use as a Freund's insufficiency adjuvant, take 10ml of Freund's insufficiency adjuvant, add 60mg of BCG vaccine dropwise under aseptic conditions, while adding Milling is Freund's complete adjuvant; then the same amount of alliin antigen is added dropwise, and the same is added while milling to form a stable suspension to obtain the adjuvant antigen.
[0024] Animal immunity
[0025] Guinea pigs are required to weigh 300-500g. The immunization injections are performed according to Table 1. Pay attention to th...
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