Rice temperature sensitive male sterility gene, coding protein and application thereof

Abstract

The invention relates to a rice temperature sensitive male sterility gene, a coding protein and application thereof. The rice temperature sensitive male sterility gene has one of the following nucleotide sequences, i.e. 1, a nucleotide sequence shown in SEQ IN NO1, and 2, a NDA sequence which is shown in SEQ IN NO3, has highest homology with a base sequence of an arabidopsis gene and an expected value of 1.8e-27 and is used for coding same biological functional proteins. The transcription expression of the gene OsATMS1 has a characteristic of being sensitive to the environment temperature, can ensure that the plant male fertility has a temperature sensitive characteristic, is expected to have an application value on two aspects of crop crossbreeding and yield increasing.

Description

technical field [0001] The invention relates to a temperature-sensitive male sterility gene of rice, its encoded protein and its application. Background technique [0002] Hybrid breeding technology: Male sterility is a very common phenomenon in the plant world, which is controlled and affected by various internal and external factors. Because male sterile varieties provide great convenience for cross-pollination in agricultural production, they become the basis of cross-breeding and production. In the 1970s, a group of rice breeders in my country, represented by Yuan Longping, successively bred restorer lines and maintainer lines on the basis of cytoplasmic male sterile wild lines, and created the so-called three-line method of hybrid breeding (sterile line, Restorer line and maintainer line), so far, 500 million hectares of hybrid rice based on the three-line method have been sown in my country, increasing rice yield by more than 20%, making a huge contribution to my count...

AI Technical Summary

Problems solved by technology

Although the three-line method has made outstanding achievements, its genotype is single, and the cytoplasmic male sterility is derived from defects in the development of mitochondria or chloroplasts. Under certain genetic backgrounds, it may cause hidden dangers in the overall growth and adaptability of plants.

[0003] However, although the conditional male sterile lines sensitive to external environmental factors represented by temperature-sensitive male sterile lines have emerged in application, compared with the original three-line method, because of the unstable characteristics of this type of male sterile , in the case of abnormal low temperature or high temperature, the fertility will recover to a certain extent. Therefore, the use of this type of male sterile series is greatly restricted by climatic conditions, which severely limits the scope of application.

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