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Aptamer of docetaxel, aptamer derivative and use thereof

A nucleic acid aptamer and docetaxel technology, which can be applied to preparations for in vivo tests, medical preparations without active ingredients, and medical preparations containing active ingredients, etc., and can solve the problem of no nucleic acid aptamers being found. , to avoid adverse drug reactions, improve stability, and achieve the effect of suitable drug release speed

Active Publication Date: 2014-07-02
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, no nucleic acid aptamer with high affinity and low cost to docetaxel has been found

Method used

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  • Aptamer of docetaxel, aptamer derivative and use thereof
  • Aptamer of docetaxel, aptamer derivative and use thereof
  • Aptamer of docetaxel, aptamer derivative and use thereof

Examples

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Embodiment

[0035] A nucleic acid aptamer for docetaxel of the present invention, the nucleotide sequence of the nucleic acid aptamer includes a DNA fragment shown in the following sequence, that is, a characteristic sequence capable of specifically binding docetaxel:

[0036] 5’-TTCCAAACGTCCACCGCCAAAA-3’

[0037] (It can also be 5’-AAAACCGCCACCTGCAAACCTT-3’).

[0038] The nucleotide sequence of the aforementioned nucleic acid aptamer is selected from naturally occurring or artificially synthesized sequences, or the same sequence from any other source.

[0039] A nucleic acid aptamer sequence of docetaxel, the sequence of the nucleic acid aptamer contains the same sequence as all the nucleotides of the above-mentioned characteristic sequence.

[0040] A certain position on the nucleotide sequence of the above-mentioned nucleic acid aptamer can be phosphorylated, oxymethylated, methylated, amination, thiolated or isotopelated.

[0041] The nucleotide sequence of the aforementioned nucleic acid aptame...

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Abstract

The invention discloses an aptamer of docetaxel, an aptamer derivative and use thereof. The aptamer sequence of the docetaxel comprises a DNA segment shown in a sequence 1 or a sequence 2. The aptamer can be the derivative obtained from various similar sequences with high homology or the sequence disclosed by the invention. The aptamer and the derivative thereof disclosed by the invention can be used as drug carriers, or applied to drug design and development, drug separation and purification, preparation of drugs and other products, preparation of docetaxel detection probes or target probes and the like. Therefore, the aptamer and the derivative thereof have the advantages that the aptamer and the derivative thereof can be combined with high specificity and high affinity of the docetaxel, can be subjected to chemical synthesis, and is free of immunogenicity, good in biocompatibility, small and stable in molecular weight, easy to store and the like.

Description

Technical field [0001] The present invention relates to a nucleic acid aptamer, a nucleic acid aptamer derivative and its use, and in particular to a nucleic acid aptamer, a nucleic acid aptamer derivative and its use that can be combined with docetaxel. Background technique [0002] Docetaxel is a taxane anti-tumor drug, which mainly interferes with the microtubule network required during cell mitosis and division, and plays an anti-tumor effect. Its adverse reactions are mainly allergic reactions, thrombocytopenia, and tachycardia , Hypotension, neurotoxicity, gastrointestinal reactions and skin toxicity. Docetaxel is low in water solubility. The commercially available drug Taxotere is solubilized with Tween-80, and ethanol is used for solubilization. It will cause side effects such as allergic reactions and hemolytic reactions. It has potential risks in clinical applications. Therefore, how to reduce Taxotere Side effects and improving clinical efficacy have become urgent pro...

Claims

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Application Information

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IPC IPC(8): C12N15/115A61K47/26A61K49/00G01N21/64A61K31/337
Inventor 羊小海王柯敏陈南迪王青朱莹
Owner HUNAN UNIV
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