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Cabbage type rape, parent species Chinese cabbage thereof, cabbage TT2 (Transparent Testa 2) gene family and application thereof

A technology of Brassica napus and gene family, applied in the field of genetic engineering, achieves the effect of restoring seed coat pigment accumulation, good application prospects, and reducing seed coat pigment

Inactive Publication Date: 2011-01-12
SOUTHWEST UNIVERSITY
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Problems solved by technology

However, at present, the number of members, protein characteristics, evolutionary relationship, tissue specificity of expression, relationship with yellow seed traits, and application in genetic engineering of Brassica TT2 genes such as Brassica napus, Chinese cabbage, and Brassica oleracea have not been found. to report

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  • Cabbage type rape, parent species Chinese cabbage thereof, cabbage TT2 (Transparent Testa 2) gene family and application thereof
  • Cabbage type rape, parent species Chinese cabbage thereof, cabbage TT2 (Transparent Testa 2) gene family and application thereof
  • Cabbage type rape, parent species Chinese cabbage thereof, cabbage TT2 (Transparent Testa 2) gene family and application thereof

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[0039] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0040] The plant material that preferred embodiment adopts: the material of cabbage is the black seed line 06K130 and the yellow seed line 06K124 of Chinese cabbage type rape subspecies (B.rapa ssp.oleifera), the material of cabbage is kale variety (B.oleracea var.acephala ) black seed line 06K158 and yellow seed line 06K165, the materials of Brassica napus are typical black seed maintainer line 5B, Zhongyou 821, black / yellow seed near-isogenic lines (black seed line L1, yellow seed line L2), all ...

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Abstract

The invention relates to the technical field of gene engineering, in particular to cabbage type rape, a parent species Chinese cabbage thereof, a cabbage TT2 (Transparent Testa 2) gene family and application thereof. The Chinese cabbage TT2 gene family comprises two members of a BrTT2-1 gene and a BrTT2-2 gene; the cabbage TT2 gene family has one member of a BoTT2 gene; the cabbage type rape TT2 gene family comprises three members of a BnTT2-1 gene, a BnTT2-2 gene and a BnTT2-3 gene; and the six brassica TT2 genes have higher homology. The invention also discloses the application of the gene family in plant molecular breeding. After the antisense inhibition of the expression of the cabbage type rape in black-seed cabbage type rape, a transgenic plant generates the character variation of testa color lightening, and the like and has potential for creating transgenic yellow-seed materials.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to Brassica napus and its parent species Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea) TT2 (TRANSPARENT TESTA 2, transparent seed coat 2; also known as MYB123) gene family and its application. Background technique [0002] Brassica (Brassica) is the most important genus among more than 300 genera in the Brassicaceae (Brassicacease), containing worldwide important vegetables, oilseeds and ornamental crops, such as cabbage (B.rapa), cabbage (B.oleracea) and Brassica napus (B. napus), and Brassica napus is an allotetraploid formed by natural interspecific hybridization and doubling of Chinese cabbage and Brassica napus. Arabidopsis thaliana is the most well-studied model plant from the Brassicaceae family. Chromosomal molecular marker collinearity studies found that there were genome-level and gene-level conservation among Brassica plants and between Bra...

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N5/10A01H5/00
Inventor 柴友荣张迪位运粮杜娟李加纳吕俊唐章林申敏付春
Owner SOUTHWEST UNIVERSITY
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