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Multifunctional MYB transcription factor gene and application thereof

A transcription factor, multifunctional technology, applied in the field of genetic engineering, can solve problems such as ineffective utilization and unknown function

Active Publication Date: 2019-04-05
INST OF ANIMAL SCI CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that the function of ginkgo MYB transcription factor, especially the MYB transcription factor regulating flavonoids is unknown and cannot be effectively utilized, the present invention provides a multifunctional MYB transcription factor gene and its application

Method used

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  • Multifunctional MYB transcription factor gene and application thereof
  • Multifunctional MYB transcription factor gene and application thereof

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Embodiment 1

[0024] Example 1: A multifunctional MYB transcription factor gene discovered in the present invention is denoted as GbMYB2. Its gene sequence is shown in SEQ ID NO.1. ATG is the start codon in the sequence shown in SEQ ID NO.1. TGA is a stop codon, and the amino acid sequence of the protein encoded by this gene is shown in SEQ ID NO.2. Its use is that heterologous expression in other organisms can systematically regulate various compounds, especially flavonoids.

[0025] This gene was identified and cloned in Ginkgo biloba. The following describes how to clone this gene from Ginkgo biloba, confirm that it is a MYB type transcription factor gene, and the method to determine its use:

[0026] 1. Materials and methods:

[0027] 1.1 Plant material

[0028] The ginkgo leaves used in this experiment were taken from a female ginkgo tree (Ginkgobiloba L.) with a diameter of 20 cm in the Beijing Botanical Garden of the Chinese Academy of Sciences.

[0029] 1.2 Reagents

[0030] CTAB, β-mercaptoe...

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Abstract

The invention discloses a multifunctional MYB transcription factor participating in multiple approaches. The MYB type transcription factor gene is GbMYB2 identified from a 'living fossil' plant gingko, wherein the gene sequence of the MYB type transcription factor gene is as shown in SEQ ID NO.1, and the coded protein sequence is as shown in SEQ ID NO.2. In addition, the invention discloses application of the gene. According to the application of the gene, coded protein can regulate and control plant flavonoid, lignin and plant growth and development. The MYB transcription factor gene and theapplication thereof have the advantages that an MYB2 transcription factor gene is cloned from the ginkgo, and system identification is carried out on the functions of the MYB2 transcription factor gene, and therefore it is discovered that the gene participates in a plurality of metabolic pathways and is a multifunctional transcription factor.

Description

Technical field [0001] The invention belongs to the technical field of genetic engineering, and is specifically a multifunctional MYB transcription factor gene and its use. Background technique [0002] Flavonoids are an important secondary metabolite widely present in plants, and are of great significance for plants to adapt to their habitat. In addition, flavonoids have many functions such as anti-oxidation and scavenging free radicals, and have important applications in human health care. Plants are the main source of food and medicine for humans. Therefore, how to effectively regulate the accumulation of flavonoids in plants to help humans obtain better medicinal and food resources has become a research hotspot in the field of plant secondary metabolism. Transcription factors play an important role in many processes of cell development. Therefore, they are important candidate genes for improving bioengineering technology that regulates a series of complex traits of crops. O...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20
CPCC07K14/415C12N15/8243C12N15/8261
Inventor 庞永珍苏小佳夏亚迎江文波
Owner INST OF ANIMAL SCI CAAS
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