67 results about "Plant secondary metabolism" patented technology

The present invention relates to the field of plant secondary metabolites. More particularly plant cell lines are established from the medicinal plant Veratrum californicum. Said plant cell lines can be used for the production of cyclopamine and related steroid alkaloids and their precursors.

The invention discloses a method for inducing bees to pollinate among parents of male sterile line seed, comprising the following steps of: adding 0.05-0.1 part of plant secondary metabolites into syrup for feeding the bees; and feeding bee colonies which pollinates. Feeding at a time each day, wherein, feeding time is from 18:00 to 20:00 PM, and feeding quantity is 100-200 mg / bee colony / day. Feeding is continued until the end of florescence, and the pollinating bee colonies are moved out of a big-arch shelter. The method can stimulate the bee colonies to firstly pay a visit to male parents and carry the pollen of the male parents to pay a visit to female parents, so as to realize the cross pollination among the parents. The induction function has no specificity, namely, any one of compounds has the function for stimulating the bees to crossly pollinate among the parents. The invention is not limited by the cross pollination between special crops and special parents, has simple method, obvious economic benefit and wide application prospect.

The invention relates to an algaecide. The algaecide which can be used for effectively removing algae and precipitating alga bodies contains chitosan, fly ash autoclaved brick powder and allelopathic chemicals of gallic acid, nonoic acid or N-phenyl-naphthylamine, and is obtained by dissolving a certain amount of chitosan by using hydrochloric acid, then adding the fly ash autoclaved brick powder of 40-200 meshes, and then adding the dimethyl sulfoxide assisted dissolved allelopathic chemicals of gallic acid, nonoic acid, N-phenyl-1-naphthylamine or N-phenyl-2-naphthylamine. The chitosan and the fly ash autoclaved brick powder used in the algaecide provided by the invention are nontoxic, the allelopathic chemicals of gallic acid, nonoic acid and the like belong to plant secondary metabolism products, can be degraded easily in a water body and cannot cause secondary pollution, and although the diluted HCl solution is used for dissolving chitosan, great changes of the pH value of the water body are not caused in a dosage range. The algaecide provided by the invention can be used for removing the algae effectively and ensuring that the alga bodies are sunk to the bottom of water, has a small influence on the environment and does not cause secondary pollution, the water body is clear, and the beautiful outlook of the water body is not influenced, so that the algaecide is suitable for removing the algae from a small landscape water body taking blue algae as a dominant species.

The present invention is directed to a method for altering secondary metabolism in plants, specifically phenylpropanoid metabolism. The present invention is further directed to a mutant p-coumarate 3-hydroxylase gene, referred to herein as the ref8 gene, its protein product which can be used to prepare gene constructs and transgenic plants. The gene constructs and transgenic plants are further aspects of the present invention.

The invention discloses a method for producing 4-hydroxycinnamaldehyde by catalyzing a recombinant strain and whole cells thereof. The method is characterized in that 4-hydroxycinnamic acid is catalyzed by using whole cells of a recombinant strain as a biocatalyst to prepare the 4-hydroxycinnamaldehyde, and the method comprises the following steps: (1) constructing a recombinant strain E.coli M15 / pQE31-4CL1-CCR; (2) preparing whole cells of the recombinant strain; and (3) catalyzing the whole cells of the recombinant strain to produce the 4-hydroxycinnamaldehyde, and detecting the obtained product by high performance liquid chromatography-tandem mass spectrometry. The invention provides a path for synthesizing the 4-hydroxycinnamaldehyde by using a bioconversion method for the first time; the method has the advantages of mild reaction conditions, high enzyme activity, cost conservation, convenience in operation and the like; and the invention provides a new method for synthesizing the 4-hydroxycinnamaldehyde, and also provides a new thought for producing secondary metabolites of plants.

The invention relates to an analytical method for related enzymes in a biosynthesis pathway of plant secondary metabolites. Specifically, the present invention provides a compound represented by the formula (I) L-B shown in the specification, wherein L is a linking group substituted by a click chemical functional group and / or a photoaffinity group; and B is a substrate of a functional protein. Theinvention also provides a probe molecule formed by the compound of formula (I) and a reporter group. The invention also provides a method for identifying functional proteins by use of the compound offormula (I) or the probe molecule and use of the compound of formula (I) or the probe molecule. The analytical method is capable of rapidly and specifically labeling the proteins in a functional state in complex biological samples.

An ultrasonic-assisted extraction method for anthocyanin in hibiscus syriacus petals belongs to the field of plant secondary metabolite extracting, and comprises: grinding hibiscus syriacus petals in liquid nitrogen into a powder, adding a mixed solution of hydrochloric acid and methanol, and extracting anthocyanin from hibiscus syriacus petals under the conditions of constant-temperature water bath and ultrasonic environment. The method employs the mixed solution of hydrochloric acid and methanol with a ratio of 0.1% (v / v) as the extraction solvent and is assisted by ultrasonic for extracting anthocyanin from hibiscus syriacus petals, and the method helps to substantially reduce the extraction time of hibiscus syriacus anthocyanin and improve the extraction efficiency.

The invention provides a large-scale metabolome qualitation method. By using a liquid chromatogram-high resolution mass spectrum non-targeting in-source dissociation technique, systematic large-scalequalitation is carried out on plant metabolome by using multiple strategies such as characteristic neutral loss, multiple neutral loss combinations, neutral loss with multi-stage mass spectrums and characteristic daughter ion recognition, laying a base for further study on variation of plant metabolome. Based on the advantages of high-resolution and high-sensitivity mass spectrums, large-scale characteristic structured metabolin is identified, the types of metabolin can be rapidly locked, the qualitative performance of a conventional mass spectrum method is widened, the metabolin qualitation efficiency is improved, the metabolome cover range is widened, particularly the difficulty of qualifying secondary metabolite of plants is partially solved, and meanwhile, reference can be provided forlarge-scale qualitation study of other living bodies.

The invention discloses a natural traditional Chinese herbal medicine capable of inhibiting and touching out cyanobacterial bloom. The natural traditional Chinese herbal medicine comprises the following components in percentage by weight: 10 to 30 percent of coptis chinensis, 10 to 25 percent of radix bupleuri, 5 to 20 percent of rheum officinale, 5 to 15 percent of schisandra chinensis, 5 to 15 percent of kaladana, 5 to 15 percent of sow thistle, 5 to 10 percent of spina gleditsiae, 3 to 8 percent of humifuse euphorbia herb, 4 to 6 percent of ginkgo leaves, 2 to 5 percent of lotus leaves and2 to 5 percent of little groundcherry herb fruit. The natural traditional Chinese herbal medicine is prepared by the following steps: selecting, cleaning and sterilizing the above traditional Chineseherbal medicine raw materials, weighing the traditional Chinese herbal medicine raw materials according to the percentage by weight, drying, chopping and mixing uniformly; adding water to soak, performing heating reflux and taking filtrate; and adding water and performing heating reflux again, discarding residues and taking the filtrate, and mixing the twice filtrate to prepare the traditional Chinese herbal medicine. The principle that plant secondary metabolites (allelopathic chemicals) have remarkable allelopathic inhibition and touch-out effects on algae is utilized, and various traditional Chinese herbal medicines are combined according to the traditional Chinese medicine combination principle, so that the effects of performing low-concentration synergistic inhibition and touch-out onthe algae by the allelopathic chemicals are achieved, and the advantages of high alga inhibiting speed, safety, no toxicity and the like are achieved.

The invention discloses a method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture. The method comprises the steps of (1) batch obtaining of plant bodies, wherein the plant bodies are inoculated in reactor devices for culture after being processed, after culture parameters are set, large-scale culture is carried out, operation of the next step is carried out as needed, and the plant bodies are in the state of plant cells, the state of plant tissue, the state of plant organs, the state of cluster buds and the like through sterile culture; (2) generation and induction of plant secondary metabolites, wherein the obtained plant bodies are generated or induced under a certain culture condition so as to obtain needed secondary metabolites; (3) collection and extraction of plant secondary metabolites, wherein the plant bodies are pinellia ternata seedlings, pinellia ternata tubers are taken out and then processed through the grinding step, the ultrasonic extraction step, the vacuum drying step and the like to obtain ephedrine extractives, detection is carried out through an HPLC method, and the ephedrine content in the obtained pinellia ternate tubers can reach 160.30 microgram per gram.

The invention discloses a natural Chinese herbal medicine capable of killing cyanobacterial bloom. The natural Chinese herbal medicine comprises the following components by weight percent: 10-30 partsof astragalus membranaceus, 10-25% of houttuynia cordata, 5-20% of radix rehmanniae, 5-10% of mirabilite, 5-10% of cortex ailanthic, 2-8% of betel nut and 2-5% of ginkgo. The natural Chinese herbal medicine is prepared according to the following steps: selecting, cleaning and sterilizing the traditional Chinese medicine raw materials and then weighting in parts by weight, drying, cutting and uniformly mixing; adding water for soaking, heating, refluxing and then extracting the filtrate; adding water, heating, refluxing and then removing residue and extracting the filtrate; mixing the two filtrates. The principle of the plant secondary metabolite (allelochemical) having an obvious allelochemical killing effect to alga is utilized; various Chinese herbal medicines have a synergic alga-killing effect through the allelochemical in low concentration on the basis of the principle of traditional Chinese pharmacology compatibility; the natural Chinese herbal medicine has the advantages of high alga-inhibiting speed, safety, no toxicity, and the like, and can be used for controlling the large-area phytoplankton bloom.

The invention discloses a method for continuously obtaining secondary ginseng hairy root metabolites through tissue culture. The method comprises the following steps of (1) obtaining plants in batch, namely treating the plants, then inoculating into a culture reactor device, setting culture parameters, then culturing in batch and performing the next operation as required, wherein the plants refer to vegetable cells, plant tissues, plant organs, cluster buds and the like which are obtained by axenic culture; (2) generating and inducing the secondary metabolites of the plants, namely generating or inducing the obtained plants in certain culture conditions, so as to obtain the required secondary metabolites; (3) collecting and extracting the secondary metabolites of the plants, namely taking out ginseng hairy roots and then measuring the contents of total saponins of panax ginseng and panaxoside Re+Rg according to the Pharmacopoeia of the People's Republic of China (Edition 2010), wherein the content of the total saponins of the panax ginseng in the obtained ginseng hairy roots can reach 6.182%, and the content of the panaxoside Re+Rg can reach 0.3752%.

The invention provides a method for synthesizing 3-O-glucose-based oleanolic acid and cellobiose oleanolic acid by using saccharomyces cerevisiae engineering bacteria, and belongs to the field of bioengineering. The method comprises the following steps: synthesizing a codon optimized P450 cytochrome monooxygenase gene, a cytochrome reductase gene and a UDP-glucosyltransferase gene by a chemical method; constructing corresponding gene expression boxes by combining a saccharomyces cerevisiae promoter with a terminator; constructing gene expression vectors by a DNA (Deoxyribonucleic Acid) klenow fragment assembling method, and importing the gene expression vectors into the saccharomyces cerevisiae capable of producing beta-amyrin. Direct synthesis of the 3-O-glucose-based oleanolic acid and the cellobiose oleanolic acid serving as plant secondary metabolites in the saccharomyces cerevisiae is realized for the first time; in addition, two synthesized compounds can span cytomembrane of the saccharomyces cerevisiae engineering bacteria, and a downstream separation and extraction process is simplified, so that a new idea is provided for producing pentacyclic triterpene compounds with low water solubility and difficulty in spanning membranes by using the saccharomyces cerevisiae. The method is simple in process and can be used for producing the 3-O-glucose-based oleanolic acid and the cellobiose oleanolic acid by fermenting.

The invention belongs to the field of resource utilization of biomass and particularly provides application of a plant secondary metabolite in anaerobic fermentation of biomass. By adding plant secondary metabolite into anaerobic fermentation raw materials of the biomass, microbial activities are promoted, so that the content of methane produced through the anaerobic fermentation of the biomass is increased. The invention is mainly applied to the field of producing methane through the anaerobic fermentation of biomass such as forestry and agricultural waste such as straws, energy crops such as Chinese silvergrass, animal waste, kitchen waste or other solid waste; by adding a small amount of the plant secondary metabolite into an anaerobic fermentation system, the starting of the anaerobic fermentation can be promoted, and the yield of methane can be increased.

The invention relates to the technical field of chemical detection and analysis, in particular to a method for detecting secondary metabolites of plants based on an SWATH to MRM technology. The methodfor detecting the secondary metabolites of the plants based on the SWATH to MRM technology comprises the following steps of (A) sample treatment: adding 25-100mg of taken ground plant samples into acentrifuge tube, adding 500-1500[mu]L of 75% methanol, mixing, centrifuging and taking supernate; (B) machine detection: setting liquid chromatography and mass spectrometry conditions and carrying outmachine detection on the supernate obtained in the step (A); and (C) data processing: processing and analyzing data obtained through machine detection in the step (B). According to the method disclosed by the invention, flux and accuracy of the detection method are further improved by using cooperative interaction of multiple parameters, the matrix effect is reduced, comprehensive and accurate analysis of metabolites is facilitated and the method can be suitable for detection and analysis of the secondary metabolites contained in any tissue and organ of any plant.

The invention provides a plant metabolism regulator and a preparation method thereof and relates to the technical field of fertilizers. The plant metabolism regulator comprises the following components in percentage by mass: 1-70% of composite traditional Chinese herbal medicine extracts, 1-15% of composite immune vitamins, 1-15% of composite macro and medium trace elements, 1-20% of combined nutrients and 20-50% of water-soluble nano organic carbon powder, wherein the composite traditional Chinese herbal medicine extract is prepared by compounding at least two traditional Chinese herbal medicine extracts. The nutrition combination match is balanced, plant secondary metabolism is effectively regulated, and growth and development of plants are promoted. In addition, the invention further relates to a preparation method of the plant metabolism regulator. The preparation method comprises the following steps: mixing the composite traditional Chinese herbal medicine extracts, the compositeimmune vitamins, the composite macro and medium trace elements, the combined nutrients and the water-soluble nano organic carbon powder, thereby obtaining the plant metabolism regulator with balancednutrition and excellent regulation effect.

The invention discloses a method for continuously obtaining plant secondary metabolites by tissue culture. The method comprises the following steps of: (1) obtaining plant materials in batches; (2) generating and inducing the plant secondary metabolites; and (3) collecting and extracting the plant secondary metabolites. The invention further relates to a reactor device for the culture, wherein the upper part of the device uses a layered culture design of plant tissues and organs, so that cultures can be conveniently collected in a staging manner through the design of a multilayer culture rack; the lower part of the device uses a split type design, so that a liquid storage tank can be conveniently dismounted, and desired products can be obtained by collecting culture liquid; and furthermore, the inhibition on the continuous generation by the concentration of the secondary metabolites can be reduced by replacing the culture liquid, so that the secondary metabolites can be continuously obtained. The method and the device provided by the invention have the advantages of large flux, convenience in operation and low cost.

The invention discloses a method for changing the ingredients of plant secondary metabolites by using beta-ocimene, comprising the following steps of: planting or transferring plants or plant tissue cultures in a closed space of which temperature, humidity and light conditions suitable for the growth of the plants or the plant tissue cultures, adding beta-ocimene in the space till the final concentration of the beta-ocimene in the space is 3-40 mu mol / L, and continuously treating the plants or the plant tissue cultures for 1-10 days, wherein the other conditions during the treatment, such as temperature, humidity and light, are suitable for the growth of the plants or the plant tissue cultures, and the specific conditions are different for the different types of plants. Therefore, the constituents and contents of the plant secondary metabolites can be changed through the beta-ocimene, and a new way is provided for obtaining the effective functional ingredients of plants or increasing the contents of the functional ingredients of the plants.

The invention belongs to the field of biotechnology, and particularly relates to a microbial transformation preparation method of hydroxycamptothecin. The invention conducts biotransformation to the camptotheca acuminata cells of a suspension culture by using non-toxic Aspergillus flavus Cr-1 strains (Aspergillus.flavus) and Rhizopus T-34 strains (Rhizopus sp.); the camptotheca acuminate cell cultures after the transformation experience separation and refining such as raw material crushing, reflex extraction, crystallization, silica gel column chromatography, crystallization, and the like, a pure hydroxycamptothecin product is obtained. The invention conducts biotransformation to the camptotheca acuminata cells of the suspension culture by using a microorganism so as to obtain a plant secondary metabolite hydroxycamptothecin which has an anti-tumor activity, wherein, the content of the plant secondary metabolite hydroxycamptothecin achieves 0.4% (g / g) of the dry weight of the cell; a new way of obtaining high-yield hydroxycamptothecin is established, thereby meeting the requirements of sustainable development; the method for separating the hydroxycamptothecin is simple, convenient and feasible; the purity of the product is high; therefore, the invention has good development prospect.

The invention relates to an algaecide. The algaecide which can be used for effectively removing algae and precipitating alga bodies contains chitosan, fly ash autoclaved brick powder and allelopathic chemicals of gallic acid, nonoic acid or N-phenyl-naphthylamine, and is obtained by dissolving a certain amount of chitosan by using hydrochloric acid, then adding the fly ash autoclaved brick powder of 40-200 meshes, and then adding the dimethyl sulfoxide assisted dissolved allelopathic chemicals of gallic acid, nonoic acid, N-phenyl-1-naphthylamine or N-phenyl-2-naphthylamine. The chitosan and the fly ash autoclaved brick powder used in the algaecide provided by the invention are nontoxic, the allelopathic chemicals of gallic acid, nonoic acid and the like belong to plant secondary metabolism products, can be degraded easily in a water body and cannot cause secondary pollution, and although the diluted HCl solution is used for dissolving chitosan, great changes of the pH value of the water body are not caused in a dosage range. The algaecide provided by the invention can be used for removing the algae effectively and ensuring that the alga bodies are sunk to the bottom of water, has a small influence on the environment and does not cause secondary pollution, the water body is clear, and the beautiful outlook of the water body is not influenced, so that the algaecide is suitable for removing the algae from a small landscape water body taking blue algae as a dominant species.

The invention discloses an injurious insect repellent containing hexadecanol and a preparation and using method of the injurious insect repellent. The injurious insect repellent comprises the hexadecanol, and can further be added with auxiliary ingredients such as solvents including ethanol and the like and sodium dodecylbenzene sulfonate. The repellent has obvious repelling effect on injurious insects such as asparagus caterpillar. Moreover, the main ingredient of the repellent is a plant secondary metabolite which can be decomposed in the nature, and thus, the injurious insect repellent is nuisanceless and has wide application prospect.

A parahormone scutellarin promoting plant root elongation, and root elongation effect inhibition of antagonism auxin substances 2,4-D and antagonism methyl jasmonate is provided, and an application of scutellarin in preparation of parahormones for promoting the plant root elongation, and the root elongation effect inhibition of the antagonism auxin substances 2,4-D and the antagonism methyl jasmonate is also provided. The plant root elongation promotion effect of the plant secondary metabolite scutellarin is first disclosed, scutellarin mutually antagonizes with the two phytohormone 2,4-D andmethyl jasmonate having the root elongation inhibition effect, and the antagonism effect is dose-dependent, so the plant root elongation effect of scutellarin is a parahormone effect. Above application is a first application of scutellarin in the plant field, so a new field is displayed for the application of scutellarin, thereby the excavation of the value of scutellarin is promoted.

The invention discloses a pest repellent containing dodecanol and preparation and application methods thereof; the pest repellent comprises dodecanol, and ethanol and other solvents, as well as sodiumdodecyl benzene sulfonate and other auxiliary components. The pest repellent can significantly repel beet armyworm and other pests. In addition, the pest repellent mainly comprises plant secondary metabolites, can degrade in the nature, is a pollution-free pest repellent and has a promoting application prospect.

The invention discloses a method for high-throughput analysis of plant secondary metabolites based on LCMS. A Scheduled-MRM mode is adopted to realize one-needle sample injection within 45min. Meanwhile, 130 types of plant secondary metabolites including anthocyanidin, benzoic acid and derivatives thereof, catechin and derivatives thereof, coumarin and derivatives thereof, dihydrochalcone, flavanone, flavone, flavonol, isoflavone, phenylpropanoids, procyanidine, stilbene, terpenes and the like are subjected to high-throughput absolute quantitative analysis. The method is high in pretreatment extraction efficiency, an instrument analysis method is wide in linear range, and high-abundance and low-abundance secondary metabolites with a large content difference can be detected and analyzed atthe same time; linear correlation is good, and a correlation coefficients are all higher than 0.995; the quantification limit of partial substances is as low as 0.01 ng / mL, and the sensitivity of themethod is high; accuracy is 80.2%-118.2%, and precision is 0.7%-119.9%; and high throughput and method sensitivity can be considered through Scheduled-MRM scanning, and the method can be widely applied to analysis of secondary metabolites in different complex plant samples.

The invention discloses a method for continuously obtaining plant secondary metabolites through tissue culture. The steps of the method include (1) obtaining plant bodies in batches; (2) producing and inducing plant secondary metabolites; and (3) plant secondary metabolites Collection and extraction of raw metabolites. The present invention also relates to a reactor device for cultivation. The upper part of the device adopts the layered culture design of plant tissues and organs, and the harvesting operation of the culture can be conveniently carried out in stages through the design of the multi-layered culture grid; Body design, its liquid storage tank can be easily disassembled, the desired product can be obtained by collecting the culture medium, and the inhibition of the secondary metabolite concentration on its continued production can be reduced through the replacement of the culture medium, so as to ensure the continuous acquisition of secondary metabolites. The invention has the advantages of large throughput, convenient operation and low cost.

The invention discloses a polar auxin transport accelerant which comprises scutellarin or scutellarein which have functions of accelerating polar auxin transport (PAT) and resisting the inhibition function of a PAT inhibitor TIBA to root elongation, and discloses application of scutellarin and scutellarein in preparing the PAT inhibitor, and scutellarein in preparing a root elongation accelerant. The phenomenon that scutellarin and scutellarein are secondary metabolites with an acceleration function on PAT and are plant sourced PAT accelerants are discovered for the first time, scutellarin and scutellarein are used in the field of plants for the first time, a new prospect that effects of secondary metabolites are brought into play in plant physiology is widened, and relatively greatervalues of scutellarin and scutellarein can be made.

The invention provides a seabuckthorn growth regulator. The seabuckthorn growth regulator comprises an auxin analogue indolebutyric acid, a plant secondary metabolites salicylic acid, inorganic calcium salt calcium chloride and an antioxidant vitamin C. The seabuckthorn growth regulator shows a good inductive effect on seabuckthorn cutting rooting, has a certain influence on growth and development of cutting seedlings, and can obviously improve the survival rate of cuttings. From the angle of mass production and use, compared with comparative economy, the seabuckthorn growth regulator has food economic benefits and social benefits and is suitable for popularization and application.

The invention belongs to the field of preparation of plant secondary metabolism components and in particular relates to a coumarin glycoside type compound which is not reported in documents, namely esculetin-5-O-(4'-cis-caffeoyl)-beta-D-glucoside, and a clean and efficient preparation process thereof. The preparation process provided by the invention has the advantages of efficient and clean preparation process, simple and standard operation method, low raw material cost, no damages to product components, high purity and the like.