The present application relates to the field of
tissue culture, in particular to a culture medium combination for culturing
Hibiscus syriacus and application thereof in rapid propagation of
Hibiscus syriacus. The culture medium combination for culturing
Hibiscus syriacus comprises a differentiation medium, an adventitious
bud elongation subculture medium and a strong root culture medium, wherein the differentiation medium comprises differentiation medium I and differentiation medium II, the present application uses part of the differentiation medium in different stages, can fully meet the application required by growth of
callus in different stages, improves
callus differentiation rate and average
bud number, and improves propagation efficiency; the adventitious
bud elongation subculture medium is optimized from the perspective of different basic culture media and different growth hormones, finally guarantees to obtain the best adventitious bud elongation subculture medium, increases effective bud number, guarantees
survival rate, finally selects a specific rooting culture medium, and increases rooting rate. It can be seen that the culture medium combination can improve
propagation rate of
Hibiscus syriacus and guarantee
survival rate of
Hibiscus syriacus.