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Method for separating and extracting composite amino acid from hibiscus syriacus leaf

A technology of compound amino acids and hibiscus leaves is applied in the field of separation and purification of compound amino acids to achieve the effects of saving solvent, improving extraction rate and shortening crushing time.

Active Publication Date: 2014-03-19
BEIJING EURO & AMERICAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are not many research reports on the chemical active components in hibiscus flowers, leaves and seeds, and even less research on the extraction and separation of active components

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The method for separating and extracting compound amino acids from hibiscus leaves comprises the following steps:

[0038] (1) Pretreatment: picking fresh hibiscus leaves, air-drying, drying at 70°C, crushing, passing through a 20-mesh sieve to obtain hibiscus leaf powder;

[0039] (2) Ultrasonic treatment: take the hibiscus leaf powder obtained in step (1) and add water or an organic solvent at a ratio of 1g:15mL of solid to liquid, and ultrasonically treat for 45min at a power of 250W;

[0040] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:120mL, treat at 100rpm at 75°C for 35min, filter, and concentrate the filtrate to 1 / 5 of the original volume under reduced pressure ;

[0041] (4) Impurity removal: Add absolute ethanol to the solution obtained in step (3), the volume ratio of the solution to absolute ethanol is 1:3, filter, and concentrate the filtra...

Embodiment 2

[0047] The method for separating and extracting compound amino acids from hibiscus leaves comprises the following steps:

[0048] (1) Pretreatment: picking fresh hibiscus leaves, air-drying, drying at 70°C, crushing, passing through a 20-mesh sieve to obtain hibiscus leaf powder;

[0049] (2) Ultrasonic treatment: take the hibiscus leaf powder obtained in step (1) and add water or an organic solvent at a ratio of 1g:10mL of solid to liquid, and ultrasonically treat for 40min at a power of 250W;

[0050] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:100mL, treat at 100rpm at 70°C for 30min, filter, and concentrate the filtrate under reduced pressure to 1 / 5 of the original volume ;

[0051] (4) Impurity removal: add absolute ethanol to the solution obtained in step (3), the volume ratio of the solution to absolute ethanol is 1:2, filter, and concentrate the filtra...

Embodiment 3

[0056] The method for separating and extracting compound amino acids from hibiscus leaves comprises the following steps:

[0057] (1) Pretreatment: picking fresh hibiscus leaves, air-drying, drying at 70°C, crushing, passing through a 10-mesh sieve to obtain hibiscus leaf powder;

[0058] (2) Ultrasonic treatment: take the hibiscus leaf powder obtained in step (1) and add water or an organic solvent at a ratio of 1g:20mL of solid to liquid, and ultrasonically treat it for 50min at a power of 250W;

[0059] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:150mL, treat at 100rpm at 80°C for 40min, filter, and concentrate the filtrate under reduced pressure to 1 / 5 of the original volume ;

[0060] (4) Impurity removal: Add absolute ethanol to the solution obtained in step (3), the volume ratio of the solution to absolute ethanol is 1:4, filter, and concentrate the fil...

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PUM

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Abstract

The invention relates to a separation and purification method of composite amino acid, and particularly relates to a method for separating and extracting the composite amino acid from hibiscus syriacus leaves. The method comprises the following steps of: (1) pretreating; (2) ultrasonically treating; (3) decoloring; (4) removing impurities; (5) purifying; (6) dissolving a crystal, adding active carbon, stirring, filtering, placing into a refrigerator for filtration, precipitating an amino acid, and drying to obtain a composite amino acid crystal. The method disclosed by the invention can be used for extracting the composite amino acid from the hibiscus syriacus leaves by jointly acting on the hibiscus syriacus leaves through a complex enzyme and an ultrasonic wave, is moderate in enzyme acting condition and plays a wall-breaking role, namely a role of enabling the amino acid contained in the hibiscus syriacus leaves to be thoroughly dissolved. According to the invention, an ultrasonic wave extracting process is a physical process, no chemical change is generated in the extracting process, and a leached biological active substance is kept unchanged within a short time, so that the crushing time is shortened.

Description

technical field [0001] The invention relates to a method for separating and purifying compound amino acids, in particular to a method for separating and extracting compound amino acids from hibiscus leaves. Background technique [0002] Hibiscus is a deciduous shrub belonging to Malvaceae Hibiscus. It is native to East Asia. It is mainly distributed in the Yangtze River Basin, Yellow River Basin, Pearl River Basin, North Korea, Japan and other countries. Now it is cultivated in temperate and subtropical regions of the world. It is a medicinal and edible herb. , ornamental, greening, and fiber raw material value of woody plants. [0003] The medicinal effects of hibiscus leaves have been recorded in our country. According to "Pinjiang Jingyao", it can control intestinal wind and heat thirst after dysentery. Red and white dysentery, dryness and blockage, and the inability to understand the sinking, pound the juice and drink warm raw white wine. [0004] The young leaves of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C07B63/00
Inventor 张成如
Owner BEIJING EURO & AMERICAN INST OF SCI & TECH
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