Tissue culture regeneration method of Hibiscus hamabo

A hibiscus and tissue culture technology, applied in the biological field, to achieve the effects of high regeneration efficiency, large reproduction coefficient and rich materials

Active Publication Date: 2017-10-20
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the tissue culture of seaside hibiscus is still blank

Method used

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  • Tissue culture regeneration method of Hibiscus hamabo
  • Tissue culture regeneration method of Hibiscus hamabo
  • Tissue culture regeneration method of Hibiscus hamabo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of seashore hibiscus tissue culture regeneration method of the present embodiment is all carried out under sterile conditions, comprising the following steps:

[0029] (1) Select perennial seaside hibiscus plant lines with excellent agronomic traits (with one or more traits in high yield, high quality, and good stress resistance) from the wild, and cut unlignified branches of the current year as the source of explants ;

[0030] (2) After the branches are defoliated, they are cut into 5cm-sized stem segments and subjected to surface disinfection; after surface disinfection, the necrotic parts at both ends of the stem segments are excised, and then the stem segments are cut into at least 0.7 cm long stem segments with at least one axillary bud. Cut into sections with a size of cm and set aside; among them, the method of surface disinfection is: wash the branches of the seaside hibiscus in running water for 20 minutes, wipe them with 75% absolute ethanol, and disin...

Embodiment 2

[0036] A kind of seashore hibiscus tissue culture regeneration method of the present embodiment is all carried out under sterile conditions, comprising the following steps:

[0037] (1) Select perennial seaside hibiscus plant lines with excellent agronomic traits (with one or more traits in high yield, high quality, and good stress resistance) from the wild, and cut unlignified branches of the current year as the source of explants ;

[0038](2) After the branch leaves are removed, it is cut into a stem section of 5cm size, and the surface is sterilized; after surface disinfection, the stem section is cut into at least 0.5cm size cut section with 1 axillary bud, for subsequent use; wherein, the surface The disinfection method is as follows: Rinse the current-year-old branches of the seaside hibiscus with running water for 10 minutes, wipe them with 74% absolute ethanol, and then disinfect them with 74% absolute ethanol for 15 seconds in a sterile operating table; then disinfec...

Embodiment 3

[0044] A kind of seashore hibiscus tissue culture regeneration method of the present embodiment is all carried out under sterile conditions, comprising the following steps:

[0045] (1) Select perennial seaside hibiscus plant lines with excellent agronomic traits (with one or more traits in high yield, high quality, and good stress resistance) from the wild, and cut unlignified branches of the current year as the source of explants ;

[0046] (2) After the branch leaves are removed, it is cut into a stem section of 5 cm in size, and the surface is disinfected; after surface disinfection, the stem section is cut into at least 1.0 cm in length with 1 axillary bud, for subsequent use; wherein, the surface The disinfection method is as follows: Rinse the branches of the seaside hibiscus with running water for 30 minutes, wipe them with 76% absolute ethanol, and then disinfect them with 76% absolute ethanol for 50 seconds in a sterile operating table; then disinfect them with 20% h...

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Abstract

The invention discloses a tissue culture regeneration method of Hibiscus hamabo, comprising the steps of (1) using an underyearling non-lignified shoot of Hibiscus hamabo as an explant source, sterilizing the surface, and cutting the short into segments of suitable size; (2) inoculating the segments to culture media which plant growth regulators of different types and concentrations are added to, and performing sprouting of axillary buds, proliferating, elongating of adventitious buds, and rooting induced culture under suitable lighting and temperature; (3) acquiring regenerated plants of Hibiscus hamabo with strong roots. The method of the invention has the advantages that regeneration efficiency is high, proliferation coefficient is large, good character of female plants can be maintained, basis is laid for rapid large-scale reproduction of good strains of Hibiscus hamabo, and important technical support is also provided for genetically modifying Hibiscus hamabo in later stage by molecular biological means.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for tissue culture and regeneration of hibiscus hibiscus. Background technique [0002] Seaside Hibiscus (Hibiscus hamabo Sieb.et Zucc.) is a small deciduous tree of the Malvaceae Hibiscus genus. It is native to Zhejiang Zhoushan, Ningbo and other coastal areas. After the leaves turn red, the seasonal changes are obvious, and the branches are tough and resistant to pruning. Invasion, also has strong drought resistance, barren resistance and wind resistance, is a kind of protective tree species with great development potential, and plays a very important role in environmental governance and saline-alkali land improvement. [0003] In recent decades, due to various reasons such as land reclamation, man-made damage has been serious, and the seaside hibiscus is on the verge of extinction, and it is listed as a rare and endangered tree species in Zhejiang. At present, seaside h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 侯金艳吴丽芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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