Method for high-throughput analysis of plant secondary metabolites based on LCMS and application

Abstract

The invention discloses a method for high-throughput analysis of plant secondary metabolites based on LCMS. A Scheduled-MRM mode is adopted to realize one-needle sample injection within 45min. Meanwhile, 130 types of plant secondary metabolites including anthocyanidin, benzoic acid and derivatives thereof, catechin and derivatives thereof, coumarin and derivatives thereof, dihydrochalcone, flavanone, flavone, flavonol, isoflavone, phenylpropanoids, procyanidine, stilbene, terpenes and the like are subjected to high-throughput absolute quantitative analysis. The method is high in pretreatment extraction efficiency, an instrument analysis method is wide in linear range, and high-abundance and low-abundance secondary metabolites with a large content difference can be detected and analyzed atthe same time; linear correlation is good, and a correlation coefficients are all higher than 0.995; the quantification limit of partial substances is as low as 0.01 ng / mL, and the sensitivity of themethod is high; accuracy is 80.2%-118.2%, and precision is 0.7%-119.9%; and high throughput and method sensitivity can be considered through Scheduled-MRM scanning, and the method can be widely applied to analysis of secondary metabolites in different complex plant samples.

Description

technical field [0001] The invention belongs to the field of biological analysis, and relates to a method and application for high-throughput analysis of plant secondary metabolites based on LCMS, in particular to an LCMS absolute quantitative analysis method and application of 130 kinds of plant secondary metabolites. Background technique [0002] Plant secondary metabolites (secondary metabolites) refer to a large class of small molecular organic compounds that are not necessary for growth and development in plants, and are natural active ingredients formed during the long-term evolution of plants. At present, the common secondary metabolites include alkaloids, phenylpropanoids, flavonoids, terpenes, saponins, phenolic acids, and quinones, which are important for plant growth, reproduction, adaptation, and other physiological processes. For plants themselves, they have the functions of defense against natural enemies, disease resistance and insect resistance. In addition,...

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Problems solved by technology

[0004] The invention improves the sensitivity and analysis throughput of the detection method by optimizing the LCMS method and the sample pretreatment method, and can detect anthocyanins, benzoic acid and its derivatives, catechin and its derivatives, coumarin and its derivatives, dihydrogen Chalcones, flavanones, flavones, flavonols, isoflavones, phenylpropanoids, proanthocyanidins, stilbenes, terpenes, and a total of 130 phenolic substances in 13 categories were used for high-throughput absolute quantitative analysis. Solve the current analysis problems such as low content of plant secondary metabolites, many isomers, and complex matrix

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