Method for inducing bees to pollinate among parents of male sterile line seed

A honeybee and parent technology, applied in botany equipment and methods, plant genetic improvement, animal feed, etc., can solve the problems of low yield and unsatisfactory effect of hybrid seeds, and achieve shortened effective pollination time, significant economic benefits, Pollination cost reduction effect

Inactive Publication Date: 2009-11-04
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection behavior of bees is very specific, that is, they only visit flowers with the same genetic background during a specific period of time every day, and the genetic backgrounds of male and female parents of male s

Method used

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  • Method for inducing bees to pollinate among parents of male sterile line seed
  • Method for inducing bees to pollinate among parents of male sterile line seed
  • Method for inducing bees to pollinate among parents of male sterile line seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 - Utilizing quercetin to stimulate bees to pollinate cauliflower male sterile lines for hybrid seed production

[0034] 1 material

[0035] The parent is code 3592. The parent was provided by the Economic Crops Research Institute of the Yunnan Academy of Agricultural Sciences, and the female parent is a cytoplasmic male sterile line.

[0036] 2 Experimental design

[0037] The experiment was carried out at the Vegetable Variety Breeding Base in Yuanmou County, Yunnan Province. A plot with an area of ​​1 mu was selected for the experiment, and the planting ratio of the male parent and the female parent was 1:4, which was alternate planting (1 row of duplicates, 4 rows of female parents). The test plots are all isolated from the outside world by gauze greenhouses (3 meters high).

[0038] On February 10, 3 boxes / mu of bees were released, with 8,000 to 10,000 bees per box. One group was fed pure syrup and the other two groups were fed syrup containing querce...

Embodiment 2

[0047] Example 1 was repeated with the following difference: the concentration of quercetin was 0.10%. The results are shown in Table 1 and Table 2.

Embodiment 3

[0049] Repeat Example 1 with the following differences: the secondary metabolite added in the syrup is caffeine, and the concentration of caffeine is 0.05%. The results are shown in Table 1 and Table 2.

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Abstract

The invention discloses a method for inducing bees to pollinate among parents of male sterile line seed, comprising the following steps of: adding 0.05-0.1 part of plant secondary metabolites into syrup for feeding the bees; and feeding bee colonies which pollinates. Feeding at a time each day, wherein, feeding time is from 18:00 to 20:00 PM, and feeding quantity is 100-200 mg/ bee colony/day. Feeding is continued until the end of florescence, and the pollinating bee colonies are moved out of a big-arch shelter. The method can stimulate the bee colonies to firstly pay a visit to male parents and carry the pollen of the male parents to pay a visit to female parents, so as to realize the cross pollination among the parents. The induction function has no specificity, namely, any one of compounds has the function for stimulating the bees to crossly pollinate among the parents. The invention is not limited by the cross pollination between special crops and special parents, has simple method, obvious economic benefit and wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of crop pollination, and relates to a method for inducing bees to cross-pollinate crops. In particular, it relates to a method for inducing honeybees to pollinate between parents for sterile line production. Background technique [0002] my country has a vast territory and superior climatic conditions, and has become the main base for crop seed production. In terms of crop seed production, the seed production technology using male sterile lines has been relatively mature. Many high-yield and high-quality crops have been bred through male sterile lines, which have made great contributions to the development of the world economy. However, in the male sterile line seed production process, the hybridization between the parents is mainly realized by artificial pollination, that is, the pollen of the male parent is collected manually, and the pollen is smeared on the stigma of the female parent. This is a very ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01K67/033A23K1/18A23K1/16A23K20/00A23K20/163A23K50/90
Inventor 刘方邻李建军
Owner XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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