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Polar auxin transport accelerant and application thereof

A technology of plant auxin and accelerator, applied in the direction of plant growth regulator, plant growth regulator, application, etc., can solve the problem of no PAT inhibitor TIBA, etc., and achieve the effect of promoting root elongation

Inactive Publication Date: 2016-10-05
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports in the prior art that baicalein and scutellarein, the aglycone of baicalein, are used as PAT regulators, and there is no report that it can antagonize the PAT inhibitor TIBA on the PAT inhibitory effect

Method used

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  • Polar auxin transport accelerant and application thereof
  • Polar auxin transport accelerant and application thereof
  • Polar auxin transport accelerant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of baicalein:

[0029] Name: Baicalein / Scutellarin / 4-Hydroxybaicalein-7-O-Glucuronide

[0030] (scutellarin / Scutellarein 4'-methyl ether 7-glucuronide)

[0031] Molecular formula: C 21 h 18 o 12

[0032] Structural formula:

[0033]

[0034] Preparation: crushing Erigeron breviscapus (vant) Hand Mass medicinal materials → percolating with 70% ethanol → concentrating the extract → passing the concentrate through a macroporous resin → elution with 50% ethanol → concentrating the eluate → adjusting the concentrate with hydrochloric acid PH value to 1-2 → standing for 24 hours to crystallize → filter the crystals → methanol reflux recrystallization (multiple times) → obtain pure scutellarin. (3g of pure scutellarin obtained from 1kg of Duantingfei Peng medicinal material)

[0035] Preparation of scutellarein:

[0036] Name: Scutellarein / 4',5,6,7-Tetrahydroxyflavone

[0037] (scutellarein / 4',5,6,7-Tetrahydroxyflavone)

[0038] Molecular formula: C 1...

Embodiment 2

[0043] Baicalein and scutellarein promote the expression of auxin polar transport proteins PIN1 and PIN2:

[0044]PIN1 and PIN2 are two main members of the protein family responsible for the polar transport of auxin. PIN1:GFP and PIN2:GFP are respectively transgenic PIN1 fusion reporter gene green in the background of model plant Arabidopsis thalianaecotype Columbia (Col) A mutant strain of the fluorescent protein GFP and PIN2 fused to the reporter gene GFP. Using PIN1:GFP and PIN2:GFP mutant strains as experimental materials, they were cultivated and treated by the method of filter paper culture. Dissolve baicalein and scutellarein powder in DMSO to a final concentration of 0.1mol / L, and then dilute with deionized water to obtain 100 μM baicalein and scutellarein working solution, and dilute DMSO with deionized water in the same proportion for the control solution . Add 100 μM baicalein and scutellarein and the control solution to 9 cm petri dishes, 5 mL per dish, spread th...

Embodiment 3

[0046] Baicalein and scutellarein enhance auxin accumulation at the root tip:

[0047] DR5 is an artificially modified promoter containing auxin response elements, which can reflect the distribution and accumulation of auxin in plants by driving the expression of downstream reporter genes in the presence of auxin. DR5::GUS is derived from Arabidopsis wild-type Col is a mutant strain of the background transgene DR5 fused with the reporter gene GUS. Using the DR5::GUS mutant strain as material, add 100 μM baicalein and scutellarein and the control solution to 9 cm petri dishes, 5 mL per dish, spread three layers of filter paper in the petri dish, sow the seeds evenly on the filter paper, each treatment 3 repetitions. They were vernalized in the dark at 4°C for 2 days, and then transferred to the culture room for cultivation. The culture conditions are photoperiod 14h day / 10h night, day temperature 22°C, night temperature 19°C, light intensity 120μmol·m-2·s-1, humidity 50%-60%....

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Abstract

The invention discloses a polar auxin transport accelerant which comprises scutellarin or scutellarein which have functions of accelerating polar auxin transport (PAT) and resisting the inhibition function of a PAT inhibitor TIBA to root elongation, and discloses application of scutellarin and scutellarein in preparing the PAT inhibitor, and scutellarein in preparing a root elongation accelerant. The phenomenon that scutellarin and scutellarein are secondary metabolites with an acceleration function on PAT and are plant sourced PAT accelerants are discovered for the first time, scutellarin and scutellarein are used in the field of plants for the first time, a new prospect that effects of secondary metabolites are brought into play in plant physiology is widened, and relatively greatervalues of scutellarin and scutellarein can be made.

Description

Technical field: [0001] The invention belongs to the field of plant growth hormones, in particular, it relates to two flavonoids baicalein and scutellarein which promote the polar transport of plant auxin and alleviate the inhibitory effect of inhibitors on the polar transport of plant auxin, and their preparation for plant growth Application in prime polar transport enhancers. Background technique: [0002] Hormone regulation plays a very important role in plant root development (Petricka et al.2012), especially auxin polar transport (polar auxin transport, PAT) is critical for plant root morphogenesis. Auxin is an important hormone that regulates plant growth and development and responds to the external environment, including rooting, flowering, tillering, response to low temperature, and response to salinity, etc., while PAT is a hormone that auxin responds to endogenous and exogenous signals and forms a polar distribution. An important pathway of PAT (Vanneste and Friml...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P21/00
CPCA01N43/16
Inventor 李唯奇张旭东
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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