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Method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture

A technology of secondary metabolites and tissue culture, applied in the field of plant biology, can solve the problems of low throughput and high labor consumption, and achieve the effect of easy enrichment

Active Publication Date: 2014-08-27
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method of plant tissue culture has the disadvantages of low throughput and high labor consumption, and the application of the reactor is a better solution

Method used

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  • Method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture
  • Method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Such as figure 1 , 2 As shown, each part of the device involved in the method for obtaining plant secondary metabolites through plant tissue culture is assembled. The reactor tank cover 3 has threads and is connected to the reactor tank 4. The upper part of the liquid storage tank 8 has threads and is sealed and combined with the lower part of the reactor culture tank 4. The liquid storage tank cover 7 has a The screw thread can be combined with liquid storage tank 8 seals.

[0058] The diameter of the main body of the device is 20cm, and the height of the main body of the device is 30cm; the multi-layer culture grid frame 6 is a material that can withstand high temperature and high pressure (121°C, 30min) for sterilization, and the mesh size is about 0.5cm to support the plant material and allow the culture solution to pass through smoothly. allow. Each layer of the network frame of the multi-layer cultivation network frame 6 utilizes the support of assembling and d...

Embodiment 2

[0073] in accordance with figure 2 As shown in a method for obtaining plant secondary metabolites through plant tissue culture, the various parts of the device involved in the device assembly, the reactor tank cover 3, has a slot to connect with the reactor tank 4, and the liquid storage tank 8 The upper part has a card slot and can be sealed and combined with the lower part of the reactor culture tank 4, and the liquid storage tank cover 7 has a card slot and can be sealed and combined with the liquid storage tank 8;

[0074] The diameter of the main body of the device is 30 cm. The height of the main body of the device is 40cm;

[0075] The multi-layer culture grid is a material that can withstand high temperature and high pressure (121°C, 30 min) sterilization. The mesh size is about 1mm to support the plant material and allow the culture medium to pass smoothly. The grids can be assembled and disassembled by using Supported by pillars, the number of grid layers is 5; th...

Embodiment 3

[0078] in accordance with figure 2 As shown in a method for obtaining plant secondary metabolites through plant tissue culture, the various parts of the device involved in the assembly of the device are the same as described in Example 1, except that the number of grid layers is 2 layers; the grid spacing It is 10cm.

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Abstract

The invention discloses a method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture. The method comprises the steps of (1) batch obtaining of plant bodies, wherein the plant bodies are inoculated in reactor devices for culture after being processed, after culture parameters are set, large-scale culture is carried out, operation of the next step is carried out as needed, and the plant bodies are in the state of plant cells, the state of plant tissue, the state of plant organs, the state of cluster buds and the like through sterile culture; (2) generation and induction of plant secondary metabolites, wherein the obtained plant bodies are generated or induced under a certain culture condition so as to obtain needed secondary metabolites; (3) collection and extraction of plant secondary metabolites, wherein the plant bodies are pinellia ternata seedlings, pinellia ternata tubers are taken out and then processed through the grinding step, the ultrasonic extraction step, the vacuum drying step and the like to obtain ephedrine extractives, detection is carried out through an HPLC method, and the ephedrine content in the obtained pinellia ternate tubers can reach 160.30 microgram per gram.

Description

[0001] The application number is "201310043116.9", the application date is "February 04, 2013", and the title of the invention is "Method and device for continuously obtaining plant secondary metabolites through tissue culture" A divisional application of a Chinese invention patent application . technical field [0002] The invention belongs to the field of plant biotechnology; in particular, it relates to a method for continuously obtaining active ingredients such as plant secondary metabolites through tissue culture and a device for realizing the method. Background technique [0003] Secondary metabolites are non-essential small-molecule organic compounds produced by secondary metabolism in a class of cell life activities or in the normal operation of plant growth and development, and their production and distribution are usually specific to species, organs, tissues, and growth and development stages . It can be divided into seven categories: phenylpropanoids, quinones, f...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈集双贾明良张本厚欧阳平凯金磊磊
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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