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Parahormone promoting plant root elongation and application thereof

A technology for promoting plant roots and hormones, applied in plant growth regulators, plant growth regulators, applications, etc., to achieve the effect of promoting plant root elongation and promoting biological effects

Inactive Publication Date: 2012-08-01
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the application value of scutellarin is reflected in the medical field, but there is no report about its function and use in the field of plants

Method used

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  • Parahormone promoting plant root elongation and application thereof
  • Parahormone promoting plant root elongation and application thereof
  • Parahormone promoting plant root elongation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of scutellarin:

[0037] Name: Scutellarin / 4-Hydroxybaicalein-7-O-Glucuronide

[0038] (scutellarin / Scutellarein 4′-methyl ether 7-glucuronide)

[0039] Molecular formula: C 21 h 18 o 12

[0040] Structural formula:

[0041]

[0042] Preparation: crushing Erigeron breviscapus (vant) Hand Mass medicinal materials → percolating with 70% ethanol → concentrating the extract → passing the concentrate through a macroporous resin → elution with 50% ethanol → concentrating the eluate → adjusting the concentrate with hydrochloric acid PH value to 1-2 → standing for 24 hours to crystallize → filter the crystals → methanol reflux recrystallization (multiple times) → obtain pure scutellarin. (3g of pure scutellarin obtained from 1kg of Duantingfei Peng medicinal material)

Embodiment 2

[0044] Effect of scutellarin on plant root elongation:

[0045] 1. Phenotypic analysis of scutellarin in promoting plant root elongation:

[0046]The model plant Arabidopsis thaliana ecotype Columbia (Col) was used as the experimental material, and it was cultivated and treated by the method of filter paper culture. According to the preparation method in Example 1, the obtained scutellarin powder was dissolved in slightly alkaline deionized water to a final concentration of 0.01mol / L, and then diluted with deionized water to obtain 10, 40, 100, and 400 μM scutellaria B solution. Add scutellarin at various concentrations into 6cm petri dishes, 3mL per dish, and repeat each concentration 5 times (for the control group, the scutellarin solution was replaced with deionized water, and the other operations were the same), and three layers of filter paper were spread in the petri dish. Seeds were evenly sown on filter paper, vernalized in the dark at 4°C for 2 days, and then transf...

Embodiment 3

[0053] Scutellarin antagonizes hormones with root elongation inhibitory effects:

[0054] 1. Antagonism effect of scutellarin and auxin 2,4-D on plant root elongation:

[0055] The root elongation of plants is controlled by multiple factors, and auxin is one of the phytohormones (Pilet and Saugy 1987) with root elongation inhibitory effect, and its effect is opposite to the effect of scutellarin found in step 1 of Example 2 . According to the cultivation and treatment method in Step 1 of Example 2, 100 μM scutellarin was used to combine 10 -9 -10 -4 g / L 2,4-D treated Arabidopsis thaliana, and sampled and measured root length according to the method in step 1 of Example 2. It was found that in 10 -9 -10 -4 Within the concentration range of g / L 2,4-D (auuxin), the effect of scutellarin (100 μM) on promoting root elongation was gradually weakened with the increase of 2,4-D concentration, up to 10 -4 g / L 2,4-D almost completely offset ( Figure 4 ). While the 2,4-D concent...

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Abstract

A parahormone scutellarin promoting plant root elongation, and root elongation effect inhibition of antagonism auxin substances 2,4-D and antagonism methyl jasmonate is provided, and an application of scutellarin in preparation of parahormones for promoting the plant root elongation, and the root elongation effect inhibition of the antagonism auxin substances 2,4-D and the antagonism methyl jasmonate is also provided. The plant root elongation promotion effect of the plant secondary metabolite scutellarin is first disclosed, scutellarin mutually antagonizes with the two phytohormone 2,4-D andmethyl jasmonate having the root elongation inhibition effect, and the antagonism effect is dose-dependent, so the plant root elongation effect of scutellarin is a parahormone effect. Above application is a first application of scutellarin in the plant field, so a new field is displayed for the application of scutellarin, thereby the excavation of the value of scutellarin is promoted.

Description

Technical field: [0001] The invention belongs to the field of plant growth hormones, in particular, it relates to a hormone-like breviscap that promotes plant root elongation, antagonizes the effect of 2,4-D, an auxin substance, on inhibiting root elongation, and antagonizes the effect of methyl jasmonate on inhibiting root elongation. Application of B, and scutellarin in the preparation of promoting plant root elongation, in the preparation of antagonizing auxin-like substances 2,4-D to inhibit root elongation, and in the preparation of hormones antagonizing the effect of methyl jasmonate on inhibiting root elongation . Background technique: [0002] Scutellarin is the main active ingredient in Duantingfei Peng. The current research on the efficacy of scutellarin is mainly used in the treatment of ischemic cardiovascular and cerebrovascular diseases in clinical medicine. This drug can dilate blood vessels and improve blood microcirculation. , reducing vascular resistance a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P21/00
Inventor 李唯奇李艳张旭东陶发清
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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