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Method for detecting secondary metabolites of plants based on SWATH to MRM technology

A technology for secondary metabolites and detection methods, which is applied in the field of plant secondary metabolite detection based on SWATHtoMRM technology, can solve the problems of huge differences in plant metabolite content and huge differences in plant metabolite types, and achieves reduction of matrix effects and dissolution. The effect of increased thrust and improved accuracy

Inactive Publication Date: 2020-01-10
上海阿趣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The content of different plant metabolites varies greatly
[0005] 2. Different types of plant metabolites vary greatly

Method used

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  • Method for detecting secondary metabolites of plants based on SWATH to MRM technology
  • Method for detecting secondary metabolites of plants based on SWATH to MRM technology
  • Method for detecting secondary metabolites of plants based on SWATH to MRM technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Embodiment 1 provides a kind of plant secondary metabolite detection method based on SWATH to MRM technology, comprises the following steps:

[0118] A. Sample processing: Take 50 mg of the ground plant sample and add it to a centrifuge tube, add 1000 μL of 75% methanol, mix and centrifuge, and take the supernatant;

[0119] B, on-machine detection: liquid chromatography and mass spectrometry conditions are set, and the supernatant obtained in step A is carried out on-machine detection;

[0120] C. Data processing: process and analyze the data obtained in step B on-board detection.

[0121] The plant described in step A is a pumpkin leaf.

[0122] The grinding described in step A refers to processing 4min on a 45Hz grinder.

[0123] The mixing described in step A refers to placing the centrifuge tube containing the sample and 75% methanol on a mixer at 4°C overnight (12h).

[0124] The centrifugation described in step A refers to centrifuging the sample at 12500rpm f...

Embodiment 2

[0147] Embodiment 2 provides a kind of plant secondary metabolite detection method based on SWATH to MRM technology, also provides a kind of plant secondary metabolite detection method based on SWATH to MRM technology in detecting the secondary metabolite contained in any tissue organ of plant The specific implementation of the application in the production of metabolites is the same as in Example 1, except that the amount of the plant sample is replaced by 25 mg, and the amount of 75% methanol is replaced by 500 μL.

Embodiment 3

[0149] Embodiment 3 provides a kind of plant secondary metabolite detection method based on SWATH to MRM technology, also provides a kind of plant secondary metabolite detection method based on SWATH to MRM technology in detecting the secondary metabolite contained in any tissue organ of plant The specific implementation of the application in the production of metabolites is the same as in Example 1, except that the amount of the plant sample is replaced by 100 mg, and the amount of 75% methanol is replaced by 1500 μL.

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Abstract

The invention relates to the technical field of chemical detection and analysis, in particular to a method for detecting secondary metabolites of plants based on an SWATH to MRM technology. The methodfor detecting the secondary metabolites of the plants based on the SWATH to MRM technology comprises the following steps of (A) sample treatment: adding 25-100mg of taken ground plant samples into acentrifuge tube, adding 500-1500[mu]L of 75% methanol, mixing, centrifuging and taking supernate; (B) machine detection: setting liquid chromatography and mass spectrometry conditions and carrying outmachine detection on the supernate obtained in the step (A); and (C) data processing: processing and analyzing data obtained through machine detection in the step (B). According to the method disclosed by the invention, flux and accuracy of the detection method are further improved by using cooperative interaction of multiple parameters, the matrix effect is reduced, comprehensive and accurate analysis of metabolites is facilitated and the method can be suitable for detection and analysis of the secondary metabolites contained in any tissue and organ of any plant.

Description

technical field [0001] The invention relates to the technical field of chemical detection and analysis, in particular to a method for detecting plant secondary metabolites based on SWATH to MRM technology. Background technique [0002] There are currently three mainstream technical means for the study of metabolomics: nuclear magnetic resonance (NMR), gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-mass spectrometry (LC-MS). Among them, NMR technology is gradually marginalized because its sensitivity is much worse than that of the latter two. GC-MS and LC-MS occupy the core position of metabolomics with their excellent material separation technology, high sensitivity and high resolution. Especially LC-MS, relying on simple sample pretreatment methods, flexible and wide-ranging chromatographic column selection, and high resolution capability (mass-to-charge ratio can be measured accurately to 4 decimal places), is an important tool for metabolomics re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/72
Inventor 黄强邓军亮黄颖瑜秦欢欢王剑锋王琪杜杰刘志鹏占昌宏钟召赟
Owner 上海阿趣生物科技有限公司
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