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Construction of wild Mus musculus strain c57bl/6. Songjiang 3‑chr1 with chromosome 1 replacement

A chromosome replacement, chromosome technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, animal husbandry, etc., can solve problems such as unclear genetic characteristics

Inactive Publication Date: 2017-11-10
上海西普尔-必凯实验动物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few studies on Songjiang 3 mice, and its genetic characteristics are relatively unclear

Method used

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  • Construction of wild Mus musculus strain c57bl/6. Songjiang 3‑chr1 with chromosome 1 replacement
  • Construction of wild Mus musculus strain c57bl/6. Songjiang 3‑chr1 with chromosome 1 replacement
  • Construction of wild Mus musculus strain c57bl/6. Songjiang 3‑chr1 with chromosome 1 replacement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1, the construction method of chromosome replacement mouse

[0081] The embryos of Songjiang 3 mice were obtained from Shanghai Slack Experimental Animal Co., Ltd., and the cryovial containing the frozen embryos was taken out of the liquid nitrogen; after opening the cap, after 30 seconds at room temperature, add preheated 0.25M sucrose solution, and pipette carefully for 10 seconds. First, move the mixture to a petri dish, then suck out the embryos with an egg washing needle, put them into the preheated M2 solution, let them rest for a while, wash them with M2 solution 5 times, transfer them into M16 solution, and cultivate them in the incubator for use. The embryos are transplanted into the uterus of the mother mouse and develop into mice of the same genotype as the frozen embryos.

[0082] Take Songjiang 3 (obtained from the wild Mus musculus in Songjiang, Shanghai) and C57BL / 6 adult mice, and use Songjiang 3 as the male parent and C57BL / 6 as the female pa...

Embodiment 2、1

[0084] Example 2, No. 1 chromosome genome identification

[0085] 1. Primary Screening and Identification of Chromosome 1 Genome

[0086] Comparing the gene sequences of chromosome 1 of the two strains of Songjiang 3 and C57BL / 6 (refer to the public information of MGI (http: / / www.informatics.jax.org / ) for the sequence), select representative, two Different SNP sites of different strains, these SNP sites are evenly distributed on chromosome 1, and are easy to design and operate. Then design primers for these SNP sites to amplify sequences with characteristic SNP sites, and design the range of PCR amplified fragments between 100 and 400 bp. .mit.edu / primer3 / ) and the Oligo6.0 program (Molecular Biology Insights Inc., USA) were completed. Site-specific probes were designed for these SNP sites, and the length of the LDR probe ligation product was 80-130 bp.

[0087] The chromosomal SNP loci for Songjiang 3 and C57BL / 6 mice are shown in Table 1.

[0088] Table 1, selected SNP s...

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Abstract

The invention relates to an establishment of wild house mouse C57BL / 6. Songjiang 3-Chrl with chromosome 1 substituted. The invention constructs a new chromosome C57BL / 6 substituted laboratory mice strain, and the genetic background of the mice strain mainly employs C57BL / 6 mice, wherein, chromosome 1 mainly employs Songjiang 3 mice. The chromosome substitution strain is used for researching character differences brought by different chromosome genome 1 under the same genome background, and is a useful tool for researching chromosome genome functions using genetic method.

Description

technical field [0001] The invention belongs to the field of mouse strain construction, and in particular relates to the construction of No. 1 chromosome replacement wild Mus musculus C57BL / 6. Songjiang 3-Chr1. Background technique [0002] The lack of genetic diversity in experimental Mus musculus strains will cause great obstacles to the study of complex traits. This is mainly reflected in two aspects: first, the number of alleles at each locus in the population is small, and the number of alleles for polygenic related traits is relatively small. During the research, due to the similarity of the genetic background, the information of some trait-related genes will be missed; secondly, the linkage disequilibrium of the genome is relatively large, which is beneficial to the initial positioning of the trait-related genes, but it causes great difficulties for fine positioning. Resulting in consuming a lot of time and individuals. [0003] The living environment of wild Mus mus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCA01K67/027C12Q1/6888C12Q2600/156
Inventor 赵莹肖君华邢正弘陈国强周宇荀李凯赵丽亚晁天柱柏熊高捷
Owner 上海西普尔-必凯实验动物有限公司
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