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Method, primer set and application thereof for monitoring genetic quality of dba inbred mice

A primer set, inbred line technology, applied in recombinant DNA technology, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of low sensitivity, limited reaction genetic profile, low accuracy, etc. Effect

Active Publication Date: 2022-06-03
GEMPHARMATECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this method for detection has disadvantages such as low accuracy, low sensitivity, limited detection sites, and limited response genetic profile

Method used

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  • Method, primer set and application thereof for monitoring genetic quality of dba inbred mice
  • Method, primer set and application thereof for monitoring genetic quality of dba inbred mice
  • Method, primer set and application thereof for monitoring genetic quality of dba inbred mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A method for monitoring the genetic quality of DBA inbred mice. It includes the use of primers to detect 96 as shown in Table 5.

[0062]

[0063]

[0064]

[0065] The sequences of primer combination 1 to primer combination 96 for detecting 96 SNP sites are specifically shown in Table 6.

[0067]

[0068]

[0069]

[0070]

[0071]

[0072]

[0073] Specifically, the KASP method was used to perform PCR amplification on the sample to be treated with a primer set. The reaction system of KASP method is:

[0076]

[0077]

[0078]

[0082]

Embodiment 2

[0084] The method provided in Example 1 is used to genotype the DBA inbred mice.

[0088]

[0089]

[0090]

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Abstract

The invention relates to the field of genetic background identification and genetic pollution detection of inbred mouse strains, and provides a method for monitoring the genetic quality of DBA inbred mice, a primer set and applications thereof. Specifically, the method includes detecting The genotype of the specific SNP site of the mouse can quickly and efficiently identify DBA strains from related inbred strains, so as to quickly and efficiently distinguish the genetic information of DBA strains from other strains, and realize hybridization in genetic quality monitoring This method has the advantage of being able to monitor the genetic quality of DBA inbred mice at low cost.

Description

Methods, primer sets and applications for monitoring genetic quality of DBA inbred mice technical field The present invention relates to inbred mouse strain genetic background identification and genetic pollution detection field, specifically, relate to Methods, primer sets and applications for monitoring the genetic quality of DBA inbred mice. Background technique Laboratory animal quality control is the core problem of the healthy development of the laboratory animal industry, and the quality control of mouse microorganisms and Genetic background control is an important control factor. The experimental animal strain refers to the source is clear, and adopts a certain mating method to breed, and has similar appearance, unique The biological characteristics and stable genetic characteristics can be used in animal populations for different experimental purposes, with a clear genetic background and Genetically restricted animals under strict genetic control. Accordi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2535/125C12Q2563/107
Inventor 赵静琚存祥马秀英杨旭乐裴喆李永兵陶然张冬冬罗天同侯欢欢张明坤
Owner GEMPHARMATECH CO LTD
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