Construction method of mouse xerophthalmia model induced by concanavalin A and application

Abstract

The invention provides a construction method of a concanavalin A induced mouse xerophthalmia model and application. The construction method of the mouse xerophthalmia model induced by the concanavalin A comprises the following steps that 10 microgram / milliliter of a PBS solution of the concanavalin A is prepared, the PBS solution is sub-packaged into 20 microliter / drop, one drop is sucked by using an insulin injection needle when the PBS solution is used, and the drop is injected into a lacrimal gland on one side of a mouse, the used mouse strain is a common BALB / c mouse, the price is lower, and the model forming rate is 70% or above. The method is a single injection method, the medicine utilization rate is low, the molding period is short, the xerophthalmia model induced by the method accords with part of clinical characteristics of the xerophthalmia, and the xerophthalmia mouse model can be applied to research and development of medicines for treating xerophthalmia.

Description

Technical field [0001] The present invention belongs to the construction of an animal experiment model, which relates to the construction method and application of a mouse dry eye model induced by a knobs. Background technique [0002] Dry eye, that is, corneal drying, is a tear mass or abnormality and dynamic abnormality due to some reasons, thereby making the tear film unstable, accompanied by eye surface inflammation, leading to an eye discomfort or even visual impairment disease. The dry eye animal model currently used can be divided into five categories, including lack of lack of dry eye, evaporate over strong dry eye, mucin lack of dry eye, tears dynamics is often dry eye syndrome, mixed dry Optimism. Includes animal models and drug-induced models using primary sex and surgery models. [0003] The primary animal model is often the most attractive clinical features, but this type is often complex, or the mold is low; the surgical model generally takes the lacrimal gland remo...

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Problems solved by technology

[0003] Although the primary animal model is often the most suitable for clinical features, the preparation of this type of model is often complicated, or the modeling rate is low; the surgical model generally adopts the method of lacrimal gland removal, but the existence of animal lacrimal gland compensation makes the modeling rate lower. Often not high, and the stability of the model is not enough; the drug-induced model is generally considered as the preferred modeling method in the laboratory, including the use of scopolamine to damage the lacrimal gland, the use of androgen receptor antagonists, etc., or to disturb the meibomian gland to affect the tear film of animals damage the lipid layer of the animal, or use vitamin A deficiency to affect the expression of animal goblet cells and mucin, or use light stimulation to make animal tear dynamics abnormal

[0004] Although animal models are widely used at present, there is no ideal model as a research tool

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