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Mouse cryopreserved sperm resuscitation liquid compound and preparation method and application thereof

A preparation method and a technology for resuscitation liquid, which are applied in the comprehensive field of zoology and experimental zoology, can solve the problems of reduced vitality, injury, and reduced fertilization ability, and achieve the effects of enhancing exercise ability, improving fertilization rate, and increasing the probability of fertilization with eggs.

Inactive Publication Date: 2019-01-04
EAST CHINA NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the case of natural mating of mice, the sperm need to go through the reproductive tract of the female mouse to reach the end of the fallopian tube. Various active substances in the reproductive tract interact with the sperm to make it hyperactivated and have the ability to fertilize. This process is called sperm capacitation; the existing frozen In the in vitro fertilization technique of thawed sperm, in vitro fertilization fluid (HTF) is often used to recover sperm, and after recovery, the cryopreserved mouse sperm has reduced viability and fertilization ability due to the damage of cryopreservation and recovery operations. Therefore, how to Improving the fertilization rate of frozen-thawed mouse sperm is a difficult problem to be solved in the process of germplasm resource preservation

Method used

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  • Mouse cryopreserved sperm resuscitation liquid compound and preparation method and application thereof
  • Mouse cryopreserved sperm resuscitation liquid compound and preparation method and application thereof
  • Mouse cryopreserved sperm resuscitation liquid compound and preparation method and application thereof

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Embodiment 1-8

[0045] The sperm resuscitating liquid proposed by the present invention, that is, the composition composed of base liquid, MBCD and PVA is used as the sperm resuscitating liquid. Further, the sperm recovery solution may also include ATP.

[0046] The base fluid, MBCD, PVA and ATP were mixed according to the concentrations of Examples 1-8 to form sperm recovery fluid and fertilization fluid, and the following fertilization rates were obtained, see Table 5.

[0047] Table 5. The preparation situation of mouse sperm resuscitating liquid and semen solution in embodiment 1-8

[0048]

[0049]

[0050] From the above results, it can be seen that compared with Comparative Examples 1-13 and Comparative Example 14, after thawing the frozen mouse sperm, after incubation with the sperm resuscitating fluid complex containing a certain dose of MBCD or PVA of the present invention, a higher concentration was obtained. fertilization rate; use the sperm resuscitation liquid compound co...

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Abstract

The invention discloses a mouse cryopreserved sperm resuscitation liquid compound and a preparation method and application thereof. The compound comprises a basis solution, methyl B cyclodextrin MBCDand polyvinyl alcohol PVA. The preparation method comprises the steps that firstly, methyl B cyclodextrin MBCD and polyvinyl alcohol PVA are dissolved in the basic solution, after sufficient dissolution, the mixture is filtered through a bacterium filter device, and mouse cryopreserved sperm resuscitation liquid is obtained. According to the compound, methyl B cyclodextrin MBCD and polyvinyl alcohol PVA are added to the sperm resuscitation liquid, the sperm capacitation process is promoted, the capacitation of sperms is improved, the number of the sperms with fertilization capacity is increased, the fertilization probability of the sperms and eggs is increased, and the fertilization rate is increased. The compound is beneficial to successful preservation of mouse sperm resources, and reestablishment of mouse strain resources is increased and accelerated.

Description

technical field [0001] The invention belongs to the comprehensive field of zoology and experimental zoology, and in particular relates to a mouse cryopreserved sperm resuscitation liquid compound and its preparation method and application. Background technique [0002] Mouse sperm cryopreservation is currently the most economical and effective way to preserve genetically engineered mouse strains. The in vitro fertilization rate of some closed populations and hybrid F1 generation mice after cryopreservation and recovery is high, which can achieve the purpose of strain reconstruction, but C57BL The in vitro fertilization rate of / 6 frozen-thawed sperm is low, and this strain is the main background strain of genetically engineered mice. Therefore, it is very important to improve the in vitro fertilization rate of genetically engineered mouse strains after frozen-thawed sperm. [0003] In the case of natural mating of mice, the sperm need to go through the reproductive tract of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
CPCC12N5/061C12N2500/50C12N2501/90
Inventor 马雪云
Owner EAST CHINA NORMAL UNIV
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