A compound essential oil for relieving primary dysmenorrhea and preparation method thereof

Abstract

The invention discloses a compound essential oil for relieving primary dysmenorrhea and a preparation method thereof. The compound essential oil for relieving primary dysmenorrhea includes limonene, gingerol, α-terpinene acetate, β-sesquiphellandrene, eucalyptol, p-cymene, β-phellandrene, γ-terpinene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, myrcene, linalool, cis-11-eicosenoic acid, 3-thujacene, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, β-elemene. The present invention has a significant effect of relieving primary dysmenorrhea, and has good safety. In addition, essential oil preparations are easier to penetrate into the skin than cream preparations, and are more likely to activate cells after being absorbed by the skin.

Description

Technical Field

[0001] The invention relates to the technical field of medicines, in particular to a compound essential oil for relieving primary dysmenorrhea and a preparation method thereof. Background Art

[0002] Primary dysmenorrhea refers to abdominal pain during menstruation without other pelvic lesions, accounting for more than 90% of dysmenorrhea. It is related to the increase in the levels of local prostaglandins, leukotrienes, vasopressin, oxytocin and other substances before and after menstruation. It is more common in young women and usually occurs at the menarche or a few months after the menarche. The pain is often spasmodic, located above the pubic symphysis in the lower abdomen, and can radiate to the lumbosacral region and the inner thigh. Severe pain may manifest as pale complexion, cold sweats, nausea, vomiting, diarrhea, etc., and may even affect life and work.

[0003] Generally speaking, mild discomfort caused by primary dysmenorrhea can be relieved by maintaining a good mood and adhering to good living habits; when the pain is unbearable, acetaminophen, ibuprofen, atropine and other drugs can be taken orally (acetaminophen and ibuprofen are prostaglandin synthetase inhibitors, and atropine is an antispasmodic analgesic) to relieve it. However, prostaglandin synthetase inhibitors such as acetaminophen and ibuprofen may cause gastric mucosal damage, and atropine may cause side effects such as dry mouth, facial redness, increased intraocular pressure, and increased heart rate. Therefore, a safer and more effective drug to relieve primary dysmenorrhea is needed. Summary of the invention

[0004] The purpose of the present invention is to provide a compound essential oil for relieving primary dysmenorrhea and a preparation method thereof. The present invention has a significant effect of relieving primary dysmenorrhea and has good safety.

[0005] The technical scheme of the present invention is as follows: a compound essential oil for relieving primary dysmenorrhea, comprising the following components in parts by weight: 1-20 parts of limonene, 1-5 parts of gingerol, 0.1-2 parts of α-pinene acetate, 0.1-2 parts of β-sesquiphellandrene, 0.1-2 parts of eucalyptol, 0.1-0.5 parts of p-cymene, 0.1-0.5 parts of β-phellandrene, 0.1-1 parts of γ-pinene, 0.1-1 parts of α-pinene, 0.1-2 parts of β-bisabolene, 0.1-1 parts of α-curcumene, 0.1-1 parts of trans- 0.1-1 part of caryophyllene, 0.1-1 part of camphene, 0.1-1 part of β-pinene, 0.1-0.5 part of myrcene, 0.1-1 part of linalool, 0.01-0.1 part of cis-11-eicosenoic acid, 0.01-0.1 part of 3-thujacene, 0.01-0.1 part of α-farnesene, 0.01-1 part of sabinene, 0.01-0.1 part of 3-carene, 0.01-0.1 part of nonanoic acid, 0.01-0.1 part of α-copaene, and 0.01-0.1 part of β-elemene.

[0006] The aforementioned compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 2-10 parts of limonene, 1-3 parts of gingerol, 0.1-1 parts of α-pinene acetate, 0.2-1 parts of β-sesquiphellandrene, 0.2-1.5 parts of eucalyptol, 0.2-0.4 parts of p-cymene, 0.3-0.5 parts of β-phellandrene, 0.2-0.8 parts of γ-pinene, 0.1-0.7 parts of α-pinene, 0.1-1 parts of β-bisabolene, 0.1-0.5 parts of α-curcumene, 0.1-0.5 parts of trans-caryophyllene. 2-0.8 parts, camphene 0.1-0.5 parts, β-pinene 0.1-0.5 parts, myrcene 0.1-0.3 parts, linalool 0.1-0.5 parts, cis-11-eicosenoic acid 0.05-0.1 parts, 3-thujene 0.05-0.1 parts, α-farnesene 0.05-0.5 parts, sabinene 0.05-1 parts, 3-carene 0.05-0.1 parts, nonanoic acid 0.01-0.05 parts, α-copaene 0.03-0.15 parts, β-elemene 0.02-0.08 parts.

[0007] The aforementioned compound essential oil for relieving primary dysmenorrhea includes the following components in parts by weight: 4 parts of limonene, 1 part of gingerol, 0.4 parts of α-pinene acetate, 0.4 parts of β-sesquiphellandrene, 0.3 parts of eucalyptol, 0.3 parts of p-cymene, 0.3 parts of β-phellandrene, 0.3 parts of γ-pinene, 0.2 parts of β-bisabolene, and 0.2 parts of α-curcumene. , 0.2 parts of trans-caryophyllene, 0.2 parts of camphene, 0.1 parts of β-pinene, 0.1 parts of myrcene, 0.1 parts of linalool, 0.1 parts of cis-11-eicosenoic acid, 0.09 parts of 3-thujacene, 0.08 parts of α-farnesene, 0.08 parts of sabinene, 0.07 parts of 3-carene, 0.04 parts of nonanoic acid, 0.04 parts of α-copaene, and 0.03 parts of β-elemene.

[0008] The aforementioned compound essential oil for relieving primary dysmenorrhea also includes vegetable oil, which includes the following components in parts by weight: 25-45 parts of oleic acid, 1-30 parts of caprylic acid, 5-25 parts of capric acid, 5-20 parts of linoleic acid, 1-10 parts of palmitic acid, 1-5 parts of stearic acid, 0.1-0.5 parts of cis-11,14-eicosadienoic acid, and 0.1-1 parts of palmitoleic acid. The compound essential oil for relieving primary dysmenorrhea is an external preparation.

[0009] The aforementioned compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 2-10 parts of limonene, 1-3 parts of gingerol, 0.1-1 parts of α-pinene acetate, 0.2-1 parts of β-sesquiphellandrene, 0.2-1.5 parts of eucalyptol, 0.2-0.4 parts of p-cymene, 0.3-0.5 parts of β-phellandrene, 0.2-0.8 parts of γ-pinene, 0.1-0.7 parts of α-pinene, 0.1-1 parts of β-bisabolene, 0.1-0.5 parts of α-curcumene, 0.2-0.8 parts of trans-caryophyllene, 0.1-0.5 parts of camphene, 0.1-0.5 parts of β-pinene, 0.1-0.5 parts of myrcene, 0.1-0.3 parts of camphor 0.1-0.5 parts of alcohol, 0.05-0.1 parts of cis-11-eicosenoic acid, 0.05-0.1 parts of 3-thujane, 0.05-0.1 parts of α-farnesene, 0.05-1 parts of sabinene, 0.05-0.1 parts of 3-carene, 0.01-0.05 parts of nonanoic acid, 0.03-0.15 parts of α-copaene, 0.02-0.08 parts of β-elemene, 30-40 parts of oleic acid, 5-25 parts of caprylic acid, 10-20 parts of capric acid, 10-15 parts of linoleic acid, 3-5 parts of palmitic acid, 1-3 parts of stearic acid, 0.1-0.3 parts of cis-11,14-eicosadienoic acid, and 0.1-0.5 parts of palmitoleic acid.

[0010] The aforementioned compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 4 parts of limonene, 1 part of gingerol, 0.4 parts of α-terpinene acetate, 0.4 parts of β-sesquiphellandrene, 0.3 parts of eucalyptol, 0.3 parts of p-cymene, 0.3 parts of β-phellandrene, 0.3 parts of γ-terpinene, 0.2 parts of α-pinene, 0.2 parts of β-bisabolene, 0.2 parts of α-curcumene, 0.2 parts of trans-caryophyllene, 0.2 parts of camphene, 0.1 parts of β-pinene, 0. 1 part, linalool 0.1 part, cis-11-eicosenoic acid 0.1 part, 3-thujacene 0.09 part, α-farnesene 0.08 part, sabinene 0.08 part, 3-carene 0.07 part, nonanoic acid 0.04 part, α-copaene 0.04 part, β-elemene 0.03 part, oleic acid 30 parts, caprylic acid 21 parts, capric acid 17 parts, linoleic acid 14 parts, palmitic acid 5 parts, stearic acid 1 part, cis-11,14-eicosadienoic acid 0.3 parts, palmitoleic acid 0.1 part.

[0011] The preparation method of the aforementioned compound essential oil for relieving primary dysmenorrhea comprises the following steps:

[0012] A. Wash 1-2 parts of green mandarin peel by weight, extract it by cold pressing method, and obtain 3-thujene, limonene, γ-terpinene, p-cymene, and myrcene;

[0013] B. By weight, 1-2 parts of cardamom, 1-2 parts of bamboo-leaf prickly ash, 1-2 parts of black pepper fruit, 1-2 parts of cinnamon bark, 1-2 parts of ginger rhizomes, and 1-2 parts of chaste tree plants are crushed and mixed evenly, and extracted by steam distillation to obtain gingerol, α-pinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, and β-elemene;

[0014] C, 3-thujacene, limonene, γ-terpinene, p-cymene, myrcene, gingerene, α-terpinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, β-elemene are uniformly mixed to obtain a finished product. The cold pressing method protects the effective ingredients and natural fragrance of plant essential oils to the greatest extent, maximizes the use value of essential oils, and avoids the problems of chemical composition denaturation of essential oils and solvent residues caused by high temperature caused by hot pressing or solvent extraction.

[0015] In the aforementioned preparation method of the compound essential oil for relieving primary dysmenorrhea, in step A, the pH value of the cold pressing extraction is 6-8 and the temperature is 3-7°C; in step B, the temperature of the steam distillation extraction is 28-32°C and the solid-liquid ratio is (4-6):1.

[0016] The preparation method of the aforementioned compound essential oil for relieving primary dysmenorrhea comprises the following steps:

[0017] A. Wash 1-2 parts of green mandarin peel by weight, extract it by cold pressing method, and obtain 3-thujene, limonene, γ-terpinene, p-cymene, and myrcene;

[0018] B. By weight, 1-2 parts of cardamom, 1-2 parts of bamboo-leaf prickly ash, 1-2 parts of black pepper fruit, 1-2 parts of cinnamon bark, 1-2 parts of ginger rhizomes, and 1-2 parts of chaste tree plants are crushed and mixed evenly, and extracted by steam distillation to obtain gingerol, α-pinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, and β-elemene;

[0019] C. Wash 1-2 parts of coconut pulp by weight, extract coconut oil by cold pressing, and extract by fractionation after hydrolysis to obtain caprylic acid and capric acid;

[0020] D. Wash 1-2 parts of sweet almond kernels and 1-2 parts of black cumin seeds by weight, extract them by cold pressing method, and obtain oleic acid, linoleic acid, palmitic acid, stearic acid, palmitoleic acid, and cis-11,14-eicosadienoic acid;

[0021] E. Mix 3-thujacene, limonene, γ-terpinene, p-cymene, myrcene, gingerol, α-terpinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, β-elemene, caprylic acid, capric acid, oleic acid, linoleic acid, palmitic acid, stearic acid, palmitoleic acid, and cis-11,14-eicosadienoic acid in parts by weight.

[0022] In the aforementioned preparation method of the compound essential oil for relieving primary dysmenorrhea, in step A, the pH value of the cold pressing extraction is 6-8 and the temperature is 3-7°C; in step B, the temperature of the steam distillation extraction is 28-32°C and the solid-liquid ratio is (4-6):1; in step C, the number of fractionation plates is 25 and the reflux index is 2-9; in step D, the pH value of the cold pressing extraction is 6-8 and the temperature is 3-7°C.

[0023] Compared with the prior art, the components in the compound essential oil for relieving primary dysmenorrhea in the present invention act synergistically, and it has a significant improvement effect on primary dysmenorrhea; and it can effectively relieve the accompanying symptoms such as fatigue, irritability, diarrhea, anxiety, nausea, dizziness, vomiting, headache, etc. caused by primary dysmenorrhea. The compound essential oil for relieving primary dysmenorrhea in the present invention is administered in the form of external use, which can avoid adverse reactions that may be caused by oral administration, has good safety, and is convenient and simple to use. In addition, essential oil preparations are easier to penetrate into the skin than cream preparations, and are easier to activate cells after being absorbed by the skin. The present invention has a significant effect of relieving primary dysmenorrhea and has good safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is the TIC spectrum of the compound essential oil used to relieve primary dysmenorrhea in Example 2;

[0025] Figure 2 This is a diagram of the accompanying symptoms of the test population before using the compound essential oil for relieving primary dysmenorrhea;

[0026] Figure 3 This is a graph showing the improvement of fatigue symptoms in the test group after using the compound essential oil for relieving primary dysmenorrhea;

[0027] Figure 4 This is a graph showing the improvement of irritability symptoms in the test group after using the compound essential oil for relieving primary dysmenorrhea;

[0028] Figure 5 This is a graph showing the improvement of diarrhea symptoms in the test group after using the compound essential oil for relieving primary dysmenorrhea;

[0029] Figure 6 This is a graph showing the improvement of anxiety symptoms in the test group after using the compound essential oil for relieving primary dysmenorrhea;

[0030] Figure 7 This is a graph showing the improvement of nausea symptoms in the test group after using the compound essential oil for relieving primary dysmenorrhea;

[0031] Figure 8 This is a graph showing the improvement of dizziness symptoms in the test group after using the compound essential oil for relieving primary dysmenorrhea;

[0032] Fig. 9 This is a graph showing the improvement of vomiting symptoms in the test group after using the compound essential oil for relieving primary dysmenorrhea;

[0033] Fig.10 This is a graph showing the improvement in headache symptoms among subjects after using a compound essential oil for relieving primary dysmenorrhea. DETAILED DESCRIPTION

[0034] The present invention is further described below in conjunction with the accompanying drawings and embodiments, but they are not intended to limit the present invention.

[0035] Source of materials: The coconut, sweet almond, black seed, green orange, cardamom, cinnamon bark, bamboo leaf prickly ash, black pepper, ginger and chastity tree used in the following examples all comply with the relevant provisions of the Chinese Pharmacopoeia (2015 edition) under the main text of each medicinal material. Before feeding, the actual medicinal materials were identified to be consistent with the names and the quality met the standards. Among them, the pulp of coconut, the kernel of sweet almond, the seed of black seed, the peel of green orange, the fruit of cardamom, bamboo leaf prickly ash, black pepper, the bark of cinnamon, the rhizome of ginger and the plant part of chastity tree were extracted.

[0036] Example 1.

[0037] The preparation method of the compound essential oil for relieving primary dysmenorrhea comprises the following steps:

[0038] 1. Wash 100 g of green mandarin peel, extract it by cold pressing at pH 7 and temperature 5°C to obtain 3-thujacene, limonene, γ-terpinene, p-cymene and myrcene;

[0039] 2. Crush 100g cardamom, 100g cinnamon bark, 100g bamboo leaf prickly ash, 100g black pepper fruit, 100g ginger rhizome, and 100g chaste tree and mix them evenly. Take 100g of the mixture and add it into a distillation apparatus, add 450mL of water, extract at 30°C, remove the heating after 300min, collect the distillation product, and obtain gingerol, α-pinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, and β-elemene;

[0040] 3. Evenly mix the extracted products obtained in steps 1 and 2 to obtain a compound essential oil for relieving primary dysmenorrhea.

[0041] Example 2.

[0042] The preparation method of the compound essential oil for relieving primary dysmenorrhea comprises the following steps:

[0043] 1. Wash 100 g of green mandarin peel, extract it by cold pressing at pH 7 and temperature 5°C to obtain 3-thujacene, limonene, γ-terpinene, p-cymene and myrcene;

[0044] 2. Crush 100g cardamom, 100g cinnamon bark, 100g bamboo leaf prickly ash, 100g black pepper fruit, 100g ginger rhizome, and 100g chaste tree and mix them evenly. Take 100g of the mixture and add it into a distillation apparatus, add 450mL of water, extract at 30°C, remove the heating after 300min, collect the distillation product, and obtain gingerol, α-pinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, and β-elemene;

[0045] 3. Wash 100 g of coconut pulp, obtain coconut oil by cold pressing, hydrolyze twice, wash with water, and then fractionate with 25 plates and reflux index of 2-9, then remove the middle fraction to obtain caprylic acid and capric acid;

[0046] 4. Wash and mix 100g sweet almond kernels and 100g black cumin seeds evenly, and extract them by cold pressing at pH 7 and temperature 5°C to obtain oleic acid, linoleic acid, palmitic acid, stearic acid, palmitoleic acid, and cis-11,14-eicosadienoic acid;

[0047] 5. Evenly mix the products extracted in steps 1, 2, 3 and 4 to obtain a compound essential oil for relieving primary dysmenorrhea.

[0048] The compound essential oil used to relieve primary dysmenorrhea was subjected to gas chromatography analysis, and the TIC spectrum obtained was as follows: Figure 1 As shown, the obtained TIC spectrum is analyzed, and the main components and contents of the compound essential oil for relieving primary dysmenorrhea are shown in Table 1.

[0049] Table 1 Contents of main components in compound essential oils used to relieve primary dysmenorrhea

[0050]

[0051]

[0052] Example 3.

[0053] The compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 1 part of limonene, 1 part of gingerol, 0.2 parts of α-pinene acetate, 0.1 parts of β-sesquiphellandrene, 0.1 parts of eucalyptol, 0.1 parts of p-cymene, 0.2 parts of β-phellandrene, 0.1 parts of γ-pinene, 0.1 parts of α-pinene, 0.1 parts of β-bisabolene, 0.1 parts of α-curcumene, 0.1 parts of anti- 0.1 part of formula-caryophyllene, 0.1 part of camphene, 0.1 part of β-pinene, 0.1 part of myrcene, 0.1 part of linalool, 0.01 part of cis-11-eicosenoic acid, 0.01 part of 3-thujacene, 0.01 part of α-farnesene, 0.01 part of sabinene, 0.01 part of 3-carene, 0.01 part of nonanoic acid, 0.01 part of α-copaene, and 0.01 part of β-elemene.

[0054] Example 4.

[0055] The compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 20 parts of limonene, 5 parts of gingerol, 2 parts of α-pinene acetate, 2 parts of β-sesquiphellandrene, 2 parts of eucalyptol, 0.5 parts of p-cymene, 0.5 parts of β-phellandrene, 1 part of γ-pinene, 1 part of α-pinene, 2 parts of β-bisabolene, 1 part of α-curcumene, 1 part of trans-caryophyllene, 1 part of camphene, 1 part of β-pinene, 0.5 parts of myrcene, 1 part of linalool, 0.1 parts of cis-11-eicosenoic acid, 0.1 parts of 3-thujacene, 0.5 parts of α-farnesene, 1 part of sabinene, 0.1 parts of 3-carene, 0.1 parts of nonanoic acid, 0.2 parts of α-copaene and 0.1 parts of β-elemene.

[0056] Example 5.

[0057] The compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 4 parts of limonene, 1 part of gingerol, 0.4 parts of α-pinene acetate, 0.4 parts of β-sesquiphellandrene, 0.3 parts of eucalyptol, 0.3 parts of p-cymene, 0.3 parts of β-phellandrene, 0.3 parts of γ-pinene, 0.2 parts of β-bisabolene, 0.2 parts of α-curcumene, 0.2 parts of anti- The invention discloses an organic solvent comprising the following: 0.2 part of hydroxyethyl-caryophyllene, 0.2 part of camphene, 0.1 part of β-pinene, 0.1 part of myrcene, 0.1 part of linalool, 0.1 part of cis-11-eicosenoic acid, 0.09 part of 3-thujacene, 0.08 part of α-farnesene, 0.08 part of sabinene, 0.07 part of 3-carene, 0.04 part of nonanoic acid, 0.04 part of α-copaene and 0.03 part of β-elemene.

[0058] Example 6.

[0059] The compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 7 parts of limonene, 3 parts of gingerol, 0.1 parts of α-pinene acetate, 0.7 parts of β-sesquiphellandrene, 0.4 parts of eucalyptol, 0.3 parts of p-cymene, 0.3 parts of β-phellandrene, 0.5 parts of γ-pinene, 0.5 parts of α-pinene, 0.8 parts of β-bisabolene, 0.5 parts of α-curcumene, 0.6 parts of trans-caryophyllene, 0.3 parts of camphene, 0.2 parts of β-pinene, 0.1 parts of myrcene. , 0.1 part of linalool, 0.07 part of cis-11-eicosenoic acid, 0.08 part of 3-thujacene, 0.2 part of α-farnesene, 0.7 part of sabinene, 0.1 part of 3-carene, 0.04 part of nonanoic acid, 0.08 part of α-copaene, 0.04 part of β-elemene, 30 parts of oleic acid, 14 parts of caprylic acid, 15 parts of capric acid, 15 parts of linoleic acid, 4 parts of palmitic acid, 2 parts of stearic acid, 0.2 part of cis-11,14-eicosadienoic acid, and 0.1 part of palmitoleic acid.

[0060] Example 7.

[0061] The compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 9 parts of limonene, 3 parts of gingerol, 0.9 parts of α-pinene acetate, 1 part of β-sesquiphellandrene, 1.3 parts of eucalyptol, 0.3 parts of p-cymene, 0.5 parts of β-phellandrene, 0.7 parts of γ-pinene, 0.6 parts of α-pinene, 0.9 parts of β-bisabolene, 0.5 parts of α-curcumene, 0.7 parts of trans-caryophyllene, 0.5 parts of camphene, 0.3 parts of β-pinene, and 0.3 parts of myrcene. 0.05 parts of cis-11-eicosenoic acid, 0.1 parts of 3-thujacene, 0.4 parts of α-farnesene, 0.7 parts of sabinene, 0.1 parts of 3-carene, 0.03 parts of nonanoic acid, 0.13 parts of α-copaene, 0.083 parts of β-elemene, 36 parts of oleic acid, 8 parts of caprylic acid, 10 parts of capric acid, 15 parts of linoleic acid, 4 parts of palmitic acid, 1 part of stearic acid, 0.3 parts of cis-11,14-eicosadienoic acid and 0.1 parts of palmitoleic acid.

[0062] Example 8.

[0063] The compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 4 parts of limonene, 1 part of gingerol, 0.4 parts of α-pinene acetate, 0.4 parts of β-sesquiphellandrene, 0.3 parts of eucalyptol, 0.3 parts of p-cymene, 0.3 parts of β-phellandrene, 0.3 parts of γ-pinene, 0.2 parts of β-bisabolene, 0.2 parts of α-curcumene, 0.2 parts of trans-caryophyllene, 0.2 parts of camphene, 0.1 parts of β-pinene and 0.1 parts of myrcene. , 0.1 parts of linalool, 0.1 parts of cis-11-eicosenoic acid, 0.09 parts of 3-thujacene, 0.08 parts of α-farnesene, 0.08 parts of sabinene, 0.07 parts of 3-carene, 0.04 parts of nonanoic acid, 0.04 parts of α-copaene, 0.03 parts of β-elemene, 30 parts of oleic acid, 21 parts of caprylic acid, 17 parts of capric acid, 14 parts of linoleic acid, 5 parts of palmitic acid, 1 part of stearic acid, 0.3 parts of cis-11,14-eicosadienoic acid, and 0.1 parts of palmitoleic acid.

[0064] Comparative example 1.

[0065] The compound essential oil for relieving primary dysmenorrhea comprises the following components in parts by weight: 3 parts of limonene, 1 part of gingerol, 1.4 parts of α-pinene acetate, 0.8 parts of β-sesquiphellandrene, 1.5 parts of eucalyptol, 0.2 parts of p-cymene, 0.3 parts of β-phellandrene, 0.5 parts of γ-pinene, 0.5 parts of α-pinene, 0.9 parts of β-bisabolene, 0.6 parts of α-curcumene, 0.7 parts of trans-caryophyllene, 0.01 parts of camphene, 0.03 parts of linalool, 0.06 parts of linalool, 0.07 parts of linalool, 0.08 parts of linalool, 0.09 parts of linalool, 0.10 parts of linalool, 0.08 parts of linalool, 0.09 ... .3 parts, β-pinene 0.2 parts, myrcene 0.2 parts, linalool 0.2 parts, 3-thujacene 1.5 parts, α-farnesene 0.2 parts, sabinene 0.7 parts, 3-carene 0.2 parts, nonanoic acid 0.03 parts, α-copaene 0.08 parts, β-elemene 0.05 parts, oleic acid 31 parts, caprylic acid 12 parts, capric acid 14 parts, linoleic acid 15 parts, palmitic acid 4 parts, stearic acid 1 part, palmitoleic acid 0.08 parts.

[0066] Effect experiment example.

[0067] 1. Population experiment:

[0068] (1) Experimental sample: compound essential oil for relieving primary dysmenorrhea (prepared in Example 2.)

[0069] (2) Experimental method: Take 4-6 drops of compound essential oil used to relieve primary dysmenorrhea, rub them in the palm of your hand to warm them up, and gently apply them on the lower abdomen from the outside to the inside, spread them evenly, and massage them repeatedly 3-4 times until they are fully absorbed.

[0070] (3) Frequency and duration of use: 1-2 times a day, start using 1-2 weeks before menstruation and stop using after menstruation.

[0071] (4)Use period: 14 days.

[0072] (5) Subjects: People who experienced lower abdominal pain, heaviness, low back pain or other discomfort before, during or after menstruation, and those who had no organic lesions. A total of 32 valid reports were obtained.

[0073] (6) The visual analogue scale (VAS) was used to evaluate the severity of dysmenorrhea before and after the use of this product, and the improvement of other accompanying symptoms before and after use was compared.

[0074] (7) Test results: The changes in VAS scores of the test subjects before and after using the compound essential oil for relieving primary dysmenorrhea provided by the present invention are shown in Table 2. It can be seen that before using this product, the proportion of people with moderate to severe dysmenorrhea (VAS score>3) reached 81.25%, and after using this product, the proportion of this group decreased to 49.99%, which shows that this product has a significant improvement effect on the pain caused by primary dysmenorrhea.

[0075] Table 2 Comparison of VAS scores of dysmenorrhea in the subjects before and after use

[0076]

[0077]

[0078] 2. Effects of using the drug on associated symptoms:

[0079] In terms of accompanying symptoms, the subjects had symptoms other than pain before use, such as Figure 2 . The accompanying symptoms mainly include fatigue, irritability, diarrhea, anxiety, nausea, dizziness, vomiting, headache, etc.

[0080] Figure 3 After the subjects used the compound essential oil for relieving primary dysmenorrhea, the improvement rate of fatigue symptoms reached 84%.

[0081] Figure 4 After the subjects used the compound essential oil for relieving primary dysmenorrhea, the improvement rate of irritability symptoms reached more than 90%.

[0082] Figure 5 After the subjects used the compound essential oil for relieving primary dysmenorrhea, the improvement rate of diarrhea was nearly 80%.

[0083] Figure 6 After the subjects used the compound essential oil for relieving primary dysmenorrhea, the improvement rate of anxiety symptoms was nearly 95%.

[0084] Figure 7After the subjects used the compound essential oil for relieving primary dysmenorrhea, the improvement rate of nausea reached 83%.

[0085] Figure 8 After the subjects used the compound essential oil for relieving primary dysmenorrhea, the improvement rate of dizziness reached 75%.

[0086] Fig. 9 The improvement of vomiting symptoms after the test group used the compound essential oil to relieve primary dysmenorrhea, wherein the improvement rate of vomiting after using Example 2 reached 50%.

[0087] Fig.10 After the subjects used the compound essential oil for relieving primary dysmenorrhea, the improvement rate of headache symptoms reached 100%.

[0088] From the above results, it can be seen that among the test population, a large number of people have symptoms such as fatigue, irritability, diarrhea, anxiety, etc. After using the compound essential oil provided in this test for relieving primary dysmenorrhea, the above symptoms have been effectively improved, and other symptoms such as nausea, dizziness, headache, etc. have also been alleviated to a certain extent. At the same time, no obvious side effects were shown in the test population.

[0089] The above description is only an example of the implementation mode of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A compound essential oil for relieving primary dysmenorrhea, characterized in that: The invention comprises the following components in parts by weight: 1-20 parts of limonene, 1-5 parts of gingerol, 0.1-2 parts of α-pinene acetate, 0.1-2 parts of β-sesquiphellandrene, 0.1-2 parts of eucalyptol, 0.1-0.5 parts of p-cymene, 0.1-0.5 parts of β-phellandrene, 0.1-1 parts of γ-pinene, 0.1-1 parts of α-pinene, 0.1-2 parts of β-bisabolene, 0.1-1 parts of α-curcumene, 0.1-1 parts of trans-caryophyllene, 0.1-1 parts of camphene. .1-1 part, β-pinene 0.1-1 part, myrcene 0.1-0.5 part, linalool 0.1-1 part, cis-11-eicosenoic acid 0.01-0.1 part, 3-thujacene 0.01-0.1 part, α-farnesene 0.01-0.5 part, sabinene 0.01-1 part, 3-carene 0.01-0.1 part, nonanoic acid 0.01-0.1 part, α-copaene 0.01-0.2 part, β-elemene 0.01-0.1 part; It also includes vegetable oil, which includes the following components in parts by weight: 25-45 parts of oleic acid, 1-30 parts of caprylic acid, 5-25 parts of capric acid, 5-20 parts of linoleic acid, 1-10 parts of palmitic acid, 1-5 parts of stearic acid, 0.1-0.5 parts of cis-11,14-eicosadienoic acid, and 0.1-1 parts of palmitoleic acid.

2. The compound essential oil for relieving primary dysmenorrhea according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 2-10 parts of limonene, 1-3 parts of gingerol, 0.1-1 parts of α-terpinene acetate, 0.2-1 parts of β-sesquiphellandrene, 0.2-1.5 parts of eucalyptol, 0.2-0.4 parts of p-cymene, 0.3-0.5 parts of β-phellandrene, 0.2-0.8 parts of γ-terpinene, 0.1-0.7 parts of α-pinene, 0.1-1 parts of β-bisabolene, 0.1-0.5 parts of α-curcumene, 0.2-0.8 parts of trans-caryophyllene, 0.1-0.5 parts of camphene, 0.1-0.5 parts of β-pinene, 0.1-0.5 parts of myrcene, and 0.1-0.5 parts of linalool. , 0.05-0.1 parts of cis-11-eicosenoic acid, 0.05-0.1 parts of 3-thujacene, 0.05-0.1 parts of α-farnesene, 0.05-1 parts of sabinene, 0.05-0.1 parts of 3-carene, 0.01-0.05 parts of nonanoic acid, 0.03-0.15 parts of α-copaene, 0.02-0.08 parts of β-elemene, 30-40 parts of oleic acid, 5-25 parts of caprylic acid, 10-20 parts of capric acid, 10-15 parts of linoleic acid, 3-5 parts of palmitic acid, 1-3 parts of stearic acid, 0.1-0.3 parts of cis-11,14-eicosadienoic acid, and 0.1-0.5 parts of palmitoleic acid.

3. The compound essential oil for alleviating primary dysmenorrhea according to claim 2, characterized in that: The invention comprises the following components in parts by weight: 4 parts of limonene, 1 part of gingerol, 0.4 parts of α-pinene acetate, 0.4 parts of β-sesquiphellandrene, 0.3 parts of eucalyptol, 0.3 parts of p-cymene, 0.3 parts of β-phellandrene, 0.3 parts of γ-pinene, 0.2 parts of β-bisabolene, 0.2 parts of α-curcumene, 0.2 parts of trans-caryophyllene, 0.2 parts of camphene, 0.1 parts of β-pinene, 0.1 parts of myrcene, 0.1 parts of linalool. 0.04 parts of alpha-copaene, 0.03 parts of beta-elemene, 30 parts of oleic acid, 21 parts of caprylic acid, 17 parts of capric acid, 14 parts of linoleic acid, 5 parts of palmitic acid, 1 part of stearic acid, 0.3 parts of cis-11,14-eicosadienoic acid and 0.1 parts of palmitoleic acid.

4. The method for preparing the compound essential oil for alleviating primary dysmenorrhea according to any one of claims 1 to 3, characterized in that: The following steps are included: A. Wash 1-2 parts of green mandarin peel by weight, extract it by cold pressing method, and obtain 3-thujene, limonene, γ-terpinene, p-cymene, and myrcene; B. By weight, 1-2 parts of cardamom, 1-2 parts of bamboo-leaf prickly ash, 1-2 parts of black pepper fruit, 1-2 parts of cinnamon bark, 1-2 parts of ginger rhizomes, and 1-2 parts of chaste tree plants are crushed and mixed evenly, and extracted by steam distillation to obtain gingerol, α-pinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, and β-elemene; C. Wash 1-2 parts of coconut pulp by weight, extract coconut oil by cold pressing, and extract by fractionation after hydrolysis to obtain caprylic acid and capric acid; D. Wash 1-2 parts of sweet almond kernels and 1-2 parts of black cumin seeds by weight, extract them by cold pressing method, and obtain oleic acid, linoleic acid, palmitic acid, stearic acid, palmitoleic acid, and cis-11,14-eicosadienoic acid; E. Mix 3-thujacene, limonene, γ-terpinene, p-cymene, myrcene, gingerol, α-terpinene acetate, β-sesquiphellandrene, eucalyptol, β-phellandrene, α-pinene, β-bisabolene, α-curcumene, trans-caryophyllene, camphene, β-pinene, linalool, cis-11-eicosenoic acid, α-farnesene, sabinene, 3-carene, nonanoic acid, α-copaene, β-elemene, caprylic acid, capric acid, oleic acid, linoleic acid, palmitic acid, stearic acid, palmitoleic acid, and cis-11,14-eicosadienoic acid in parts by weight.

5. The method for preparing the compound essential oil for alleviating primary dysmenorrhea according to claim 4, characterized in that: In step A, the pH value of the cold pressing extraction is 6-8 and the temperature is 3-7°C; in step B, the temperature of the steam distillation extraction is 28-32°C and the solid-liquid ratio is (4-6):1; in step C, the number of distillation plates is 25 and the reflux index is 2-9; in step D, the pH value of the cold pressing extraction is 6-8 and the temperature is 3-7°C.

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