Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method

A technology of Enterococcus faecium and culture medium, which is applied in the field of Enterococcus faecium and its high-density solid-state fermentation medium, can solve the problems of easy feedback control, easy inactivation of strains, high cost, etc., and achieve good economic benefits and live bacteria The effect of high concentration and low production cost

Active Publication Date: 2015-12-09
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, most of the products are in the form of liquid fermentation, but the cost is high, they are easily subject to feedback control, and it is difficult to increase the amount of viable bacteria
Moreover, after the liquid product is fermented, the strain is easily inactivated, resulting in a short shelf life of the product and affecting the marketing of the product
Another common form of fermentation is solid-state fermentation, and the cost of solid-state fermentation medium is relatively high at this stage. In some solid-state fermentation processes, bacteria cannot tolerate drying and low-moisture storage in the later stage, so it is difficult to increase the amount of bacteria

Method used

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  • Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method
  • Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method
  • Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Isolation, screening, identification and preservation of Enterococcus faecium

[0041] 1.1 Isolation of strains

[0042] Take dry cheese samples, grind them, and dilute them 10, 100, and 1000 times with sterile water, respectively, take 200ul and spread them on MRS solid medium, culture them at 37°C for 48 hours, pick colonies, streak MRS medium, and After 4 times of purification, a single colony was picked and cultured in MRS broth, 20% glycerol, and stored at -70°C for future use. After Gram staining, catalase test, nitrate reduction test and 16SrDNA sequencing.

[0043] 1.2 Identification of strain morphological characteristics

[0044] Take a single colony of the purified strain (GLM105), transfer it to MRS solid medium (agar), and culture it in a constant temperature incubator at 37°C for 24h, 36h, and 48h, and observe the size, color, edge, protrusion, and Smoothness, viscosity, transparency and other characteristics. The results showed that strain G...

Embodiment 2

[0087] Embodiment 2 A kind of culture medium of high-density solid-state fermentation Enterococcus faecium

[0088] The formula of the solid-state fermentation medium is: 22% wheat bran, 22% soybean meal, 4% sugar cane molasses, 3% activated carbon, 5% calcium carbonate, 4% β-cyclodextrin, and 40% water content, and the percentages are all by mass . (except that the formula is, the formula of the solid-state fermentation medium can be: wheat bran 15%~25%, soybean meal 19%~29%, sugarcane molasses 3%~5%, activated carbon 2%~4%, calcium carbonate 2% ~6%, β-cyclodextrin 2% ~ 6%, water content 40% ~ 50%, the percentages are mass percentages.)

[0089] The preparation method of the above-mentioned medium: first configure the basic medium (wheat bran, soybean meal and water) according to the conventional method, then add sugarcane molasses, activated carbon, calcium carbonate, β-cyclodextrin, stir well, and autoclave at 115°C for 15 minutes , and cool down. Wheat bran and soybean ...

Embodiment 3

[0090] Embodiment 3 A kind of method of high-density solid-state fermentation culture Enterococcus faecium

[0091] 1) Preparation of strains and their seed solution

[0092] The Enterococcus faecium GLM105 strain was activated on the MRS agar plate, and a single colony was picked and inoculated into the MRS liquid test tube, cultured at 37°C for 24 hours, then transferred to the MRS liquid medium with 2% v / v inoculum, and cultured for 24 hours as the seed solution.

[0093] The main components of MRS medium are peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, Tween 801.0mL, sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, sulfuric acid Magnesium 0.58g, manganese sulfate 0.25g, distilled water 1000mL, pH6.2~6.6. The optimum pH is 6.0, the optimum temperature is 37°C, facultative anaerobic.

[0094] 2) Vaccination

[0095] With 3% v / v inoculation amount, the prepared seed liquid is inoculated into the substratum...

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Abstract

The invention discloses enterococcus faecium, a culture medium thereof for high-density solid-state fermentation, and a high-density solid-state fermentation method, wherein the preservation number of the bacterial strain is CGMCC No.11155. Enterococcus faecium has the excellent performances of inhibiting bacteria, resisting acid and cholate, and the like, is tolerant to low moisture, and has relatively long shelf life under solid state fermentation condition. The culture medium contains wheat bran, bean pulp, cane molasses, activated carbon, calcium carbonate and beta- cyclodextrine. In the preparation technology, lactobacillus is inoculated into the culture medium for fermentation, and then drying is carried out at 45-65 DEG C, and thus obtaining a fermentation product. Compared with the like enterococcus faecium strains, the strain and the fermentation product provided by the invention have the advantages of high concentration of viable organisms, long shelf life and the like. The solid-state fermentation product of the strain provided by the invention can significantly improve the daily gain, the survival rate and the feed conversion ratio of nursery pigs, is obvious in economic benefit, and can be taken as a feed additive.

Description

technical field [0001] The invention relates to a culture medium and method for Enterococcus faecium and its high-density solid-state fermentation. Background technique [0002] Micro-ecological feed additives are live bacterial preparations that are safe and beneficial to humans and animals and are made from known beneficial microorganisms through special processes such as cultivation, fermentation, and drying, and are used as feed additives. They are generally referred to as micro-ecological preparations and are currently on the market. There are two types of probiotics, liquid and solid. Lactic acid bacteria, as an important type of microecological preparations, can regulate intestinal dysfunction and maintain the balance of intestinal bacteria; when used in feed, they can promote growth, prevent diseases and improve the quality of poultry products. [0003] At present, there are many products of lactic acid bacteria probiotics. Among them, the products in the form of l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A01P1/00C12R1/46
Inventor 张宏刚李莉
Owner GUANGZHOU GLAM BIOTECH
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