Compound biological agent for enabling acidified marsh gas fermentation system to restore gas production and preparation method thereof

A technology for biological preparations and fermentation systems, applied in biochemical equipment and methods, microorganism-based methods, fermentation, etc., and can solve problems such as long-term domestication, large amounts of manpower, material resources, and losses

Active Publication Date: 2014-03-26
深圳克格瑞环保生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, restarting not only requires a lot of manpower and material resources, but also requires a long time of domestica...

Method used

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  • Compound biological agent for enabling acidified marsh gas fermentation system to restore gas production and preparation method thereof
  • Compound biological agent for enabling acidified marsh gas fermentation system to restore gas production and preparation method thereof
  • Compound biological agent for enabling acidified marsh gas fermentation system to restore gas production and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The preparation of the mixed bacterial liquid of embodiment 1 acidophilic acid-resistant methanogen

[0063] (1) Preparation of Methanobacterium espanolae single bacterial solution

[0064] According to the medium formula numbered DSM No.506 provided by the German Culture Collection Center, the volume percentage content is 80% H 2 +20%CO 2 Prepare the liquid medium of Methanobacterium espanolae under the anaerobic conditions, adjust the pH value to 5.6-6.2, and pack them in 25mL anaerobic tubes, 500mL anaerobic bottles and 20L anaerobic tanks under the above anaerobic conditions, and the filling volume is It is advisable not to exceed 20% of the total volume of the anaerobic tube, anaerobic bottle or anaerobic tank. Cover the airtight cover and sterilize at 121°C for 20 minutes. Filled with 80% H by volume 2 +20%CO 2 The gas is inflated to an anaerobic tube or anaerobic bottle or anaerobic tank with a gas pressure of 100kPa. The live bacterial culture of Methanobac...

Embodiment 2

[0075] Example 2 Preparation of mixed bacterial liquid of acidophilic and acid-resistant methanogens

[0076] According to the needs of production, the scale of cultivation can be expanded and four-level cultivation can be carried out. Set 1m 3 The anaerobic tank is used as four-level culture, and the medium formula, inoculation method, culture conditions, and mixed volume ratio of the mixed bacterial solution of the corresponding bacterial species are the same as in Example 1.

Embodiment 3

[0077] The preparation of the mixed bacteria liquid of embodiment 3 intertrophic acetic acid oxidizing bacteria

[0078] (1) Tepidanaerobacter acetatoxydans single bacterial solution preparation

[0079] According to the medium formula numbered DSM No.1301 provided by the German Culture Collection Center, the volume percentage content is 80% N 2 +20%CO 2 Prepare the liquid culture medium of Tepidanaerobacter acetatoxydans under the anaerobic conditions, adjust the pH value to 7.0-7.2, and pack them in 25mL anaerobic tubes, 500mL anaerobic bottles and 20L anaerobic tanks under the above-mentioned anaerobic conditions. It is advisable not to exceed 50% of the total volume of the anaerobic tube, anaerobic bottle or anaerobic tank. Cover the airtight cover and sterilize at 121°C for 20 minutes. Filled with a volume percentage of 80% N 2 +20%CO 2 The gas is inflated to an anaerobic tube or anaerobic bottle or anaerobic tank with a gas pressure of 100kPa. The live bacterial cul...

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Abstract

The invention relates to a compound biological agent for enabling an acidified marsh gas fermentation system to restore gas production and a preparation method thereof. The compound biological agent contains acidophilus acid-resistant methane-producing bacteria, syntrophic acetic acid oxidation bacteria and minor elements. The compound biological agent enables an acidified marsh gas fermentation system to quickly restore normal gas production without adding alkaline substances or performing massive material discharging or changing, thus ensuring continuous stable gas production of the marsh gas fermentation system. The invention also provides a preparation method of the compound biological agent, which comprises the following steps: under anaerobic conditions, mixing a mixed bacterium solution of acidophilus acid-resistant methane-producing bacteria and a mixed bacterium solution of syntrophic acetic acid oxidation bacteria to obtain a compound bacterium solution, concentrating until the total concentration of cells of the acidophilus acid-resistant methane-producing bacteria and the syntrophic acetic acid oxidation bacteria is at least 1*10<9>/mL, and then adding the minor elements; and packaging, preserving at low temperature, and preparing into the liquid compound biological agent for enabling an acidified marsh gas fermentation system to restore gas production. A solid agent can be further prepared by stirring and mixing with auxiliary materials and then performing anaerobic drying.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and relates to a compound biological preparation for recovering gas production in an acidification biogas fermentation system and a preparation method thereof. The compound biological preparation is mainly used for various types of biogas fermentation in which gas production is reduced or stopped due to acidification The system can restore the normal gas production of the biogas fermentation system. Background technique [0002] The biogas fermentation system has been widely used to process various organic wastes, and obtain relatively clean biogas energy, and finally obtain organic fertilizers such as biogas residue and biogas slurry. Raw materials for biogas fermentation include crop straw, livestock and poultry farming waste, light industrial waste water and residue, domestic organic waste, sewage sludge, etc. According to the statistics of relevant departments, a total of 45 ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P39/00C12P5/02C02F11/04C12R1/145C12R1/01
CPCY02E50/30
Inventor 李东刘晓风闫志英袁月祥廖银章李志东
Owner 深圳克格瑞环保生物科技有限公司
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