Highly efficient and low-cost microbiological feed proteins based on vinegar residue and miscellaneous meal

Abstract

The invention relates to the field of producing highly efficient and low-cost microbiological feed proteins based on the three-stage fermentation of vinegar residue and miscellaneous meal by using probiotics. The vinegar residue used as a main carbon source and the cottonseed meal used as a main nitrogen source as well as a partial carbon source are subjected to the first-stage fermentation pretreatment by using food-grade mould, the product is subjected to the second-stage fermentation by using bacillus and yeast as zymocytes and adding partial cottonseeds, and finally the product is added with partial soybean meal and rapeseed meal used as a nitrogen source as well as a partial carbon source, inoculated with lactobacillus and acid-resisting bifidobacterium and uniformly mixed, and then the product is subpackaged in one-way membrane bio-bags for sealed storage, namely the finished products are obtained by the three-stage fermentation. The microbiological feed proteins are extremely low in production cost and can be used as a feed component, and the adding amount reaches 5 to 40 percent. Because the finished products do not need drying treatment, the finished products have more viable bacteria as well as enzymes, few other bacteria and an obvious feeding effect; moreover, the microbiological feed proteins also have the advantages of greatly improving the utilization rate of livestock and poultry feed, increasing yield, improving intestinal environment, improving immunity and resistance, replacing antibiotics and improving quality of animal products.

Description

technical field [0001] The invention belongs to the field of microbial feed additives, in particular to novel microbial feed protein or microbial feed additives based on fibrous brewing waste with high efficiency and low cost. Background technique [0002] At present, the profit of the feed industry is very low, and most of them use grain as the raw material, so the cost is difficult to reduce. The microbial feed that can fully improve the utilization rate of feed and reduce the cost of feed brings new hope to the feed industry and aquaculture industry. As we all know, there are a large number of microorganisms in the gastrointestinal tract of animals. These microorganisms, as well as between microorganisms and animals, form an inseparable whole that is interdependent and interacting—the microecosystem. Probiotics are the main live bacteria component of this micro-ecosystem, and are of great significance to the growth, development, digestion, absorption, nutrition and immuni...

AI Technical Summary

Problems solved by technology

The second is that bio-fermented feed based on grain or common industrial fermentation raw materials also needs low-temperature drying or freeze-drying storage treatment. Generally speaking, the cost is high, the amount of feed is small, and the biological activity is not obvious! Such as invention patents (CN1682599A), (CN1806659A), (CN101190003A), (CN102204632A)

The third is based on straw, distiller's grains and other wastes, treated with Trichoderma or cellulase, or fermented with probiotics, and dried at low temperature for storage. The raw materials also need to be sterilized, which is costly, but the active ingredients are not easy to preserve, and the effect of probiotics is not obvious. , such as invention patents (CN101897381B), (CN100337555C)

[0008] In previous studies, such as invention patents (CN1285156A), (CN101491290A), (CN101366445A), fresh distiller's grains were partially dried, which consumed a lot of energy, and was inoculated with a Geotrichum candidum or Paecilomyces for degradation, but the lignin effect Not obvious, the product has no probiotic effect

Another example is the invention patent (CN101919490A) (CN101756009A) and (CN101756044A), the vinegar grains are not pretreated by flora rich in ligninase and cellulase, and only by adding cellulase, etc., the cost is high and the effect is not obvious. Moreover, aerobic probiotics and anaerobic probiotics are not fermented in stages, resulting in poor fermentation on both sides

Invention patent (CN101756012A) has a second-stage fermentation, but the first-stage Aspergillus niger enzyme does not degrade lignin and cellulose significantly, and the second-stage is also aerobic fermentation without probiotic fermentation

The products of the above three inventions must be dried at low temperature, which consumes a lot of energy and has a low preservation rate of bacterial enzymes.

Therefore, the production cost is high, and the feeding effect is not obvious, which is not conducive to the promotion of industrialization