37 results about "Cell culture study" patented technology

The invention discloses an in vitro cell culture device. The in vitro cell culture device comprises a bioreactor, a double nutrient solution irrigating system, a loading device and a monitoring system. The invention also discloses a method for culturing the cell by using the in vitro cell culture device. The method comprises the following steps: biologically simulating a physiological status, establishing the double nutrient solution irrigating system, and supplying different nutrients to different tissue blocks; allocating two pipe systems for each double culture cup, taking the culture tissue as a spacer layer of two culture solutions, culturing by simultaneously using two culture solutions, automatically irrigating the culture solutions, timely and quantificationally changing the cell culture solution, regulating the irrigating speed of the culture solution and constantly providing various nutrients and stable pH value for the cell. A dynamic pressurized environment is adopted for the cell culture device and the cell culture method; a pressure is exerted on the culture tissue by exerting a controllable axial compressive deformation load; the pressure and the compressive deformation of each culture tissue can be monitored and analyzed in real time and the pressure can be adjusted.

The invention relates to a multifunctional bioreactor used for cell culture and cell sorting. The multifunctional bioreactor comprises a turnover device and a cell culture vessel, wherein the turnover device contains a turnover support which can operate around a space axis and a magnetic field generating device in physical connection with the turnover device, the cell culture vessel is detachably fixed on the turnover support, the cell culture vessel is located on the space axis or an extension line thereof and operates along with the turnover support according to certain rules and tracks, the end face of the cell culture vessel is close to the magnetic field generating device, and the magnetic field generating device can control change of a magnetic field surrounding the periphery and interior of the cell culture vessel. The multifunctional bioreactor provided by the invention has the advantages that co-culture on adherent cells and suspension cells is carried out without replacing the vessel, stirring inside a culture solution is realized by virtue of movement of carriers in the vessel in a magnetic field environment, cell contamination, target cell damage and death risk are reduced, and the vessel can be taken as a bearing material of the adherent cells, so that cell culture efficiency is greatly improved.

The invention discloses a cell culture device. The cell culture device comprises a control system, a cold closet, a liquid adding mechanism, a first centrifugal machine, a second centrifugal machine, a plasma preparation device, an incubator, a video viewing system and a laminar cleaning system which are integrated in a machine body. The plasma preparation device is integrated in the cell culturedevice and is used for activating plasma to obtain activated plasma; when the cell culture is performed, the activated plasma is added during the induction period and the growth culture period; and because the activated plasma is homologous with cultured cells, the cultured cells are easier to accept culture environment, so that the yield of the cultured cells is improved. In addition, various components of the cell culture device are all integrated together, no special spatial environment and place is needed, the whole process is entirely and automatically executed by the device, and the contact of human bodies is avoided, so that the chance of operation pollution is reduced, the cleanliness during the cell culture is furthest guaranteed, and the quality of the cultured cells can be improved.

The invention discloses a preparation method of bionic injectable gelatin-silane composite medical hydrogel. The preparation method comprises the following steps: dissolving food and pharmaceutical grade gelatin powder in a phosphate buffer solution to obtain a gelatin solution; dissolving silane in a neutral phosphate buffer solution to obtain a silane solution; adding the silane solution into the gelatin solution according to a ratio of silane to gelatin of 0.5-2 ml to 1 g for carrying out a hydrolysis reaction to obtain transparent gelatin-silane composite sol; and gelatinizing the gelatin-silane composite sol at 37 DEG C. The bionic injectable gelatin-silane composite medical hydrogel disclosed by the invention contains no organic solvent or toxic component, and the formed composite medical hydrogel has high mechanical flexibility, maintains high stability in the phosphate buffer solution and has strong swelling resistance and approximately linear biodegradability; cell culture studies have shown that the bionic composite hydrogel prepared by the preparation method disclosed by the invention has good cell compatibility, thereby being capable of being applied to a variety of fields of soft tissue and bone tissue repair, tissue engineering and regeneration.

A versatile compartmentalized cell culture device, with a selectively permeable membrane separating the compartments, provides many attributes relative to traditional devices. It can be configured for high-density cell culture, co-culture, and sample dialysis while rolling or standing still. It can also be configured for continuous movement of liquid between compartments. The wide combination of attributes not found in other membrane based cell culture and bioprocessing devices includes more cell capacity, more cell secreted product capacity, higher cell and product density, increased medium capacity, minimized use of exogenous growth factors, compatibility with standard cell culture equipment and protocols, increased scale up efficiency, capacity to function when rolling or standing still, capacity for perfusion without the need for pumps, and more efficient sample dialysis.

A method for stem or progenitor cell culture. More precisely, the invention relates to a method for cell culture using one or more IαI (inter-alpha trypsin inhibitor or Inter-alpha inhibitor) protein(s) or part(s) thereof as a component in a cell culture media or a coating on a cell culture surface material. Furthermore the invention relates to a cell culture media and a cell culture coating/matrix provided with one or more IαI proteins(s) or part(s) thereof.

The invention discloses a culture method of immune cells, comprising the following steps: (1) collecting peripheral blood mononuclear cells; (2) inoculating the cells into a culture flask containing aculture medium for culture; (3) introducing the cells cultured in the step (2) into a culture bag, wherein the volume of the culture liquid is smaller than the volume of the culture bag; (4) Harvestimmune cells. The invention provides a culture method of immune cells, by controlling the proportion of the culture liquid volume and the volume of the culture bag in the culture process, the expansion ability of cells and the number of cells finally obtained are improved, the proliferation of immune cells is realized in large quantities, the utilization rate of the culture solution is improved, and the cost is saved.

Disclosed are microbeads for cell culture and a method of monitoring cell culture using the same. More particularly, each of the microbeads for cell culture according to an embodiment of the present invention include a core and a surface modification layer formed on a surface of the core. By using the method of monitoring cell culture with the microbeads for cell culture according to an embodiment of the present invention, cell culture may be carried out in highly scaled-up dimension and easily monitored.

The invention discloses an immobilized cell culture method, relating to the technical field of cell culture, for increasing media obtained by superficial cells in cellular layers within unit time, so as to improve the yield of immobilized culture of the cells. The immobilized cell culture method comprises the following steps: inoculating the cells onto a support layer, so as to form a cell layer attached to the support layer; and supplying media to the support layer, wherein the media wet the support layer and the cell layer, a plurality of first channels are formed in the cell layer, and the media are stored in the first channels. The immobilized cell culture method provided by the invention is used for culturing of algae, fungi and bacteria.

The invention relates to the field of biological medicine, in particular to a cell culture method and medium for preparing human interferon betala and application of the cell culture medium. According to the technical scheme of the invention, the cell culture medium for preparing the human interferon betala is provided, and the ingredients of the cell culture medium consist of a basal culture medium and additives, wherein the concentration of the basal culture medium is 12-18 g/L, and the additives contain the following ingredients of: D-galactose with the concentration of 0.05-0.45 g/L, D-mannitol with the concentration of 0.3-0.7 g/L, N-acetyl glucosamine with the concentration of 0.10-0.50 g/L, and D-glucose with the concentration of 1.0-5.0 g/L. According to the serum-free culture medium and method, the problems of low yield and low activity in the cell culture of the recombinant human interferon betala, are solved, the biological activity of the interferon betala in the cell collection liquid finally obtained by the cell culture method reaches 1*10<6> IU/ml, and the content of the interferon betala reaches over 0.8 g/L through the detection of an ELISA method.

The invention relates to the technical field of cell culture, in particular to an isolated culture method for cartilage cells. The isolated culture method comprises the following steps: treating a cartilage tissue into tissue blocks, adding trypsin for digestion, and then adding type II collagenase for digestion, wherein the concentration by mass percentage of the type II collagenase is 0.05 to 0.1 percent, and the digestion time of the type II collagenase is 16 to 20 hours. An experimental research shows that by the adoption of proper concentration and digestion time of type II collagenase for digestion of the cartilage tissue, the vitality of the cartilage cells can be effectively retained, so that enough number of cartilage cells can be obtained within relatively short time during later multiplication culture of the cartilage cells obtained by separation, and the cell culture efficiency is greatly improved.

The present invention relates to systems and methods for optimising the usage of laboratory and cell culturing space for cell culture and biomanufacturing. The invention provides a system and method that can be used to provide a plurality of workstations and/or storage bays for bioreactors in cell culturing and/or biomanufacturing facilities.

The invention relates to a dual-media approach for culturing isolated corneal endothelial cells. Isolated corneal endothelial cells are first contacted with a proliferative medium to propagate and/or expand the endothelial cells followed by a maintenance medium to preserve the morphology and/or characteristics of the corneal endothelial cells. The invention includes the proliferative medium and the maintenance medium and also a combination of the two medium.

The invention provides a skeletal muscle cell in-vitro efficient culture method, and belongs to the field of cell culture. The method includes the steps of preparing a test tool, extracting a skeletal muscle, digesting the skeletal muscle, preparing skeletal muscle suspension, and culturing the skeletal muscle suspension. The method is direct and effective when fibroblasts are removed through a differential iron wall method, the mixing of surface enveloped cells and bacteria is ingeniously avoided, cell and bacteria contamination is reduced, and meanwhile the purity of target cells is greatly improved and can approach 100% during cell separation. By means of the method, high-purity, high-survival-rate and high-activity skeletal muscle cells extremely low in contamination risk can be obtained, the cultured cells are good in differentiation and high in cell activity, and the method has great popularization significance.

An integrated flow source is a limiting factor in numerous microfluidic applications. In addition to precise gradients and controlling molecular transports, a built-in source of stable and accurate flow can enable novel shear stress modulations for long-term cell culturing studies. The Tesla turbine, when used as a pump on the microfluidic regime, produces stable and accurate fluid gradients by utilizing laminar flow between its rotating discs Utilizing a stereolithography based 3D printer, a tesla pump (Ø10 cm) and associated housing capable of driving a microfluidic gradient is provided having a printed rotor surface topology of the pump in order to enhance pumping of biological fluids like blood at elevated viscosities. The surface topology is tuned via 3D pixilation, and this modulation completely recovered the pressure loss between pumping water at 1 cP versus glycerol solution at 3 cP. As a result, increased fluid viscosities, and even Non-Newtonian viscosities, can be used.