The invention discloses a cell culture device. The cell culture device comprises a control system, a cold closet, a liquid adding mechanism, a first centrifugal machine, a second centrifugal machine, a plasma preparation device, an incubator, a video viewing system and a laminar cleaning system which are integrated in a machine body. The plasma preparation device is integrated in the cell culturedevice and is used for activating plasma to obtain activated plasma; when the cell culture is performed, the activated plasma is added during the induction period and the growth culture period; and because the activated plasma is homologous with cultured cells, the cultured cells are easier to accept culture environment, so that the yield of the cultured cells is improved. In addition, various components of the cell culture device are all integrated together, no special spatial environment and place is needed, the whole process is entirely and automatically executed by the device, and the contact of human bodies is avoided, so that the chance of operation pollution is reduced, the cleanliness during the cell culture is furthest guaranteed, and the quality of the cultured cells can be improved.