2 results about "Cell culture study" patented technology

This invention discloses a temperature gradient cell capture culture chip device and method, comprising a cell capture culture chip, a temperature conduction film, and a temperature gradient chip. The cell capture culture chip is provided with an inlet, an inlet channel, an outlet channel, and an outlet, with the inlet and outlet channels correspondingly connected to cell capture culture chambers. The temperature gradient chip is provided with several heating channels, the interior of which is filled with liquid metal material. Each heating channel includes several semi-annular cavities and several linear cavities. The semi-annular cavities on the heating channels combine to form a circular heating area. This invention enables on-chip cell culture studies under dual-factor stimulation conditions of drug concentration gradient and temperature gradient, supporting efficient localization and long-term stable culture of target cells. It can also synergistically apply dual-factor stimulation conditions of drug concentration gradient and temperature gradient, allowing real-time observation of cell proliferation behavior under these stimulation conditions and subsequent metabolite and genomic analysis.

This invention discloses a cell culture chip device and method for multiple factors including concentration, temperature, and light intensity. The device includes a light gradient chip, a cell capture culture chip, a temperature conduction film, and a temperature gradient chip. The temperature gradient chip has several heating channels filled with liquid metal material. The light gradient chip has an inlet, an inlet channel, a light gradient unit, an outlet channel, and an outlet. An infusion channel connects the inlet and outlet channels, and the infusion channel has a light-absorbing reservoir chamber. This invention enables on-chip cell culture studies under three-factor stimulation conditions: drug concentration gradient, temperature gradient, and light intensity gradient. It supports efficient localization and long-term stable culture of target cells and can synergistically apply these three-factor stimulation conditions. It allows real-time observation of cell proliferation behavior under these stimulation conditions and subsequent metabolite and genomic analysis.