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38 results about "Cell culture study" patented technology

Cell culture system

ActiveUS20140038279A1Bioreactor/fermenter combinationsFungi3D cell cultureMass cell culture
The embodiments of the invention described herein relate to systems and methods for culturing and / or maintaining intestinal cells, tissues and / or organoids in vitro. The cells, tissues and / or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.
Owner:PRESIDENT & FELLOWS OF HARVARD COLLEGE

In vitro cell culture device and culture method

InactiveCN104762206AControl perfusion rateRegular and quantitative replacementBioreactor/fermenter combinationsBiological substance pretreatmentsCulture cellCulture fluid
The invention discloses an in vitro cell culture device. The in vitro cell culture device comprises a bioreactor, a double nutrient solution irrigating system, a loading device and a monitoring system. The invention also discloses a method for culturing the cell by using the in vitro cell culture device. The method comprises the following steps: biologically simulating a physiological status, establishing the double nutrient solution irrigating system, and supplying different nutrients to different tissue blocks; allocating two pipe systems for each double culture cup, taking the culture tissue as a spacer layer of two culture solutions, culturing by simultaneously using two culture solutions, automatically irrigating the culture solutions, timely and quantificationally changing the cell culture solution, regulating the irrigating speed of the culture solution and constantly providing various nutrients and stable pH value for the cell. A dynamic pressurized environment is adopted for the cell culture device and the cell culture method; a pressure is exerted on the culture tissue by exerting a controllable axial compressive deformation load; the pressure and the compressive deformation of each culture tissue can be monitored and analyzed in real time and the pressure can be adjusted.
Owner:THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV

Multifunctional bioreactor used for cell culture and cell sorting

The invention relates to a multifunctional bioreactor used for cell culture and cell sorting. The multifunctional bioreactor comprises a turnover device and a cell culture vessel, wherein the turnover device contains a turnover support which can operate around a space axis and a magnetic field generating device in physical connection with the turnover device, the cell culture vessel is detachably fixed on the turnover support, the cell culture vessel is located on the space axis or an extension line thereof and operates along with the turnover support according to certain rules and tracks, the end face of the cell culture vessel is close to the magnetic field generating device, and the magnetic field generating device can control change of a magnetic field surrounding the periphery and interior of the cell culture vessel. The multifunctional bioreactor provided by the invention has the advantages that co-culture on adherent cells and suspension cells is carried out without replacing the vessel, stirring inside a culture solution is realized by virtue of movement of carriers in the vessel in a magnetic field environment, cell contamination, target cell damage and death risk are reduced, and the vessel can be taken as a bearing material of the adherent cells, so that cell culture efficiency is greatly improved.
Owner:NANJING XINNUODAN BIOTECH

Preparation method of bionic injectable gelatin-silane composite medical hydrogel

The invention discloses a preparation method of bionic injectable gelatin-silane composite medical hydrogel. The preparation method comprises the following steps: dissolving food and pharmaceutical grade gelatin powder in a phosphate buffer solution to obtain a gelatin solution; dissolving silane in a neutral phosphate buffer solution to obtain a silane solution; adding the silane solution into the gelatin solution according to a ratio of silane to gelatin of 0.5-2 ml to 1 g for carrying out a hydrolysis reaction to obtain transparent gelatin-silane composite sol; and gelatinizing the gelatin-silane composite sol at 37 DEG C. The bionic injectable gelatin-silane composite medical hydrogel disclosed by the invention contains no organic solvent or toxic component, and the formed composite medical hydrogel has high mechanical flexibility, maintains high stability in the phosphate buffer solution and has strong swelling resistance and approximately linear biodegradability; cell culture studies have shown that the bionic composite hydrogel prepared by the preparation method disclosed by the invention has good cell compatibility, thereby being capable of being applied to a variety of fields of soft tissue and bone tissue repair, tissue engineering and regeneration.
Owner:XI AN JIAOTONG UNIV

Compartmentalized device for cell culture, cell processing and sample dialysis

ActiveUS20110212519A1More efficient dialysis of laboratory samplesImprove concentrationBiochemistry apparatusVertebrate cellsHigh cell3D cell culture
A versatile compartmentalized cell culture device, with a selectively permeable membrane separating the compartments, provides many attributes relative to traditional devices. It can be configured for high-density cell culture, co-culture, and sample dialysis while rolling or standing still. It can also be configured for continuous movement of liquid between compartments. The wide combination of attributes not found in other membrane based cell culture and bioprocessing devices includes more cell capacity, more cell secreted product capacity, higher cell and product density, increased medium capacity, minimized use of exogenous growth factors, compatibility with standard cell culture equipment and protocols, increased scale up efficiency, capacity to function when rolling or standing still, capacity for perfusion without the need for pumps, and more efficient sample dialysis.
Owner:WHEATON INDS

Culture substrate, culture sheet, and cell culture method

Disclosed is a culture sheet which enables technology in which three-dimensional tissues with uniform diameter are formed without applying chemicals to the surface of a culture substrate. A plurality of holes are formed on the culture sheet of the culture substrate, and nanopillars capable of controlling the adhesiveness or migration of a cell are formed on a culture surface that serves as the bottom surface of each of the holes. The culture surface of each of the holes having a structure in which a partition wall is provided, wherein, by forming the internal nanopillars in the vicinity of the center of each of the holes, the interaction of the disseminated cells can be limited to uniform the size of the three-dimensional structures of the cells to be formed.
Owner:HITACHI LTD

Method for cell culture

A method for stem or progenitor cell culture. More precisely, the invention relates to a method for cell culture using one or more IαI (inter-alpha trypsin inhibitor or Inter-alpha inhibitor) protein(s) or part(s) thereof as a component in a cell culture media or a coating on a cell culture surface material. Furthermore the invention relates to a cell culture media and a cell culture coating / matrix provided with one or more IαI proteins(s) or part(s) thereof.
Owner:CYTIVA SWEDEN AB

A culture method of immune cells

The invention discloses a culture method of immune cells, comprising the following steps: (1) collecting peripheral blood mononuclear cells; (2) inoculating the cells into a culture flask containing aculture medium for culture; (3) introducing the cells cultured in the step (2) into a culture bag, wherein the volume of the culture liquid is smaller than the volume of the culture bag; (4) Harvestimmune cells. The invention provides a culture method of immune cells, by controlling the proportion of the culture liquid volume and the volume of the culture bag in the culture process, the expansion ability of cells and the number of cells finally obtained are improved, the proliferation of immune cells is realized in large quantities, the utilization rate of the culture solution is improved, and the cost is saved.
Owner:HENAN CANCER HOSPITAL

Microbeads for cell culture and method of monitoring cell culture using the same

Disclosed are microbeads for cell culture and a method of monitoring cell culture using the same. More particularly, each of the microbeads for cell culture according to an embodiment of the present invention include a core and a surface modification layer formed on a surface of the core. By using the method of monitoring cell culture with the microbeads for cell culture according to an embodiment of the present invention, cell culture may be carried out in highly scaled-up dimension and easily monitored.
Owner:UNIV IND COOP GRP OF KYUNG HEE UNIV

Insect cell culture medium

The invention belongs to the technical field of in vitro cell culture, and relates to an insect cell culture medium. The insect cell culture medium is prepared from carbohydrate, amino acid, inorganic salt, vitamins, yeast extract, lactoalbumin hydrolysate, cholesterol, linoleic acid, malic acid, fumaric acid, soyabean lecithin, glutamine, putrescine and 0.1 to 1 g / L of glycerinum. The insect cell culture medium is scientific in matching of various components, so that the living time of insect cells can be effectively prolonged, the cell can still have sufficient nutrition under the condition that the culture medium is not replaced for a long time, and the metabolic balance is maintained; and in addition, the culture medium contains fewer types of components and is simple in preparation and low in cost.
Owner:严志海

Cell culture device and method for simulating microgravity-compressive stress joint stimulation

The invention provides a cell culture device and a cell culture method for simulating microgravity-compressive stress joint stimulation. The device comprises a cell culture unit, a pressure monitoringunit, a microgravity simulation unit and a control unit, wherein the pressure monitoring unit and the microgravity simulation unit are arranged on the cell culture unit, and are electrically connected with the control unit. According to the device and the method, pressure in a culture bottle and the rotational microgravity of the culture bottle are controlled for cell culture researches under thecondition of coaction of different pressure stresses such as normal pressure, high pressure or low pressure and a simulated microgravity on cells as well as mechanical effect cell researches in caseof independent action of the normal pressure, the high pressure, the low pressure or the simulated microgravity.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY

Cell culture method and cell culture plate applied thereto

The invention provides a cell culture method and a cell culture plate applied thereto. The cell culture plate is characterized in that a stirring shaft is perpendicularly fixed in each deep culture hole of the cell culture plate; blades are fixed to each stirring shaft; the height of each blade is smaller than that of each hole; under the action of a certain external rotating speed, the cell culture plate and the blades synchronously move; the blades rotate relative to a culture solution and are used for stirring and mixing the culture solution in the cell culture plate. The volume dissolved oxygen coefficient of the cell culture plate can be increased and is approximate to that of a fermentation tank.
Owner:成都英德生物医药设备有限公司 +1

Immobilized cell culture method

ActiveCN104974976AIncrease productionAvoid or alleviate growth restrictionTissue culture3D cell cultureCell layer
The invention discloses an immobilized cell culture method, relating to the technical field of cell culture, for increasing media obtained by superficial cells in cellular layers within unit time, so as to improve the yield of immobilized culture of the cells. The immobilized cell culture method comprises the following steps: inoculating the cells onto a support layer, so as to form a cell layer attached to the support layer; and supplying media to the support layer, wherein the media wet the support layer and the cell layer, a plurality of first channels are formed in the cell layer, and the media are stored in the first channels. The immobilized cell culture method provided by the invention is used for culturing of algae, fungi and bacteria.
Owner:ENN SCI & TECH DEV

Cell culture in culture media free of components of animal origin

The present invention relates to a process for animal, preferably human, diploid anchorage-dependent cell culture, in the absence of exogenous components of primary animal origin, and to a cell culture medium substantially free of exogenous components of primary animal origin suitable for carrying out said process. In particular the invention concerns a cell culture medium which comprises at least one, more preferably several, exogenous animal-free growth factors. The present invention also relates to a process for cultivating animal, preferably human diploid anchorage-dependent cells in a medium according to the invention, involving the use of a trypsin substitute of non-animal origin for passaging cells. The invention further relates to a process for producing viruses, viral vaccines and the like.
Owner:GLAXOSMITHKLINE BIOLOGICALS SA

Cell culture method and medium for preparing human interferon betala and application of cell culture medium

ActiveCN102533658ASolve the problem of low culture yield and low activityMicroorganism based processesForeign genetic material cellsCell culture mediaElisa method
The invention relates to the field of biological medicine, in particular to a cell culture method and medium for preparing human interferon betala and application of the cell culture medium. According to the technical scheme of the invention, the cell culture medium for preparing the human interferon betala is provided, and the ingredients of the cell culture medium consist of a basal culture medium and additives, wherein the concentration of the basal culture medium is 12-18 g / L, and the additives contain the following ingredients of: D-galactose with the concentration of 0.05-0.45 g / L, D-mannitol with the concentration of 0.3-0.7 g / L, N-acetyl glucosamine with the concentration of 0.10-0.50 g / L, and D-glucose with the concentration of 1.0-5.0 g / L. According to the serum-free culture medium and method, the problems of low yield and low activity in the cell culture of the recombinant human interferon betala, are solved, the biological activity of the interferon betala in the cell collection liquid finally obtained by the cell culture method reaches 1*10<6> IU / ml, and the content of the interferon betala reaches over 0.8 g / L through the detection of an ELISA method.
Owner:深圳未名新鹏生物医药有限公司 +1

Isolated culture method for cartilage cells

The invention relates to the technical field of cell culture, in particular to an isolated culture method for cartilage cells. The isolated culture method comprises the following steps: treating a cartilage tissue into tissue blocks, adding trypsin for digestion, and then adding type II collagenase for digestion, wherein the concentration by mass percentage of the type II collagenase is 0.05 to 0.1 percent, and the digestion time of the type II collagenase is 16 to 20 hours. An experimental research shows that by the adoption of proper concentration and digestion time of type II collagenase for digestion of the cartilage tissue, the vitality of the cartilage cells can be effectively retained, so that enough number of cartilage cells can be obtained within relatively short time during later multiplication culture of the cartilage cells obtained by separation, and the cell culture efficiency is greatly improved.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD

Cell culturing and/or biomanufacturing system

The present invention relates to systems and methods for optimising the usage of laboratory and cell culturing space for cell culture and biomanufacturing. The invention provides a system and method that can be used to provide a plurality of workstations and / or storage bays for bioreactors in cell culturing and / or biomanufacturing facilities.
Owner:GLOBAL LIFE SCI SOLUTIONS OPERATIONS UK LTD

Cell culture medium

A cell culture medium with high content of choline chloride is provided. The cell culture media further comprise only moderate amounts of amino acids, in particular the amount of glutamine in the cell culture media is limited. The cell culture media can be used for large scale production of polypeptides using cell cultures. The cell culture media with high content of choline chloride are particularly suitable for fed-batch cell culture whereby cell viabilities stay at a higher level for a longer time and high polypeptide titers although limited amounts of amino acids are used.
Owner:NOVARTIS AG

Skeletal muscle cell in-vitro efficient culture method

The invention provides a skeletal muscle cell in-vitro efficient culture method, and belongs to the field of cell culture. The method includes the steps of preparing a test tool, extracting a skeletal muscle, digesting the skeletal muscle, preparing skeletal muscle suspension, and culturing the skeletal muscle suspension. The method is direct and effective when fibroblasts are removed through a differential iron wall method, the mixing of surface enveloped cells and bacteria is ingeniously avoided, cell and bacteria contamination is reduced, and meanwhile the purity of target cells is greatly improved and can approach 100% during cell separation. By means of the method, high-purity, high-survival-rate and high-activity skeletal muscle cells extremely low in contamination risk can be obtained, the cultured cells are good in differentiation and high in cell activity, and the method has great popularization significance.
Owner:杨慧慧

Cell culture method

The invention relates to the technical field of biology, and discloses a cell culture method. The cell culture method disclosed by the invention can homogenize nutrient substances and metabolites of cells in the tank body of a fluidized bed bioreactor, so that the cell density can reach higher than 10<7> / ml, thereby implementing continuous culture. The cell culture method disclosed by the invention is free from the restrictions of conditions, and has great scale-up potential and wide industrial application prospects.
Owner:SHANGHAI RITAI MEDICINE EQUIP PROJECT

Scaffold material with function of delaying cell aging and applications thereof

The invention discloses a scaffold material with the function of delaying cell aging and applications thereof. The scaffold material is a polylactic acid-caprolactone scaffold material grafted with resveratrol. The characterization and cell culture research on the scaffold material provided by the invention show that the modified scaffold material not only overcomes the defects that the hydrophobic scaffold body and too-small pores are beneficial to cell growth due to the addition of hydrophilic groups, but also can obviously delay cell aging. The material is simple and easy in modification method, has obvious effect, is used for nourishing liver seed cells, has the effects of delaying cell aging and improving seed cell activity, and lays a good foundation for liver tissue engineering preparation. The invention provides a new method for solving the problem of cell life in liver tissue engineering research.
Owner:SOUTH CHINA NORMAL UNIVERSITY

A kind of support material and its application with the effect of delaying cell aging

The invention discloses a scaffold material capable of delaying cell aging and an application thereof. The scaffold material is a polylactic acid-caprolactone scaffold material grafted with resveratrol. The characterization and cell culture studies of the scaffold material provided by the present invention show that the modified scaffold material not only overcomes the defects of hydrophobicity of the scaffold body and too small pores that are not conducive to cell growth due to the increase of hydrophilic groups, but also the material can significantly Delay cellular aging. The modification method of the material is simple and easy, and the effect is remarkable. It is used for nourishing liver seed cells, has the effect of delaying cell aging, improving the vitality of seed cells, and lays a good foundation for the preparation of liver tissue engineering. Provide a new method for solving the cell lifespan problem in liver tissue engineering research.
Owner:SOUTH CHINA NORMAL UNIVERSITY

Optimizing pumping of variable viscosities via microtextured miniaturized tesla pump

An integrated flow source is a limiting factor in numerous microfluidic applications. In addition to precise gradients and controlling molecular transports, a built-in source of stable and accurate flow can enable novel shear stress modulations for long-term cell culturing studies. The Tesla turbine, when used as a pump on the microfluidic regime, produces stable and accurate fluid gradients by utilizing laminar flow between its rotating discs Utilizing a stereolithography based 3D printer, a tesla pump (Ø10 cm) and associated housing capable of driving a microfluidic gradient is provided having a printed rotor surface topology of the pump in order to enhance pumping of biological fluids like blood at elevated viscosities. The surface topology is tuned via 3D pixilation, and this modulation completely recovered the pressure loss between pumping water at 1 cP versus glycerol solution at 3 cP. As a result, increased fluid viscosities, and even Non-Newtonian viscosities, can be used.
Owner:RGT UNIV OF MICHIGAN

A cell co-culture chip

The invention discloses a cell coculture chip. The cell coculture chip comprises a shell, a carrier, a micro structure and a perfusion system, wherein the shell and the carrier are sealed, the micro structure is arranged on the inner surface of the shell, the micro structure comprises a nutrient solution perfusion unit, a plurality of cell culture units and a biogel perfusion unit, and the perfusion system comprises a solution feeding column, a nutrient solution perfusion unit and a negative pressure suction device which are sequentially connected. By adopting the microfluidic chip, multiple cell ingredients are concentrated on one miniature chip for carrying out coculture, biological factors produced by multiple cells can diffuse to the whole miniature platform, and heterogeneous cells are out of touch each other, so that a growth microenvironment of an isolated cell in vivo is simulated to the utmost extent.
Owner:THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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