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Insect cell culture medium

A technology of insect cells and culture medium, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve problems such as decline, aging or death, and achieve the effect of maintaining high vitality survival, simple preparation, and scientific matching.

Inactive Publication Date: 2017-06-13
严志海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although there are many commercial insect serum-free mediums, the cells will gradually decline, age or die in the serum-free medium from a few generations to a dozen generations, so each serum-free medium have their own characteristics and limitations, and need to be further optimized

Method used

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  • Insect cell culture medium
  • Insect cell culture medium
  • Insect cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An insect cell culture medium consists of the following components and parts:

[0027] Maltose 20g / L, yeast extract 10g / L, hydrolyzed milk protein 3g / L, cholesterol 1ml / L, linoleic acid 1ml / L, malic acid 0.4g / L, fumaric acid 0.1g / L, soybean lecithin 0.1g / L , glutamine 0.1g / L, putrescine 0.1g / L and glycerin 0.1g / L.

[0028] Amino acid composition, as shown in Table 1 below:

[0029] Amino acid composition in the medium of table 1

[0030] arginine 1.32g / L Leucine 1.0g / L Isoleucine 0.1g / L aspartic acid 2.45g / L glutamic acid 0.29g / L Lysine 0.1g / L threonine 0.46g / L proline 0.19-0.95g / L Glycine 0.02g / L Phenylalanine 1.38g / L Methionine 1.38g / L

[0031] Vitamin composition, as shown in Table 2:

[0032] Vitamin composition in the medium of table 2

[0033] Niacinamide 0.10mg / L Pyridoxine 0.25mg / L pantothenic acid 0.23mg / L biotin 0.35mg / L riboflavin ...

Embodiment 2

[0038] An insect cell culture medium consists of the following components and parts:

[0039] Glucose 50g / L, yeast extract 30g / L, hydrolyzed milk protein 10g / L, cholesterol 20ml / L, linoleic acid 1ml / L, malic acid 1.8g / L, fumaric acid 0.8g / L, soybean lecithin 0.1g / L , glutamine 0.1g / L, putrescine 0.1g / L and glycerin 0.1g / L.

[0040] Amino acid composition, as shown in Table 4 below:

[0041] Amino acid composition in the medium of table 4

[0042] arginine 5.36g / L Leucine 2.56g / L Isoleucine 2.42g / L aspartic acid 4.27g / L glutamic acid 1.25g / L Lysine 3.67g / L threonine 1.64g / L proline 0.95g / L Glycine 0.65g / L Phenylalanine 5.35g / L Methionine 6.69g / L

[0043] Vitamin composition, as shown in table 5:

[0044] Vitamin composition in the medium of table 5

[0045] Niacinamide 0.58mg / L Pyridoxine 1.38mg / L pantothenic acid 2.41mg / L biotin 0.78mg / L riboflavin ...

Embodiment 3

[0051] An insect cell culture medium consists of the following components and parts:

[0052]Fructose 33g / L, yeast extract 15g / L, hydrolyzed milk protein 8g / L, cholesterol 13ml / , linoleic acid 7ml / L, malic acid 1.69g / L, fumaric acid 0.63g / L, soybean lecithin 16g / L, gluten Aminoamide 0.8g / L, putrescine 1.6g / L and glycerol 0.7g / L.

[0053] Amino acid composition, as shown in Table 7 below:

[0054] Amino acid composition in the medium of table 7

[0055] arginine 2.46g / L Leucine 1.35g / L Isoleucine 1.78g / L aspartic acid 3.56g / L glutamic acid 0.97g / L Lysine 2.98g / L threonine 1.03g / L proline 0.83g / L Glycine 0.54g / L Phenylalanine 4.63g / L Methionine 3.21g / L

[0056] Vitamin composition, as shown in Table 8:

[0057] Vitamin composition in the medium of table 8

[0058] Niacinamide 0.44mg / L Pyridoxine 0.89mg / L pantothenic acid 1.03mg / L biotin 0.52mg / L ribofla...

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PUM

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Abstract

The invention belongs to the technical field of in vitro cell culture, and relates to an insect cell culture medium. The insect cell culture medium is prepared from carbohydrate, amino acid, inorganic salt, vitamins, yeast extract, lactoalbumin hydrolysate, cholesterol, linoleic acid, malic acid, fumaric acid, soyabean lecithin, glutamine, putrescine and 0.1 to 1 g / L of glycerinum. The insect cell culture medium is scientific in matching of various components, so that the living time of insect cells can be effectively prolonged, the cell can still have sufficient nutrition under the condition that the culture medium is not replaced for a long time, and the metabolic balance is maintained; and in addition, the culture medium contains fewer types of components and is simple in preparation and low in cost.

Description

technical field [0001] The invention belongs to the technical field of in vitro cell culture, and relates to an insect cell culture medium. Background technique [0002] Cell culture is the propagation and cultivation of cells in vitro. This work began in the early 20th century and has now become an indispensable tool in the field of biological and medical research, and has developed into one of the important basic sciences. [0003] In the last 10 years, invertebrate cell and tissue culture have played an increasingly important role in the basic research of cell and molecular biology. Eukaryotic expression systems using baculovirus expression vectors and insect cultured cells as hosts have been established. It is widely used in the production of exogenous proteins, especially after the successful expression of a large number of active proteins with high medical value, it is favored by people. [0004] In recent years, serum-free cell culture technology has developed rapidl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07
CPCC12N5/0601C12N2500/05C12N2500/10C12N2500/12C12N2500/22C12N2500/30C12N2500/32C12N2500/34C12N2500/35C12N2500/36C12N2500/38C12N2500/74C12N2501/998
Inventor 严志海
Owner 严志海
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