Skeletal muscle cell in-vitro efficient culture method

Abstract

The invention provides a skeletal muscle cell in-vitro efficient culture method, and belongs to the field of cell culture. The method includes the steps of preparing a test tool, extracting a skeletal muscle, digesting the skeletal muscle, preparing skeletal muscle suspension, and culturing the skeletal muscle suspension. The method is direct and effective when fibroblasts are removed through a differential iron wall method, the mixing of surface enveloped cells and bacteria is ingeniously avoided, cell and bacteria contamination is reduced, and meanwhile the purity of target cells is greatly improved and can approach 100% during cell separation. By means of the method, high-purity, high-survival-rate and high-activity skeletal muscle cells extremely low in contamination risk can be obtained, the cultured cells are good in differentiation and high in cell activity, and the method has great popularization significance.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to a method for efficiently culturing skeletal muscle cells in vitro. Background technique [0002] Skeletal muscle, also known as striated muscle, is a type of skeletal muscle attached to bones. Skeletal muscle cells are fibrous, not branched, with obvious striations and many nuclei, all of which are located below the cell membrane. In skeletal muscle cells, there are many thin filamentous myofibrils arranged in parallel along the long axis of the cells. Each myofibril has alternate bright bands (I bands) and dark bands (A bands). The bright bands are lighter and the dark bands are darker. There is a brighter line in the middle of the dark band called the H line. There is an M line in the middle of the H line. In the middle of the bright band, there is a darker line called the Z line. The section between the two Z lines is called a sarcomere, about 1.5 to 2.5 microns l...

AI Technical Summary

Problems solved by technology

However, the existing clinical trials did not realize a problem: the cell culture technology used could not maintain the regenerative ability of skeletal muscle stem cells

When such cells are injected into the patient's body, it is naturally impossible to effectively generate new skeletal muscle fibers, and naturally cannot achieve the envisioned therapeutic effect.