Culture substrate, culture sheet, and cell culture method

Abstract

Disclosed is a culture sheet which enables technology in which three-dimensional tissues with uniform diameter are formed without applying chemicals to the surface of a culture substrate. A plurality of holes are formed on the culture sheet of the culture substrate, and nanopillars capable of controlling the adhesiveness or migration of a cell are formed on a culture surface that serves as the bottom surface of each of the holes. The culture surface of each of the holes having a structure in which a partition wall is provided, wherein, by forming the internal nanopillars in the vicinity of the center of each of the holes, the interaction of the disseminated cells can be limited to uniform the size of the three-dimensional structures of the cells to be formed.

Description

technical field

[0001] The present invention relates to techniques for culturing animal and plant cells using culture equipment to form spherical tissues (three-dimensional tissues) and monolayer tissues (two-dimensional planar tissues) of cells. Background technique

[0002] In the process of drug development, in vitro (in vitro) analysis using cells is required as an alternative to animal experiments. In particular, the screening of candidate drugs and the trend of toxicity and metabolism tests have become active.

[0003] Based on this background, many studies using cell replacement methods that replace existing animal experiments have been attempted, but they are often limited in predicting clinical responses. The reason for this is considered to be that cells do not adopt a structure that mimics an actual in vivo structure in these culture methods (Non-Patent Document 1). Therefore, attempts have been made to construct three-dimensional tissues that function closer to...

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