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A culture method of immune cells

A technology of immune cells and culture methods, which is applied in the field of immune cell culture, can solve the problems of reducing the culture medium efficiency, limited number of immune cells, poor effect, etc., and achieves the goal of improving expansion ability, realizing large-scale proliferation, and saving costs Effect

Pending Publication Date: 2019-01-15
HENAN CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method increases the number of cells to a certain extent, it seriously reduces the culture efficiency of the culture medium, and the number of immune cells finally obtained is very limited, and the effect is not good.

Method used

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  • A culture method of immune cells
  • A culture method of immune cells
  • A culture method of immune cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Induction of CIK cells

[0022] (1) Take 50ml of the patient’s peripheral blood and add heparin sodium for anticoagulation, divide it into two 50ml centrifuge tubes for centrifugation, centrifugal force 800G, 4℃, acceleration 9, deceleration 9, centrifugation for 15 minutes, after centrifugation, suck the upper plasma and place it in Store at 4°C; dilute the blood cells in the two centrifuge tubes to 35ml with physiological saline respectively, and slowly add them to the centrifuge tube containing 15ml of lymphocyte separation solution for centrifugation, centrifugal force 800G, acceleration 4, deceleration 4, 20°C Centrifuge for 17 minutes. After centrifugation, the peripheral blood mononuclear cells are collected and placed in a 50ml centrifuge tube, diluted to 50ml with normal saline and placed in a centrifuge for centrifugation. Centrifugal force is 300G, acceleration 7, deceleration 5, and 4℃ centrifugation for 8 minutes. Washes

[0023] (2) Inoculate the was...

Embodiment 2

[0025] Example 2 Induction and culture of CIK cells

[0026] The difference between Example 2 and Example 1 is that the volume of the culture solution in each culture bag in the above step (3) is 900 ml, and the ratio of the volume of the culture solution to the volume of the culture bag is 0.5:1.

Embodiment 3

[0027] Example 3 Induction of CIK cells

[0028] The difference between Example 3 and Example 1 is: in the above step (3), the volume of the culture solution in each culture bag is 990 ml, and the ratio of the volume of the culture solution to the volume of the culture bag is 0.55:1.

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Abstract

The invention discloses a culture method of immune cells, comprising the following steps: (1) collecting peripheral blood mononuclear cells; (2) inoculating the cells into a culture flask containing aculture medium for culture; (3) introducing the cells cultured in the step (2) into a culture bag, wherein the volume of the culture liquid is smaller than the volume of the culture bag; (4) Harvestimmune cells. The invention provides a culture method of immune cells, by controlling the proportion of the culture liquid volume and the volume of the culture bag in the culture process, the expansion ability of cells and the number of cells finally obtained are improved, the proliferation of immune cells is realized in large quantities, the utilization rate of the culture solution is improved, and the cost is saved.

Description

Technical field [0001] The invention relates to the field of cell culture, in particular to a method for culturing immune cells. Background technique [0002] At present, malignant tumor is a disease that seriously endangers human health and is one of the main causes of death. Its occurrence and development are mainly due to the loss of regulation and control of cancer cells by the body's defense system, resulting in an imbalance between the body and the tumor. . Traditional treatment methods include surgery, radiotherapy, and chemotherapy. Studies have shown that surgery, radiotherapy, and chemotherapy can cure tumors not by killing all tumor cells, but because when the tumor load is significantly reduced, the body’s immune function is restored. Thereby, small lesions are eliminated or the proliferation of residual tumor cells is significantly inhibited. Tumor immunotherapy is to stimulate and mobilize the body's immune system through human intervention, enhance anti-tumor imm...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N5/0646C12N2501/20
Inventor 郭金东高全立张成娟李铁鹏
Owner HENAN CANCER HOSPITAL
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