Cell culture device and cell culture method

Abstract

The invention discloses a cell culture device. The cell culture device comprises a control system, a cold closet, a liquid adding mechanism, a first centrifugal machine, a second centrifugal machine, a plasma preparation device, an incubator, a video viewing system and a laminar cleaning system which are integrated in a machine body. The plasma preparation device is integrated in the cell culturedevice and is used for activating plasma to obtain activated plasma; when the cell culture is performed, the activated plasma is added during the induction period and the growth culture period; and because the activated plasma is homologous with cultured cells, the cultured cells are easier to accept culture environment, so that the yield of the cultured cells is improved. In addition, various components of the cell culture device are all integrated together, no special spatial environment and place is needed, the whole process is entirely and automatically executed by the device, and the contact of human bodies is avoided, so that the chance of operation pollution is reduced, the cleanliness during the cell culture is furthest guaranteed, and the quality of the cultured cells can be improved.

Description

technical field [0001] The invention relates to cell culture in vitro, in particular to a cell culture device and method for large-scale automatic cell culture. Background technique [0002] Malignant tumors are currently one of the major diseases that endanger human health. In 1990, malignant tumors had become the disease with the highest fatality rate in China. By 2008, 153.6 people per 100,000 people died of malignant tumors, accounting for The fatality rate is 27.12%, which is higher than 19.65% and 19.62% of heart disease and cerebrovascular disease. At present, despite radical surgical resection, combined with radiation therapy and chemotherapy, nearly 50% of cancer patients die of postoperative recurrence and metastasis. Although radiotherapy and chemotherapy can act on tumors that cannot be treated by surgery, they both have serious side effects and cause great damage to body tissues, and some tumor cells are not sensitive to them, so the curative effect is not idea...

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Problems solved by technology

[0004] However, the cultivation of anti-cancer immune cells is different from drug production, and immune cells cannot be sterilized, so a higher aseptic operating environment and aseptic operating techniques are required

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