A cell co-culture chip

Abstract

The invention discloses a cell coculture chip. The cell coculture chip comprises a shell, a carrier, a micro structure and a perfusion system, wherein the shell and the carrier are sealed, the micro structure is arranged on the inner surface of the shell, the micro structure comprises a nutrient solution perfusion unit, a plurality of cell culture units and a biogel perfusion unit, and the perfusion system comprises a solution feeding column, a nutrient solution perfusion unit and a negative pressure suction device which are sequentially connected. By adopting the microfluidic chip, multiple cell ingredients are concentrated on one miniature chip for carrying out coculture, biological factors produced by multiple cells can diffuse to the whole miniature platform, and heterogeneous cells are out of touch each other, so that a growth microenvironment of an isolated cell in vivo is simulated to the utmost extent.

Description

technical field [0001] The invention relates to a chip suitable for joint culture of various cells, which belongs to the field of biomedical research. Background technique [0002] Cell biology research is developing from the observation and study of a single cell to the observation and study of the interaction of two or even multiple types of cells. For complex biological systems, a system in which multiple cells exist at the same time can better simulate the real cellular microenvironment, thereby more comprehensively and deeply exploring the complex network between cells and the signaling pathways that mediate their interactions, etc. . Cell co-cultivation is a common method for studying cell-cell interactions. [0003] At present, although the conventional mixed culture of cells can solve the problem of multi-cell interaction in vitro, and at the same time play a role in simulating the in vivo environment, at present, the microfluidic chip based on cell culture is most...

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Problems solved by technology

[0003] At present, although the conventional mixed culture of cells can solve the problem of multi-cell interaction in vitro, and at the same time play a role in simulating the in vivo environment, at present, the microfluidic chip based on cell culture is mostly limited to the combination of two types of cells. Culture, or a kind of target cell mixed with other kinds of cells. In this state, the phenotype of the isolated cells is greatly different from that before the in vitro, which limits the observation of each cell component in the microenvironment. For the cells in this state Sensitivity testing of chemotherapy drugs also limits the accuracy of the results

In addition, the perfusion channel of the culture chamber of the microfluidic chip currently used for drug screening is mostly two relatively independent and parallel channels, which limits the contact area between the channel and the cell culture chamber; the perfusion of the cell nutrient solution is mostly maintained by a micropump. Limiting the large-scale application in clinical

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