Immobilized cell culture method

Abstract

The invention discloses an immobilized cell culture method, relating to the technical field of cell culture, for increasing media obtained by superficial cells in cellular layers within unit time, so as to improve the yield of immobilized culture of the cells. The immobilized cell culture method comprises the following steps: inoculating the cells onto a support layer, so as to form a cell layer attached to the support layer; and supplying media to the support layer, wherein the media wet the support layer and the cell layer, a plurality of first channels are formed in the cell layer, and the media are stored in the first channels. The immobilized cell culture method provided by the invention is used for culturing of algae, fungi and bacteria.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a method for immobilized culture of cells. Background technique [0002] Cell immobilization culture is a relatively common cell culture method. This cell culture method has the advantages of low cost, low energy consumption, and high yield per unit culture area. It is suitable for the cultivation of algae, bacteria and fungi. [0003] Generally, in the immobilized culture of cells, first cells are seeded on the support layer to form a cell layer attached to the support layer (the cell layer includes surface cells farther from the support layer and bottom cells closer to the support layer), Then provide the medium to the support layer, so that the medium infiltrates the support layer and the cell layer on it, and at the same time provide light and carbon dioxide and other gases to the cell layer, so that the cells in the cell layer can grow, thereby realizing the immobilized...

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Problems solved by technology

[0004] However, in the above culture process, if the culture medium is added from the surface of the cell layer, the surface of the cell layer will be washed away. For cells with insufficient adhesion between each other, the culture medium will wash away the cells on the surface of the cell layer, and then Affect yield

Therefore, on the support layer, the medium is usually provided to the surface opposite to the surface to which the cells are attached, to avoid the problem of medium scouring the surface cells, but at this time the medium must pass through the support layer and the bottom cells in the cell layer to be able to The superficial cells that penetrate into the cell layer cause the medium to not penetrate the support layer and the underlying cells unimpededly and finally provide to the superficial cells, resulting in the restriction of the growth of the superficial cells and the reduction of the medium obtained by the superficial cells per unit time, Finally, the yield of immobilized culture of cells is reduced

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