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Cell culture method and cell culture plate applied thereto

A technology of cell culture and culture solution, which is applied in the field of cell culture plates, can solve problems such as inability to accurately predict the results of aerobic cell culture, small gas-liquid phase surface of cell culture plates, and complex structure of small fermentation tanks, and achieve simple structure and high efficiency. Mixing effects, effects that induce violent flipping

Active Publication Date: 2014-03-05
成都英德生物医药设备有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The structure of the cell culture plate is simple and easy to operate, and it is widely used in strain screening. During use, the culture plate is generally placed on an oscillator, and the oscillator provides a certain speed and eccentricity, so that the culture in the culture plate However, the gas-liquid phase surface of the cell culture plate is small, the liquid mixing effect is poor, and the volumetric oxygen transfer coefficient is significantly smaller than that of the fermenter, so that the scale-up based on the culture plate cannot accurately predict the good performance in the fermenter. The culture results of oxygen cells, at the same time, the strains screened by the culture plate are not necessarily suitable for the fermenter; at the same time, each small fermenter is equipped with a motor, which drives the paddle fixed at the output end to rotate to stir the culture medium. The volume oxygen transfer coefficient of the small fermenter is relatively close to that of the fermenter, but the structure of the small fermenter is complicated and the manufacturing cost is high, so its use is greatly restricted

Method used

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  • Cell culture method and cell culture plate applied thereto
  • Cell culture method and cell culture plate applied thereto
  • Cell culture method and cell culture plate applied thereto

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A cell culture method. The culture device is a cell culture plate. The cell culture plate is fixed on a shaker. Each culture deep hole of the cell culture plate is vertically fixed with a stirring shaft, and a paddle is fixed on the stirring shaft. Under the action of a certain rotation speed and eccentricity provided, the cell culture plate and the paddle move synchronously, and the paddle rotates relative to the culture liquid to stir and mix the culture liquid in the cell culture plate.

Embodiment 2

[0027] Such as Figure 1 to Figure 5 As shown, the cell culture plate is evenly distributed with 24 deep culture holes located in the axial direction of the deep culture hole, a stirring shaft is fixed on the bottom end surface of the deep culture hole, and the upper part of the stirring shaft is evenly fixed circumferentially Four paddles, two adjacent paddles are arranged staggered in the depth direction of the deep culture hole, and two opposite paddles are arranged in parallel at the same height in the depth direction of the deep culture hole Four baffles are fixed on the inner periphery of the deep culture hole, and the baffles are evenly distributed along the peripheral surface of the deep culture hole and are arranged at equal height in the depth direction of the deep culture hole.

Embodiment 3

[0029] It is basically the same as Example 2, except that the paddles are arranged at the same height in the depth direction of the deep culture hole.

[0030] Such as Image 6 Said, the cell culture plate is evenly distributed with 24 deep culture holes, located in the axial direction of the deep culture hole, a stirring shaft is fixed on the bottom end of the deep culture hole, and four holes are evenly fixed on the upper part of the stirring shaft in the circumferential direction. Four paddles are arranged at the same height in the depth direction of the deep culture hole; four baffles are fixed on the inner periphery of the deep culture hole, and the baffles are arranged along the circumference of the deep culture hole. The surfaces are evenly distributed and arranged at the same height in the depth direction of the deep culture hole.

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Abstract

The invention provides a cell culture method and a cell culture plate applied thereto. The cell culture plate is characterized in that a stirring shaft is perpendicularly fixed in each deep culture hole of the cell culture plate; blades are fixed to each stirring shaft; the height of each blade is smaller than that of each hole; under the action of a certain external rotating speed, the cell culture plate and the blades synchronously move; the blades rotate relative to a culture solution and are used for stirring and mixing the culture solution in the cell culture plate. The volume dissolved oxygen coefficient of the cell culture plate can be increased and is approximate to that of a fermentation tank.

Description

Technical field [0001] The invention relates to the technical field of cell culture devices, in particular to a cell culture method and a cell culture plate used in the same. Background technique [0002] High-throughput parallel fermentation technology is an advanced technology for studying fermentation processes, exploring fermentation control parameters, and screening dominant strains in a small system culture device. The research results can be directly scaled up to industrial scale, greatly reducing R&D costs and improving R&D efficiency . [0003] The current high-throughput parallel fermentation technology mainly uses cell culture plates or small fermentors for cell culture. The structure of the cell culture plate is simple and easy to operate. It is widely used in strain screening. Generally, the culture plate is placed on a shaker during use, and the shaker provides a certain speed and eccentricity to make the culture inside the plate The liquid oscillates and mixes, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/02
CPCC12M23/12C12M27/02
Inventor 白仲虎隋涌聂简琪孙杨
Owner 成都英德生物医药设备有限公司
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