Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cover structure of cell culture plate

A cell culture, plate cover technology, applied in tissue cell/virus culture devices, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problem of small volume dissolved oxygen coefficient, reduce protein product expression, limit Problems such as high-density culture of bacteria or cells, to prevent cross-contamination, achieve high-density culture, and prevent water volatilization

Active Publication Date: 2015-09-02
JIANGNAN UNIV +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing small system culture devices below the fermenter scale have a small volumetric oxygen dissolution coefficient, which limits the high-density culture of strains or cells, and reduces the expression of protein products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cover structure of cell culture plate
  • Cover structure of cell culture plate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The patent of the present invention takes the structure of the cover of the 24-well deep-well culture plate as an example for illustration. The structure of the patent of the present invention is not limited to the cover of the 24-well culture plate, and is also applicable to the cover of the 48-well deep-well culture plate and the cover of the 96-well The plate cover of the culture plate and the plate cover of the culture plate of other number of holes, similarly, the structure of the patent of the present invention is applicable to the plate cover of the culture plate of the hole position of different shapes.

[0015] Such as figure 1 , figure 2 As shown, the plate cover structure of the cell culture plate includes the plate cover 1, inside the plate cover 1, an antifouling film 2, a waterproof film 3 and a silica gel plate 4 are sequentially installed from top to bottom, and a number of culture plates are evenly distributed on the cell culture plate. Deep hole 9, w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a plate cover structure of a cell culture plate. The volume coefficient of dissolved oxygen of the cell culture plate can be improved, high-density cultivation is achieved, the expression quantity of a protein product is increased, and cross contamination of a culture solution can be effectively avoided. The plate cover structure of the cell culture plate comprises a plate cover, wherein an anti-fouling membrane, a waterproof membrane and a silica gel plate are orderly arranged inside the plate cover from top to bottom; a plurality of culture deep holes are evenly distributed on the cell culture plate. The plate cover structure is characterized in that a pipe body is inserted on the plate cover corresponding to each culture deep hole; the culture deep holes are communicated with the external part of the cell culture plate by the pipe body; the pipe body comprises a breather pipe, an exhaust pipe and a feeding pipe.

Description

technical field [0001] The patent of the present invention relates to the technical field of cell culture devices in biology, in particular to deep-well culture plates for cell culture, specifically the cover structure of the cell culture plates. Background technique [0002] With the development of molecular biology, the heterologous protein expression technology has gradually matured and been widely used. The expression of foreign proteins requires the selection of suitable expression hosts and expression elements, which requires the construction of a huge expression library, from which a better expression kit can be selected. The cell culture plate can use less culture medium for high-throughput cell culture and screening of strains, cell lines and expression libraries, and has the characteristics of fast, high-throughput, and cost-saving. In the process of studying the expression of foreign proteins, such as antibodies and enzymes, it is necessary to improve the efficie...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/04
CPCC12M23/38
Inventor 白仲虎隋涌孙杨聂简琪
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com