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Plate cover structure of cell culture plate

A cell culture and plate cover technology, applied in tissue cell/virus culture devices, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problem of reducing protein product expression, limiting bacterial species or high cell density Cultivation, small volume dissolved oxygen coefficient, etc., to achieve high-density cultivation, prevent water volatilization, and prevent cross-contamination

Active Publication Date: 2014-03-19
JIANGNAN UNIV +1
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  • Description
  • Claims
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Problems solved by technology

[0003] Existing small system culture devices below the fermenter scale have a small volumetric oxygen dissolution coefficient, which limits the high-density culture of strains or cells, and reduces the expression of protein products

Method used

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  • Plate cover structure of cell culture plate
  • Plate cover structure of cell culture plate

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Embodiment Construction

[0014] The patent of the present invention takes the structure of the cover of the 24-well deep-well culture plate as an example for illustration. The structure of the patent of the present invention is not limited to the cover of the 24-well culture plate, and is also applicable to the cover of the 48-well deep-well culture plate and the cover of the 96-well The plate cover of the culture plate and the plate cover of the culture plate of other number of holes, similarly, the structure of the patent of the present invention is applicable to the plate cover of the culture plate of the hole position of different shapes.

[0015] Such as figure 1 , figure 2 As shown, the plate cover structure of the cell culture plate includes the plate cover 1, and inside the plate cover 1, an antifouling film 2, a waterproof film 3 and a silica gel plate 4 are sequentially installed from top to bottom. Deep hole 9, wherein, figure 2 Only one culture deep hole is shown in the figure, corresp...

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Abstract

The invention provides a plate cover structure of a cell culture plate. The volume coefficient of dissolved oxygen of the cell culture plate can be improved, high-density cultivation is achieved, the expression quantity of a protein product is increased, and cross contamination of a culture solution can be effectively avoided. The plate cover structure of the cell culture plate comprises a plate cover, wherein an anti-fouling membrane, a waterproof membrane and a silica gel plate are orderly arranged inside the plate cover from top to bottom; a plurality of culture deep holes are evenly distributed on the cell culture plate. The plate cover structure is characterized in that a pipe body is inserted on the plate cover corresponding to each culture deep hole; the culture deep holes are communicated with the external part of the cell culture plate by the pipe body; the pipe body comprises a breather pipe, an exhaust pipe and a feeding pipe.

Description

technical field [0001] The patent of the present invention relates to the technical field of cell culture devices in biology, in particular to deep-well culture plates for cell culture, specifically the cover structure of the cell culture plates. Background technique [0002] With the development of molecular biology, the heterologous protein expression technology has gradually matured and been widely used. The expression of foreign proteins requires the selection of suitable expression hosts and expression elements, which requires the construction of a huge expression library, from which a better expression kit can be selected. The cell culture plate can use less culture medium for high-throughput cell culture and screening of strains, cell lines and expression libraries, and has the characteristics of fast, high-throughput, and cost-saving. In the process of studying the expression of foreign proteins, such as antibodies and enzymes, it is necessary to improve the efficie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/04
CPCC12M23/38
Inventor 白仲虎隋涌孙杨聂简琪
Owner JIANGNAN UNIV
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