Cell culture method and cell culture plate applied thereto
A technology of cell culture and culture solution, which is applied in the field of cell culture plates, can solve problems such as inability to accurately predict the results of aerobic cell culture, small gas-liquid phase surface of cell culture plates, and complex structure of small fermentation tanks, and achieve simple structure and high efficiency. Mixing effects, effects that induce violent flipping
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Embodiment 1
[0025] A cell culture method, the culture device is a cell culture plate, the cell culture plate is fixed on the oscillator, each culture deep hole of the cell culture plate is vertically fixed with a stirring shaft, the stirring shaft is fixed with a paddle, and the oscillator Under the action of a certain rotational speed and eccentric distance provided, the cell culture plate and the paddles move synchronously, and the paddles rotate relative to the culture solution to stir and mix the culture solution in the cell culture plate.
Embodiment 2
[0027] like Figure 1 to Figure 5 As shown, the cell culture plate is evenly distributed with 24 deep culture wells, which are located in the axial direction of the deep culture wells. The bottom end surface of the deep culture wells is fixed with a stirring shaft, and the upper part of the stirring shaft is evenly fixed with Four paddles, two adjacent paddles are arranged in dislocation in the direction of the depth of the deep culture well, and the two opposite paddles are arranged in parallel at the same height in the direction of the depth of the deep culture well ; The inner periphery of the deep culture well is fixed with four baffles, the baffles are evenly distributed along the peripheral surface of the deep culture well, and are arranged at equal heights in the depth direction of the deep culture well.
Embodiment 3
[0029] It is basically the same as Example 2, except that the paddles are arranged at equal heights in the depth direction of the deep culture well.
[0030] like Image 6 Said, the cell culture plate is evenly distributed with 24 culture deep wells, located in the axial direction of the culture deep holes, the bottom end surface of the culture deep holes is fixed with a stirring shaft, and the upper part of the stirring shaft is evenly fixed with four Four paddles are arranged at the same height in the depth direction of the deep culture well; four baffles are fixed on the inner periphery of the deep culture well, and the baffles are arranged along the circumference of the deep culture well. The surface is evenly distributed and arranged at the same height in the depth direction of the deep culture well.
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