Deep well cell culture plate

Abstract

The invention provides a deep-hole cell culture plate, which can supply enough oxygen for cultivation of aerobic cells, so as to improve the dissolved oxygen coefficient of the culture plate. Culture deep holes are evenly distributed on the culture plate. The deep-hole cell culture plate is characterized in that gas passages are evenly arranged inside a bottom plate of the culture plate; air outlets communicated with each culture deep hole and air inlets communicated with the external part of the culture plate are arranged on the gas passages; a hollow air guide column is convexly arranged upwards on the bottom plate of the culture plate at the end pat of each air outlet.

Description

technical field [0001] The patent of the present invention relates to the technical field of cell culture devices, in particular to a deep-well cell culture plate for high-throughput and high-density culture of aerobic cells. Background technique [0002] The cell culture plate can use less culture medium for high-throughput cell culture and strain or cell strain screening, and has the characteristics of fast, high-throughput, cost-saving, etc. In the process of cell culture, the cell culture plate is generally used first High-volume, small-volume cell culture, screen out the optimal strains, and preliminarily determine the best process culture conditions (temperature, pH value, medium, etc.), and then combine the screened strains and the initially determined process The conditions are applied in a fermenter for scale-up cultivation. [0003] Oxygen is necessary for aerobic cell growth and is one of the most important factors in the scale-up process. Most fermenters are eq...

AI Technical Summary

Problems solved by technology

Most fermenters are equipped with stirring devices or oxygen supply devices. Fermenters can provide a higher volumetric oxygen dissolution coefficient during aerobic cell culture, while the existing deep-well cell culture plates simply provide a certain speed and eccentricity externally. Under the action of distance, the culture medium in the culture deep well oscillates, increases the contact area between the culture medium and the air, and improves the liquid mixing for oxygen supply. During the culture process, the gas-liquid phase surface is small, and the liquid mixing effect is poor, so that the culture plate provides The volume dissolved oxygen coefficient of the fermenter is significantly smaller than that of the fermenter. Due to the significant difference in the volume dissolved oxygen coefficient, the scale-up based on the culture plate cannot accurately predict the culture results in the fermenter. At the same time, the strains screened by the culture plate are not necessarily the best. suitable for fermenters

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