Preparation method of bionic injectable gelatin-silane composite medical hydrogel

Abstract

The invention discloses a preparation method of bionic injectable gelatin-silane composite medical hydrogel. The preparation method comprises the following steps: dissolving food and pharmaceutical grade gelatin powder in a phosphate buffer solution to obtain a gelatin solution; dissolving silane in a neutral phosphate buffer solution to obtain a silane solution; adding the silane solution into the gelatin solution according to a ratio of silane to gelatin of 0.5-2 ml to 1 g for carrying out a hydrolysis reaction to obtain transparent gelatin-silane composite sol; and gelatinizing the gelatin-silane composite sol at 37 DEG C. The bionic injectable gelatin-silane composite medical hydrogel disclosed by the invention contains no organic solvent or toxic component, and the formed composite medical hydrogel has high mechanical flexibility, maintains high stability in the phosphate buffer solution and has strong swelling resistance and approximately linear biodegradability; cell culture studies have shown that the bionic composite hydrogel prepared by the preparation method disclosed by the invention has good cell compatibility, thereby being capable of being applied to a variety of fields of soft tissue and bone tissue repair, tissue engineering and regeneration.

Description

technical field [0001] The invention relates to a preparation technology of bioactive biomimetic composite medical hydrogel material for bone, soft tissue repair, cell, protein and drug delivery, in particular to a preparation of biomimetic injectable gelatin-silane composite medical hydrogel method. Background technique [0002] Biomedical hydrogel has a three-dimensional network structure, highly hydrophilic and hydrating properties, and can load drugs, proteins, cells, and biomolecules. It is a class of superior tissue repair and biological carrier materials. It is in a liquid state at low temperature, and cross-linked into a gel at body temperature, which has a self-curing property, and has a wide range of applications in the field of minimally invasive medicine. [0003] Traditional injectable biomedical hydrogels include synthetic hydrogels and natural hydrogels, chemically cross-linked and physically cross-linked hydrogels, and synthetic hydrogels have poor biocompat...

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Problems solved by technology

[0005] Chinese Patent Publication No. CN1907504.0 (Injectable Hydrogel of Sodium Alginate Crosslinked Gelatin Containing Biphasic Calcium-Phosphorus Particles and Its Preparation Method and Application) and Chinese Patent Publication No. CN102219911A (An Injectable Polyphosphazene / Gelatin Composite hydrogel and its preparation method), through the analysis and comparison of the above research and inventions, it is found that the composition of the hydrogel described in patent CN1907504.0 is complex, the preparation cost is high, and the preparation process is complicated. At the same time, the patent uses a chemical cross-linking method, There is a possibility of potential toxicity; the polymer used in the same patent CN102219911A is relatively expensive, and the chemical cross-linking mechanism also has the disadvantage of poor biocompatibility

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