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Wheat stripe rust teliospore germination technology system

A technology of wheat stripe rust and teliospores, applied in the agricultural field, can solve problems such as the difficulty in germination of wheat stripe rust teliospores, and the lack of a suitable method for the germination of wheat stripe rust teliospores.

Inactive Publication Date: 2014-10-01
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difficulty in germination of teliospores of wheat stripe rust, for a long time, there has not been a method for the germination of teliospores of wheat stripe rust.

Method used

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Examples

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Effect test

Embodiment

[0009] A technology system for the germination of teliospores of wheat stripe rust. After the teliospore material of wheat stripe rust is treated at low temperature for 1 week or longer, the wheat tissue (leaf or leaf sheath) containing the teliospores of wheat stripe rust is treated with sterile water. ) soaked in water for 24 hours in a low-temperature environment, then removed and placed in an incubator at 16°C to dry naturally for 24 hours; repeat the above-mentioned dry-wet alternating steps for 2-4 cycles, and then soak the wheat stripe rust bacteria in sterile water The teliospore tissue of the teliospore is placed on 2-4% water agar medium for 24 hours, and cultured in an incubator at 16° C., and then the teliospore germination of the wheat stripe rust can be observed.

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Abstract

The invention relates to a wheat stripe rust teliospore germination technology system, which comprises the following steps: conducting low-temperature treatment on a wheat stripe rust teliospore material for one week or longer time, treating wheat tissues (leaves or sheathes) containing wheat stripe rust teliospore with sterile water, spraying water, soaking and placing in the low-temperature environment for 24 hours, then moving out and placing in a 16DEG C incubator and naturally drying for 24 hours; and repeating the above drying-wetting operation for 2-4 cycles, then soaking the wheat tissues containing wheat stripe rust teliospore for 24 hours with sterile water, placing the wheat stripe rust teliospore-containing tissue on a 2-4% water agar medium, placing in the 16DEG C incubator for culturing, and thus observing the germination of the wheat stripe rust teliospore. The wheat stripe rust teliospore germination technology system discloses that the wheat stripe rust in China has a perfect stage, and provides technological guarantee for disclosing the action of sexual propagation of the wheat stripe rust in China in the toxicity variation.

Description

technical field [0001] The invention relates to a teliospore germination technology system of wheat stripe rust, which belongs to the field of agricultural technology. Background technique [0002] Wheat stripe rust is the most serious wheat disease in my country. In the history of the prevalence of stripe rust, it has caused huge yield loss to my country's wheat production. At present, it is still the most destructive disease on wheat in my country. Race variation is the direct cause of the "loss" of wheat disease resistance and the occurrence of wheat stripe rust. The pathways of race variation include mutation, somatic recombination, and sexual reproduction. Teliospore germination of wheat stripe rust is the first step in sexual reproduction to study race variation. However, due to the difficulty in germination of teliospores of wheat stripe rust, for a long time, no suitable method for teliospore germination of wheat stripe rust has been mastered. The establishment o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 赵杰康振生黄丽丽
Owner NORTHWEST A & F UNIV
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