Method for artificial inoculation of insolated identification of white rust resistance of chrysanthemum
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A technology of artificial inoculation and white rust, applied in horticultural methods, botany equipment and methods, biochemical equipment and methods, etc., to achieve the effect of overcoming safety hazards
Inactive Publication Date: 2011-08-31
SHENYANG AGRI UNIV
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Problems solved by technology
So far, no method has been found to identify the resistance of chrysanthemum white rust through artificial in vitro inoculation using chrysanthemum stems cultivated in the field
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Examples
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Embodiment 1
[0018] Successful in vitro inoculation identified the resistance of the chrysanthemum variety 'Fenhuaxiaori' to chrysanthemum white rust, and identified the chrysanthemum variety 'Fenhuaxiaori' as being susceptible to chrysanthemum white rust.
[0019] The specific method of implementation is as follows:
[0020] A. Culture vessel preparation.
[0021] Use a 500ml wide-mouth glass bottle as an in vitro culture container. First wash the glass bottle with tap water, and then rinse it with distilled water 3 times.
[0022] B. Medium preparation and combination.
[0023] 1. The basic components of the medium: 1 / 2 the macroelement part of the MS medium.
[0024] The specific content is: ammonium nitrate (NH 4 NO 3 )825mg / L, potassium nitrate (KNO 3 )950mg / L, calcium chloride (CaCl 2 2H 2 O) 220mg / L, manganese sulfate (MgSO 4 ·7H 2 O) 185mg / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 850mg / L.
[0025] 2. Preparation method of culture medium
[0026] Preparation of l...
Embodiment 2
[0043] The successful in vitro inoculation identified the resistance of the chrysanthemum variety 'C029' to chrysanthemum white rust, and identified the resistance of the chrysanthemum variety 'C029' to chrysanthemum white rust.
[0044] The specific method of implementation is as follows:
[0045] A. In implementation, the specific methods of culture container preparation, medium preparation and combination, test chrysanthemum stem segment collection, pathogenic bacteria collection, pathogenic bacteria inoculation and cultivation are the same as in Example 1. The difference is that after 15 days of cultivation, no teliospore heaps appeared on the leaves of the tested chrysanthemum variety 'C029'.
[0046] The identification results showed that the chrysanthemum variety 'C029' was a disease-resistant variety.
[0047] B. Field testing for disease resistance identification.
[0048] 1. Experimental plot design
[0049] In the isolation area of the solar greenhouse, the hea...
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Abstract
The invention provides a method for artificial inoculation insolated identification of resistance of chrysanthemum to white rust of chrysanthemum. The method comprises the following steps: A, using a 500ml wide-mouth glass bottle as an insolated culture container; B, preparing a liquid culture medium and a solid culture medium respectively and combining the culture media by putting the liquid culture medium above the solid culture medium; C, cutting chrysanthemum stems which are healthy and not infected with pathogenic bacteria of chrysanthemum white rust; D, picking up chrysanthemum leaves with telia; E, rubbing teliospores on telia of infected leaves by hands, and inoculating the teliospores onto the chrysanthemum leaves to be detected; F, performing cottage of the chrysanthemum stems inoculated with the pathogenic bacteria in the culture bottle filled with the solid-liquid combined culture medium for culture by placing the chrysanthemum stems in the dark at 17 to 21 DEG C for 24 hours and then placing the chrysanthemum stems in light of which the intensity is 1,000 Lx for 16 hours and then in the dark in a culture room for 8 hours; and G, after 15 days, observing whether the telia is on the chrysanthemum leaves by eyes. The method for artificial inoculation insolated identification of white rust resistance of chrysanthemum provided by the invention has the advantages of eliminating potential safety hazards in identification of white rust resistance of chrysanthemum and realizing safe and effective identification of the resistance of different chrysanthemum varieties on white rust resistance of chrysanthemum under controlled conditions.
Description
technical field [0001] The invention belongs to the technical field of identification of flower disease resistance, in particular to a method for identifying the resistance of different chrysanthemum varieties to chrysanthemum white rust by artificial inoculation in vitro. Background technique [0002] Chrysanthemum (Dendranthema morifolium) is one of the top ten famous flowers in my country and one of the four fresh cut flowers in the world. It has important humanistic, economic and social values. Chrysanthemum white rust (CWR, chrysanthemum white rust) is a worldwide quarantine disease and one of the most important diseases that harm chrysanthemum. [0003] Chrysanthemum white rust is caused by Puccinia horiana Henn., a pathogen that mainly damages the leaves of chrysanthemums, with rapid onset and strong destructiveness. The disease was first discovered in Japan in 1895. It was first discovered in Shanghai in 1963 in my country, and then serious occurrences of the disea...
Claims
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Application Information
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