Method for artificial inoculation of insolated identification of white rust resistance of chrysanthemum
A technology of artificial inoculation and white rust, applied in horticultural methods, botany equipment and methods, biochemical equipment and methods, etc., to achieve the effect of overcoming safety hazards
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Embodiment 1
[0018] Successful in vitro inoculation identified the resistance of the chrysanthemum variety 'Fenhuaxiaori' to chrysanthemum white rust, and identified the chrysanthemum variety 'Fenhuaxiaori' as being susceptible to chrysanthemum white rust.
[0019] The specific method of implementation is as follows:
[0020] A. Culture vessel preparation.
[0021] Use a 500ml wide-mouth glass bottle as an in vitro culture container. First wash the glass bottle with tap water, and then rinse it with distilled water 3 times.
[0022] B. Medium preparation and combination.
[0023] 1. The basic components of the medium: 1 / 2 the macroelement part of the MS medium.
[0024] The specific content is: ammonium nitrate (NH 4 NO 3 )825mg / L, potassium nitrate (KNO 3 )950mg / L, calcium chloride (CaCl 2 2H 2 O) 220mg / L, manganese sulfate (MgSO 4 ·7H 2 O) 185mg / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 850mg / L.
[0025] 2. Preparation method of culture medium
[0026] Preparation of l...
Embodiment 2
[0043] The successful in vitro inoculation identified the resistance of the chrysanthemum variety 'C029' to chrysanthemum white rust, and identified the resistance of the chrysanthemum variety 'C029' to chrysanthemum white rust.
[0044] The specific method of implementation is as follows:
[0045] A. In implementation, the specific methods of culture container preparation, medium preparation and combination, test chrysanthemum stem segment collection, pathogenic bacteria collection, pathogenic bacteria inoculation and cultivation are the same as in Example 1. The difference is that after 15 days of cultivation, no teliospore heaps appeared on the leaves of the tested chrysanthemum variety 'C029'.
[0046] The identification results showed that the chrysanthemum variety 'C029' was a disease-resistant variety.
[0047] B. Field testing for disease resistance identification.
[0048] 1. Experimental plot design
[0049] In the isolation area of the solar greenhouse, the hea...
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