Method for artificial inoculation of insolated identification of white rust resistance of chrysanthemum

Abstract

The invention provides a method for artificial inoculation insolated identification of resistance of chrysanthemum to white rust of chrysanthemum. The method comprises the following steps: A, using a 500ml wide-mouth glass bottle as an insolated culture container; B, preparing a liquid culture medium and a solid culture medium respectively and combining the culture media by putting the liquid culture medium above the solid culture medium; C, cutting chrysanthemum stems which are healthy and not infected with pathogenic bacteria of chrysanthemum white rust; D, picking up chrysanthemum leaves with telia; E, rubbing teliospores on telia of infected leaves by hands, and inoculating the teliospores onto the chrysanthemum leaves to be detected; F, performing cottage of the chrysanthemum stems inoculated with the pathogenic bacteria in the culture bottle filled with the solid-liquid combined culture medium for culture by placing the chrysanthemum stems in the dark at 17 to 21 DEG C for 24 hours and then placing the chrysanthemum stems in light of which the intensity is 1,000 Lx for 16 hours and then in the dark in a culture room for 8 hours; and G, after 15 days, observing whether the telia is on the chrysanthemum leaves by eyes. The method for artificial inoculation insolated identification of white rust resistance of chrysanthemum provided by the invention has the advantages of eliminating potential safety hazards in identification of white rust resistance of chrysanthemum and realizing safe and effective identification of the resistance of different chrysanthemum varieties on white rust resistance of chrysanthemum under controlled conditions.

Description

technical field

[0001] The invention belongs to the technical field of identification of flower disease resistance, in particular to a method for identifying the resistance of different chrysanthemum varieties to chrysanthemum white rust by artificial inoculation in vitro. Background technique

[0002] Chrysanthemum (Dendranthema morifolium) is one of the top ten famous flowers in my country and one of the four fresh cut flowers in the world. It has important humanistic, economic and social values. Chrysanthemum white rust (CWR, chrysanthemum white rust) is a worldwide quarantine disease and one of the most important diseases that harm chrysanthemum.

[0003] Chrysanthemum white rust is caused by Puccinia horiana Henn., a pathogen that mainly damages the leaves of chrysanthemums, with rapid onset and strong destructiveness. The disease was first discovered in Japan in 1895. It was first discovered in Shanghai in 1963 in my country, and then serious occurrences of the disea...