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Method for rapidly and quantitatively detecting Tilletia controversa Kuhn in wheat based on electronic nose

A quantitative detection method, the technology of dwarf smut, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of quantitative detection of unseen wheat, and meet the needs and sensitivity of plant quarantine and disease monitoring High and cost-saving effect

Inactive Publication Date: 2013-08-21
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are research reports on the use of electronic nose to identify TCK in wheat, but there is no research report on the quantitative detection of TCK content in wheat using this technology

Method used

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  • Method for rapidly and quantitatively detecting Tilletia controversa Kuhn in wheat based on electronic nose
  • Method for rapidly and quantitatively detecting Tilletia controversa Kuhn in wheat based on electronic nose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Establishment of a relationship model between TCK teliospore content in unfumigated wheat and the response value of the electronic nose sensor

[0029] 1) Preparation of samples with different TCK teliospore contents in unfumigated wheat

[0030] Weigh 50g of unfumigated wheat seeds and put them into a 100ml Erlenmeyer flask, and use a hemocytometer to make the teliospore concentration of TCK under a microscope into 10 8 ml -1 And after gradient dilution, add in the triangular flask that wheat seed is housed, make the teliospore content in every 50g seed respectively 10 5 、10 4 、10 3 、10 2 、10 1 、10 0 and contrast. Seal with a double layer of plastic wrap.

[0031] 2) Electronic nose measurement method

[0032] Use direct headspace aspiration. That is, the injection needle is directly inserted into a Erlenmeyer flask sealed with double-layer plastic wrap filled with wheat, and the electronic nose is used for determination. The electronic nose uses ...

Embodiment 2

[0039] Example 2: Establishment of relationship model between TCK teliospore content in wheat after fumigation and electronic nose sensor response value

[0040] 1) Preparation of samples with different TCK teliospore contents in wheat after fumigation

[0041] Weigh 50g of fumigated wheat seeds and put them into a 100ml Erlenmeyer flask, and use a hemocytometer to make the teliospore concentration of TCK under a microscope into 10 8 ml -1 And after gradient dilution, add in the triangular flask that wheat seed is housed, make the teliospore content in every 50g seed respectively 10 5 、10 4 、10 3 、10 2 、10 1 and contrast. Seal with a double layer of plastic wrap.

[0042] 2) 2) Electronic nose measurement method

[0043] Use direct headspace aspiration. That is, the injection needle is directly inserted into a Erlenmeyer flask sealed with double-layer plastic wrap filled with wheat, and the electronic nose is used for determination. The electronic nose uses the PEN3 ...

Embodiment 3

[0050] Example 3: Quantitative detection of TCK teliospore content in unfumigated wheat

[0051] 1) Sample preparation for quantitative detection of TCK content in wheat

[0052] Weigh 50g of the wheat sample to be tested and put it into the Erlenmeyer flask, and seal it with double-layer plastic wrap.

[0053] 2) Electronic nose measurement method

[0054] Use direct headspace aspiration. That is, the injection needle is directly inserted into a Erlenmeyer flask sealed with double-layer plastic wrap filled with wheat, and the electronic nose is used for determination. The electronic nose uses the PEN3 electronic nose system produced by the German AIRSENSE company, which contains 10 different metal oxide sensors to form a sensor array.

[0055] 3) Measurement conditions

[0056] The sampling time is 1 second / group, the sensor self-cleaning time is 100 seconds, the sensor zeroing time is 10 seconds, the sample preparation time is 3 seconds, the sample flow rate is 300ml / min...

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Abstract

The invention relates to a method for rapidly and quantitatively detecting Tilletia controversa Kuhn (TCK) in wheat based on an electronic nose, and belongs to the technical field of crop disease control and plant quarantine. In the invention, the quantitative detection of the content of TCK teliospores in an unknown wheat sample is realized through respectively establishing models of relations between the response values of ten sensors in the electronic nose and different TCK teliospore contents in fumigated or unfumigated wheat. The method shortens the time required by the quantitative detection, does not damage a sample for detecting, and can save the quantitative detection cost because no other reagents are basically needed in the detection process. The method can be massively popularized in the TCK quarantine in the ports in China.

Description

(1) Technical field [0001] The "electronic nose-based rapid quantitative detection method for TCK in wheat" of the invention can be used for the rapid quantitative detection of TCK in wheat, and belongs to the technical field of crop disease control and plant quarantine. (2) Background technology [0002] Tilletia controversa Kühn (TCK) is an important worldwide quarantine pathogen, and Tilletia controversa Kühn caused by it is the most harmful and difficult to control quarantine disease one of the diseases. At present, the disease has occurred in more than 30 countries in Europe, America, Asia, and Africa, especially in wheat in the northwestern United States. Wheat dwarf smut is still listed as an A1 quarantine disease that is strictly prohibited from entering my country. [0003] T. tritici can infect more than 60 species of gramineous plants belonging to 15 genera such as wheat, barley, and rye, among which the damage to wheat is the easiest and most serious. The yield...

Claims

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Application Information

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IPC IPC(8): G01N27/00
Inventor 曹学仁周益林段霞瑜陈万权
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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