The use of farnesol in the prevention and treatment of smut
A technology for farnesol and smut, which is applied to the application field of farnesol in the prevention and control of smut, can solve problems such as the inability to effectively prevent and control diseases, and achieve the effect of realizing green and sustainable development.
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Embodiment 1
[0033] On the basis of the preliminary experiment, YEPS liquid medium was used to configure farnesoid into a mother solution with a certain concentration gradient (0 μM, 2.5 μM, 5 μM, 10 μM) for later use. Scrape teliospores from collecting fresh sugarcane smut standard sample again, make teliospore suspension (5×10) with YEPS liquid medium 4 pieces / ml). Pipette 200 μl of different concentrations of mother liquor into 2ml sterilized centrifuge tubes, add an equal volume of teliospore suspension and mix well, and use YEPS without drug as a control. Three repetitions were set for each drug concentration, and each treatment was placed in a shaker at 28°C and 200 rpm / min for 6 hours. After the germination of each teliospore was examined under a microscope, the number of teliospores germinated in 100 teliospores was recorded, and the inhibition rate was calculated.
[0034] Experimental results (see figure 1 ), after culturing in a shaker for 6 hours, the germination rate of the ...
Embodiment 2
[0036]On the basis of the preliminary experiment, the YEPS liquid medium for farnesol was prepared into a mother solution with a certain concentration gradient (0 μM, 0.5 μM, 0.8 μM, 1 μM, 2 μM, 4 μM, 6 μM, 8 μM, 9 μM, 10 μM, 20 μM) for later use. . Pick a little sugarcane smut basidiospores from the flat plate and cultivate overnight in 6mlYEPS nutrient solution as seed liquid, centrifuge the next day to collect the thalline, and resuspend the thalline with YEPS liquid medium (OD 600 0.2) for use. Pipette 200 μl of different concentrations of mother liquor into 2ml sterilized centrifuge tubes, add the same amount of basidiospore suspension and mix well, and use the YEPS liquid medium without adding drugs as the control, and set three replicates for each concentration treatment. Then measure the initial OD of each treatment with a spectrophotometer 600 Afterwards, cultured in a shaker at 28°C and 200rpm / min for 20h, and measured the final OD of each treatment 600 , by calcu...
Embodiment 3
[0041] On the basis of the preliminary experiment, YEPS liquid medium was used to configure farnesoid into a mother solution with a certain concentration gradient (0 μM, 8 μM, 12 μM, 16 μM, 20 μM) for later use. Pick a little sugarcane smut and maize smut basidiospores from the plate respectively and culture them overnight in 6ml YEPS culture solution as the seed solution, and transfer 500 μl to 50ml YEPS culture solution to expand the culture the next day (to OD 600 0.2), pipette 500 μl of different concentrations of mother solution into 5ml sterilized centrifuge tubes, add an equal volume of bacterial solution and mix well, place in 28°C, 200rpm / min shaker for 12h. Then use fresh YEPS liquid medium to dilute the bacterium solution of each concentration treatment by 10 4 times, draw 200 μl and spread it on the YEPSA solid medium, and place it in a 28°C incubator for static culture for 48h. Finally, observe the antibacterial effect and count the number of colonies.
[0042] ...
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