Method for controlling ambrosia trifida by using rust fungus
A technology of ragweed trifida and rust fungus, applied in botany equipment and methods, chemicals for biological control, applications, etc., can solve the problems of high cost, heavy workload, and laboriousness, and achieve low cost, Environmentally safe and easy to operate
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[0015] In July, the leaves of ragweed trifida with teliospores of the rust fungus were collected in the wild, and dried in the shade for later use.
[0016] The seeds of Ambrosia trifidae are soaked in concentrated sulfuric acid with a concentration of 98% for 50s-60s, which can destroy the seed coat of Ambrosia trifidae seeds, and soaked with 0.5% gibberellin for 72 hours; sow in a solar greenhouse , the soil humidity is above 85%, the temperature is 20°C-25°C, and the soil is 3-5cm; when the three-lobed porpoise grows to 4-6 pairs of leaves, it is ready for use.
[0017] Preparation of teliospore suspension: pulverize the dry ragweed trifida leaf with teliospores with a small pulverizer, pass through a 60-mesh sieve, and obtain teliospore coarse powder; add teliospore coarse powder into aqueous solution, and add the final concentration of mass ratio 0.5%-1% xylose or lactose, check with a microscope to make teliospore concentration 10 5 -10 6 pc / ml, which is the standby te...
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