Method for detecting brome Tilletia foetida on broome by using PCR primer

A technology of black powdery mildew and brome, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of inability to perform rapid detection and identification, time-consuming and labor-intensive teliospore germination and cultivation, etc. , to achieve the effect of fast detection, high sensitivity and reliable method
CN101328495AInactive Publication Date: 2008-12-24ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2008-12-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for detecting Tilletia bromi of (Bromus spp.L) by adopting PCR primers, belonging to the Gramineae grass seed medical mycology detection field. The method designs and synthesizes four sets of primers which comprise: a common outer primer, a common inner primer, an outer primer of the T.bromi and a special primer of the T.bromi, wherein, the common outer primer is used for the shell type amplification of the Tilletia hypha genome DNA or teliospore; the common inner primer is used for the shell type amplification of the Tilletia hypha; the two sets of primers are used for the quality monitoring in the amplification process to avoid false negative in the detection process. The T.bromi special primer different from T.controversa, T.caries and T.fusca is designed and is used for the shell type PCR special amplification of the hypha genome DNA conventional PCR and the teliospore, and the outer primer and the special primer perform the shell type amplification on the teliospore DNA. The molecule detection method, which is applied to the T.bromi, is rapid and convenient, has strong specificity and high sensitivity and is accurate and reliable, is established, and the whole detection process can be finished within one working day.
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Description

technical field

[0001] The invention relates to a method for detecting Tilletiabromi parasitizing in brome (Bromus) by using PCR technology. It belongs to the detection field of Tilletia graminearum species. Background technique

[0002] Bromus spp. is an annual or perennial herb, mostly good forage plants, widely distributed in the northern temperate zone of the earth. Among them, brome (B.inermis L) is widely distributed in Northeast China, North China, Northwest China and other places. It has large leaves, good palatability, and rich nutrition. All kinds of livestock like to eat it; Strong resistance to weeds and trampling. It is a high-quality and high-yield forage grass suitable for grassland supplementation and establishment of long-term grasslands. It is also the main grass species for establishing artificial and semi-artificial grasslands in temperate and cold temperate regions in my country; it has strong adaptability to climate. Good drought resistance, heat resist...

Claims

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